Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena
DNA was analysed from external wounds on 3 dead harbour porpoises Phocoena phocoena that were stranded in the Netherlands. Puncture wounds as well as the edges of large open wounds were sampled with sterile cotton swabs. With specific primers that target the mtDNA control region of grey seal Halicho...
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Online Access: | https://research.wur.nl/en/publications/detection-of-grey-seal-halichoerus-grypus-dna-in-attack-wounds-on https://doi.org/10.3354/meps11004 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/480405 2024-02-04T10:03:56+01:00 Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena van Bleijswijk, J. Begeman, L. Witte, H.J. IJsseldijk, L. Brasseur, S.M.J.M. Grone, A. Leopold, M.F. 2014 application/pdf https://research.wur.nl/en/publications/detection-of-grey-seal-halichoerus-grypus-dna-in-attack-wounds-on https://doi.org/10.3354/meps11004 en eng https://edepot.wur.nl/326877 https://research.wur.nl/en/publications/detection-of-grey-seal-halichoerus-grypus-dna-in-attack-wounds-on doi:10.3354/meps11004 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Marine Ecology Progress Series 513 (2014) ISSN: 0171-8630 patterns info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2014 ftunivwagenin https://doi.org/10.3354/meps11004 2024-01-10T23:20:09Z DNA was analysed from external wounds on 3 dead harbour porpoises Phocoena phocoena that were stranded in the Netherlands. Puncture wounds as well as the edges of large open wounds were sampled with sterile cotton swabs. With specific primers that target the mtDNA control region of grey seal Halichoerus grypus, a 196 bp DNA fragment was amplified from 4 puncture wounds. Sequencing of the fragments confirmed the presence of grey seal DNA in the puncture wounds. DNA sequences differed between the cases, implying that 3 individual grey seals were involved. As 8 control swabs from intact skin and the transport bag as well as 6 swabs from open wounds on the same harbour porpoises were all negative, contamination with environmental DNA is considered unlikely. The results provide a link between strandings of mutilated harbour porpoises and recent observations of grey seals attacking harbour porpoises. Ours is the first study to use forensic techniques to identify DNA in bite marks from carcasses recovered from the marine environment. This approach can be extended to identify other marine aggressors, including cases involving persons mutilated at sea. Article in Journal/Newspaper Phocoena phocoena Wageningen UR (University & Research Centre): Digital Library Marine Ecology Progress Series 513 277 281 |
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Wageningen UR (University & Research Centre): Digital Library |
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ftunivwagenin |
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English |
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patterns van Bleijswijk, J. Begeman, L. Witte, H.J. IJsseldijk, L. Brasseur, S.M.J.M. Grone, A. Leopold, M.F. Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
topic_facet |
patterns |
description |
DNA was analysed from external wounds on 3 dead harbour porpoises Phocoena phocoena that were stranded in the Netherlands. Puncture wounds as well as the edges of large open wounds were sampled with sterile cotton swabs. With specific primers that target the mtDNA control region of grey seal Halichoerus grypus, a 196 bp DNA fragment was amplified from 4 puncture wounds. Sequencing of the fragments confirmed the presence of grey seal DNA in the puncture wounds. DNA sequences differed between the cases, implying that 3 individual grey seals were involved. As 8 control swabs from intact skin and the transport bag as well as 6 swabs from open wounds on the same harbour porpoises were all negative, contamination with environmental DNA is considered unlikely. The results provide a link between strandings of mutilated harbour porpoises and recent observations of grey seals attacking harbour porpoises. Ours is the first study to use forensic techniques to identify DNA in bite marks from carcasses recovered from the marine environment. This approach can be extended to identify other marine aggressors, including cases involving persons mutilated at sea. |
format |
Article in Journal/Newspaper |
author |
van Bleijswijk, J. Begeman, L. Witte, H.J. IJsseldijk, L. Brasseur, S.M.J.M. Grone, A. Leopold, M.F. |
author_facet |
van Bleijswijk, J. Begeman, L. Witte, H.J. IJsseldijk, L. Brasseur, S.M.J.M. Grone, A. Leopold, M.F. |
author_sort |
van Bleijswijk, J. |
title |
Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
title_short |
Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
title_full |
Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
title_fullStr |
Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
title_full_unstemmed |
Detection of grey seal Halichoerus grypus DNA in attack wounds on stranded harbour porpoises Phocoena phocoena |
title_sort |
detection of grey seal halichoerus grypus dna in attack wounds on stranded harbour porpoises phocoena phocoena |
publishDate |
2014 |
url |
https://research.wur.nl/en/publications/detection-of-grey-seal-halichoerus-grypus-dna-in-attack-wounds-on https://doi.org/10.3354/meps11004 |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Marine Ecology Progress Series 513 (2014) ISSN: 0171-8630 |
op_relation |
https://edepot.wur.nl/326877 https://research.wur.nl/en/publications/detection-of-grey-seal-halichoerus-grypus-dna-in-attack-wounds-on doi:10.3354/meps11004 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.3354/meps11004 |
container_title |
Marine Ecology Progress Series |
container_volume |
513 |
container_start_page |
277 |
op_container_end_page |
281 |
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1789971775402016768 |