Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution

• Published in: Data in Brief
• Main Authors: Boggs, Irina, Hine, Brad, Smolenksi, Grant, Hettinga, Kasper, Zhang, Lina, Wheeler, Thomas T.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2016
• Subjects: Dairy; Drying off; Host defence; Proteomics; Boggs; DML
• Online Access: https://research.wur.nl/en/publications/proteomics-data-in-support-of-the-quantification-of-the-changes-o; https://doi.org/10.1016/j.dib.2016.05.013

Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication "Changes in the repertoire of bovine milk proteins during mammary involution" (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.