Next-generation salmonid alphavirus vaccine development
ABSTRACT Aquaculture is essential to meet the current and future demands for seafood to feed the world population. Atlantic salmon and rainbow trout are two of the most cultured aquaculture species. A pathogen that threatens these species is salmonid alphavirus (SAV). A current inactivated virus vac...
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Wageningen University
2016
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ftunivwagenin:oai:library.wur.nl:wurpubs/503725 2024-02-11T10:02:07+01:00 Next-generation salmonid alphavirus vaccine development Hikke, M.C. Vlak, Just Pijlman, Gorben 2016 application/pdf https://research.wur.nl/en/publications/next-generation-salmonid-alphavirus-vaccine-development https://doi.org/10.18174/371265 en eng Wageningen University https://edepot.wur.nl/371265 https://research.wur.nl/en/publications/next-generation-salmonid-alphavirus-vaccine-development doi:10.18174/371265 Wageningen University & Research alphavirus aquaculture atlantic salmon biotechnology fish culture immunity rainbow trout vaccine development virology alfavirus aquacultuur biotechnologie europese zalm immuniteit regenboogforel vaccinontwikkeling virologie visteelt Doctoral thesis 2016 ftunivwagenin https://doi.org/10.18174/371265 2024-01-24T23:17:53Z ABSTRACT Aquaculture is essential to meet the current and future demands for seafood to feed the world population. Atlantic salmon and rainbow trout are two of the most cultured aquaculture species. A pathogen that threatens these species is salmonid alphavirus (SAV). A current inactivated virus vaccine against SAV provides cross-protection against all SAV subtypes in salmonids and reduces mortality amongst infected fish. However, protection is not 100% and due to virus growth at low temperature, the vaccine production process is time consuming. In addition, the vaccine needs to be injected into the fish, which is a cumbersome process. The work described in this thesis aimed to increase the general knowledge of SAV and to assess current vaccine technologies, and to use this knowledge in designing next-generation vaccines for salmonid aquaculture. An alternative cell line to support SAV proliferation was identified, however, the virus production time could not yet outcompete the current SAV production system. Making use of the baculovirus insect cell expression system, multiple enveloped virus-like particle (eVLP), and core-like particle (CLP) prototype vaccines were produced in insect cells at high temperature. An in vivo vaccination study showed, however, that these vaccines could not readily protect Atlantic salmon against SAV. The low temperature-dependent replication of SAV was attributed to the glycoprotein E2, and it was found that E2 only correctly travelled to the cell surface at low temperature, and in the presence of glycoprotein E1. The biological impact of this finding was confirmed in the development and in vivo testing of a DNA-launched replicon vaccine. The effective DNA-launched replicon vaccine was extended by delivery of the capsid protein in trans. It was hypothesized that viral replicon particles (VRP) were formed in vivo, which would cause an additional single round of infection and might further elevate the immune response in comparison to the replicon vaccine. A second animal trial ... Doctoral or Postdoctoral Thesis Atlantic salmon Wageningen UR (University & Research Centre): Digital Library Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
alphavirus aquaculture atlantic salmon biotechnology fish culture immunity rainbow trout vaccine development virology alfavirus aquacultuur biotechnologie europese zalm immuniteit regenboogforel vaccinontwikkeling virologie visteelt |
spellingShingle |
alphavirus aquaculture atlantic salmon biotechnology fish culture immunity rainbow trout vaccine development virology alfavirus aquacultuur biotechnologie europese zalm immuniteit regenboogforel vaccinontwikkeling virologie visteelt Hikke, M.C. Next-generation salmonid alphavirus vaccine development |
topic_facet |
alphavirus aquaculture atlantic salmon biotechnology fish culture immunity rainbow trout vaccine development virology alfavirus aquacultuur biotechnologie europese zalm immuniteit regenboogforel vaccinontwikkeling virologie visteelt |
description |
ABSTRACT Aquaculture is essential to meet the current and future demands for seafood to feed the world population. Atlantic salmon and rainbow trout are two of the most cultured aquaculture species. A pathogen that threatens these species is salmonid alphavirus (SAV). A current inactivated virus vaccine against SAV provides cross-protection against all SAV subtypes in salmonids and reduces mortality amongst infected fish. However, protection is not 100% and due to virus growth at low temperature, the vaccine production process is time consuming. In addition, the vaccine needs to be injected into the fish, which is a cumbersome process. The work described in this thesis aimed to increase the general knowledge of SAV and to assess current vaccine technologies, and to use this knowledge in designing next-generation vaccines for salmonid aquaculture. An alternative cell line to support SAV proliferation was identified, however, the virus production time could not yet outcompete the current SAV production system. Making use of the baculovirus insect cell expression system, multiple enveloped virus-like particle (eVLP), and core-like particle (CLP) prototype vaccines were produced in insect cells at high temperature. An in vivo vaccination study showed, however, that these vaccines could not readily protect Atlantic salmon against SAV. The low temperature-dependent replication of SAV was attributed to the glycoprotein E2, and it was found that E2 only correctly travelled to the cell surface at low temperature, and in the presence of glycoprotein E1. The biological impact of this finding was confirmed in the development and in vivo testing of a DNA-launched replicon vaccine. The effective DNA-launched replicon vaccine was extended by delivery of the capsid protein in trans. It was hypothesized that viral replicon particles (VRP) were formed in vivo, which would cause an additional single round of infection and might further elevate the immune response in comparison to the replicon vaccine. A second animal trial ... |
author2 |
Vlak, Just Pijlman, Gorben |
format |
Doctoral or Postdoctoral Thesis |
author |
Hikke, M.C. |
author_facet |
Hikke, M.C. |
author_sort |
Hikke, M.C. |
title |
Next-generation salmonid alphavirus vaccine development |
title_short |
Next-generation salmonid alphavirus vaccine development |
title_full |
Next-generation salmonid alphavirus vaccine development |
title_fullStr |
Next-generation salmonid alphavirus vaccine development |
title_full_unstemmed |
Next-generation salmonid alphavirus vaccine development |
title_sort |
next-generation salmonid alphavirus vaccine development |
publisher |
Wageningen University |
publishDate |
2016 |
url |
https://research.wur.nl/en/publications/next-generation-salmonid-alphavirus-vaccine-development https://doi.org/10.18174/371265 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://edepot.wur.nl/371265 https://research.wur.nl/en/publications/next-generation-salmonid-alphavirus-vaccine-development doi:10.18174/371265 |
op_rights |
Wageningen University & Research |
op_doi |
https://doi.org/10.18174/371265 |
_version_ |
1790598024277262336 |