Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study
Following protection measures implemented since the 1970s, large carnivores are currently increasing in number and returning to areas from which they were absent for decades or even centuries. Monitoring programmes for these species rely extensively on non-invasive sampling and genotyping. However,...
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ftunivwagenin:oai:library.wur.nl:wurpubs/495174 2024-04-28T08:15:19+00:00 Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study de Groot, G.A. Nowak, Carsten Skrbinšek, Tomaž Andersen, Liselotte W. Aspi, Jouni Fumagalli, Luca Godinho, Raquel Harms, Verena Jansman, H.A.H. Liberg, Olof Marucco, Francesca Mysłajek, Robert W. Nowak, Sabina Pilot, Małgorzata Randi, Ettore Reinhardt, Ilka Śmietana, Wojciech Szewczyk, Maciej Taberlet, Pierre Vilà, Carles Muñoz-Fuentes, Violeta 2016 application/pdf https://research.wur.nl/en/publications/decades-of-population-genetic-research-reveal-the-need-for-harmon https://doi.org/10.1111/mam.12052 en eng https://edepot.wur.nl/371865 https://research.wur.nl/en/publications/decades-of-population-genetic-research-reveal-the-need-for-harmon doi:10.1111/mam.12052 Wageningen University & Research Mammal Review 46 (2016) 1 ISSN: 0305-1838 Collaboration Genetic monitoring Recommendations Reference collection Transnational research Article/Letter to editor 2016 ftunivwagenin https://doi.org/10.1111/mam.12052 2024-04-03T15:23:10Z Following protection measures implemented since the 1970s, large carnivores are currently increasing in number and returning to areas from which they were absent for decades or even centuries. Monitoring programmes for these species rely extensively on non-invasive sampling and genotyping. However, attempts to connect results of such studies at larger spatial or temporal scales often suffer from the incompatibility of genetic markers implemented by researchers in different laboratories. This is particularly critical for long-distance dispersers, revealing the need for harmonized monitoring schemes that would enable the understanding of gene flow and dispersal dynamics. Based on a review of genetic studies on grey wolves Canis lupus from Europe, we provide an overview of the genetic markers currently in use, and identify opportunities and hurdles for studies based on continent-scale datasets. Our results highlight an urgent need for harmonization of methods to enable transnational research based on data that have already been collected, and to allow these data to be linked to material collected in the future. We suggest timely standardization of newly developed genotyping approaches, and propose that action is directed towards the establishment of shared single nucleotide polymorphism panels, next-generation sequencing of microsatellites, a common reference sample collection and an online database for data exchange. Enhanced cooperation among genetic researchers dealing with large carnivores in consortia would facilitate streamlining of methods, their faster and wider adoption, and production of results at the large spatial scales that ultimately matter for the conservation of these charismatic species. Article in Journal/Newspaper Canis lupus Wageningen UR (University & Research Centre): Digital Library PLOS ONE 11 10 e0163620 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
Collaboration Genetic monitoring Recommendations Reference collection Transnational research |
spellingShingle |
Collaboration Genetic monitoring Recommendations Reference collection Transnational research de Groot, G.A. Nowak, Carsten Skrbinšek, Tomaž Andersen, Liselotte W. Aspi, Jouni Fumagalli, Luca Godinho, Raquel Harms, Verena Jansman, H.A.H. Liberg, Olof Marucco, Francesca Mysłajek, Robert W. Nowak, Sabina Pilot, Małgorzata Randi, Ettore Reinhardt, Ilka Śmietana, Wojciech Szewczyk, Maciej Taberlet, Pierre Vilà, Carles Muñoz-Fuentes, Violeta Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
topic_facet |
Collaboration Genetic monitoring Recommendations Reference collection Transnational research |
description |
Following protection measures implemented since the 1970s, large carnivores are currently increasing in number and returning to areas from which they were absent for decades or even centuries. Monitoring programmes for these species rely extensively on non-invasive sampling and genotyping. However, attempts to connect results of such studies at larger spatial or temporal scales often suffer from the incompatibility of genetic markers implemented by researchers in different laboratories. This is particularly critical for long-distance dispersers, revealing the need for harmonized monitoring schemes that would enable the understanding of gene flow and dispersal dynamics. Based on a review of genetic studies on grey wolves Canis lupus from Europe, we provide an overview of the genetic markers currently in use, and identify opportunities and hurdles for studies based on continent-scale datasets. Our results highlight an urgent need for harmonization of methods to enable transnational research based on data that have already been collected, and to allow these data to be linked to material collected in the future. We suggest timely standardization of newly developed genotyping approaches, and propose that action is directed towards the establishment of shared single nucleotide polymorphism panels, next-generation sequencing of microsatellites, a common reference sample collection and an online database for data exchange. Enhanced cooperation among genetic researchers dealing with large carnivores in consortia would facilitate streamlining of methods, their faster and wider adoption, and production of results at the large spatial scales that ultimately matter for the conservation of these charismatic species. |
format |
Article in Journal/Newspaper |
author |
de Groot, G.A. Nowak, Carsten Skrbinšek, Tomaž Andersen, Liselotte W. Aspi, Jouni Fumagalli, Luca Godinho, Raquel Harms, Verena Jansman, H.A.H. Liberg, Olof Marucco, Francesca Mysłajek, Robert W. Nowak, Sabina Pilot, Małgorzata Randi, Ettore Reinhardt, Ilka Śmietana, Wojciech Szewczyk, Maciej Taberlet, Pierre Vilà, Carles Muñoz-Fuentes, Violeta |
author_facet |
de Groot, G.A. Nowak, Carsten Skrbinšek, Tomaž Andersen, Liselotte W. Aspi, Jouni Fumagalli, Luca Godinho, Raquel Harms, Verena Jansman, H.A.H. Liberg, Olof Marucco, Francesca Mysłajek, Robert W. Nowak, Sabina Pilot, Małgorzata Randi, Ettore Reinhardt, Ilka Śmietana, Wojciech Szewczyk, Maciej Taberlet, Pierre Vilà, Carles Muñoz-Fuentes, Violeta |
author_sort |
de Groot, G.A. |
title |
Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
title_short |
Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
title_full |
Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
title_fullStr |
Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
title_full_unstemmed |
Decades of population genetic research reveal the need for harmonization of molecular markers : The grey wolf Canis lupus as a case study |
title_sort |
decades of population genetic research reveal the need for harmonization of molecular markers : the grey wolf canis lupus as a case study |
publishDate |
2016 |
url |
https://research.wur.nl/en/publications/decades-of-population-genetic-research-reveal-the-need-for-harmon https://doi.org/10.1111/mam.12052 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Mammal Review 46 (2016) 1 ISSN: 0305-1838 |
op_relation |
https://edepot.wur.nl/371865 https://research.wur.nl/en/publications/decades-of-population-genetic-research-reveal-the-need-for-harmon doi:10.1111/mam.12052 |
op_rights |
Wageningen University & Research |
op_doi |
https://doi.org/10.1111/mam.12052 |
container_title |
PLOS ONE |
container_volume |
11 |
container_issue |
10 |
container_start_page |
e0163620 |
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1797580962759966720 |