Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors
ß-Glucans are glucose polymers that are found in the cell walls of plants, bacteria, certain fungi, mushrooms and the cell wall of baker's yeast. In mammals, myeloid cells express several receptors capable of recognizing ß-glucans, with the C-type lectin receptor dectin-1 in conjunction with To...
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Online Access: | https://research.wur.nl/en/publications/oxidative-and-nitric-oxide-responses-in-carp-macrophages-induced- https://doi.org/10.1016/j.fsi.2013.06.022 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/448767 2024-02-04T09:59:00+01:00 Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors Pietretti, D. Vera-Jimenez, N.I. Hoole, D. Wiegertjes, G.F. 2013 application/pdf https://research.wur.nl/en/publications/oxidative-and-nitric-oxide-responses-in-carp-macrophages-induced- https://doi.org/10.1016/j.fsi.2013.06.022 en eng info:eu-repo/grantAgreement/EC/FP7/214505 https://edepot.wur.nl/290298 https://research.wur.nl/en/publications/oxidative-and-nitric-oxide-responses-in-carp-macrophages-induced- doi:10.1016/j.fsi.2013.06.022 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Fish and Shellfish Immunology 35 (2013) 3 ISSN: 1050-4648 aeromonas-hydrophila atlantic salmon beta-glucan receptor cyprinus-carpio dietary beta-1,3-glucan differential expression polymeric immunoglobulin receptor rainbow-trout scavenger receptors zebrafish danio-rerio info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2013 ftunivwagenin https://doi.org/10.1016/j.fsi.2013.06.022 2024-01-10T23:20:44Z ß-Glucans are glucose polymers that are found in the cell walls of plants, bacteria, certain fungi, mushrooms and the cell wall of baker's yeast. In mammals, myeloid cells express several receptors capable of recognizing ß-glucans, with the C-type lectin receptor dectin-1 in conjunction with Toll-like receptor 2 (TLR2), considered key receptors for recognition of ß-glucan. In our studies to determine the possible involvement of these receptors on carp macrophages a range of sources of ß-glucans were utilized including particulate ß-glucan preparations of baker's yeast such as zymosan, which is composed of insoluble ß-glucan and mannan, and MacroGard(®), a ß-glucan-based feed ingredient for farmed animals including several fish species. Both preparations were confirmed TLR2 ligands by measuring activation of HEK293 cells transfected with human TLR2 and CD14, co-transfected with a secreted embryonic alkaline phosphatase (SEAP) reporter gene. In addition, dectin-1-specific ligands in mammals i.e. zymosan treated to deplete the TLR-stimulating properties and curdlan, were monitored for their effects on carp macrophages by measuring reactive oxygen and nitrogen radicals production, as well as cytokine gene expression by real-time PCR. Results clearly show the ability of carp macrophages to strongly react to particulate ß-glucans with an increase in the production of reactive oxygen and nitrogen radicals and an increase in cytokine gene expression, in particular il-1ß, il-6 and il-11. We identified carp il-6, that was previously unknown. In addition, carp macrophages are less, but not unresponsive to selective dectin-1 agonists, suggesting recognition of ß-glucans by multiple pattern recognition receptors that could include TLR but also non-TLR receptors. Candidate receptors for recognition of ß-glucans are discussed. Article in Journal/Newspaper Atlantic salmon Wageningen UR (University & Research Centre): Digital Library Fish & Shellfish Immunology 35 3 847 857 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
aeromonas-hydrophila atlantic salmon beta-glucan receptor cyprinus-carpio dietary beta-1,3-glucan differential expression polymeric immunoglobulin receptor rainbow-trout scavenger receptors zebrafish danio-rerio |
spellingShingle |
aeromonas-hydrophila atlantic salmon beta-glucan receptor cyprinus-carpio dietary beta-1,3-glucan differential expression polymeric immunoglobulin receptor rainbow-trout scavenger receptors zebrafish danio-rerio Pietretti, D. Vera-Jimenez, N.I. Hoole, D. Wiegertjes, G.F. Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
topic_facet |
aeromonas-hydrophila atlantic salmon beta-glucan receptor cyprinus-carpio dietary beta-1,3-glucan differential expression polymeric immunoglobulin receptor rainbow-trout scavenger receptors zebrafish danio-rerio |
description |
ß-Glucans are glucose polymers that are found in the cell walls of plants, bacteria, certain fungi, mushrooms and the cell wall of baker's yeast. In mammals, myeloid cells express several receptors capable of recognizing ß-glucans, with the C-type lectin receptor dectin-1 in conjunction with Toll-like receptor 2 (TLR2), considered key receptors for recognition of ß-glucan. In our studies to determine the possible involvement of these receptors on carp macrophages a range of sources of ß-glucans were utilized including particulate ß-glucan preparations of baker's yeast such as zymosan, which is composed of insoluble ß-glucan and mannan, and MacroGard(®), a ß-glucan-based feed ingredient for farmed animals including several fish species. Both preparations were confirmed TLR2 ligands by measuring activation of HEK293 cells transfected with human TLR2 and CD14, co-transfected with a secreted embryonic alkaline phosphatase (SEAP) reporter gene. In addition, dectin-1-specific ligands in mammals i.e. zymosan treated to deplete the TLR-stimulating properties and curdlan, were monitored for their effects on carp macrophages by measuring reactive oxygen and nitrogen radicals production, as well as cytokine gene expression by real-time PCR. Results clearly show the ability of carp macrophages to strongly react to particulate ß-glucans with an increase in the production of reactive oxygen and nitrogen radicals and an increase in cytokine gene expression, in particular il-1ß, il-6 and il-11. We identified carp il-6, that was previously unknown. In addition, carp macrophages are less, but not unresponsive to selective dectin-1 agonists, suggesting recognition of ß-glucans by multiple pattern recognition receptors that could include TLR but also non-TLR receptors. Candidate receptors for recognition of ß-glucans are discussed. |
format |
Article in Journal/Newspaper |
author |
Pietretti, D. Vera-Jimenez, N.I. Hoole, D. Wiegertjes, G.F. |
author_facet |
Pietretti, D. Vera-Jimenez, N.I. Hoole, D. Wiegertjes, G.F. |
author_sort |
Pietretti, D. |
title |
Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
title_short |
Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
title_full |
Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
title_fullStr |
Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
title_full_unstemmed |
Oxidative and nitric oxide responses in carp macrophages induced by zymosan, MacroGard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
title_sort |
oxidative and nitric oxide responses in carp macrophages induced by zymosan, macrogard and selective dectin-1 agonists suggest recognition by multiple pattern recognition receptors |
publishDate |
2013 |
url |
https://research.wur.nl/en/publications/oxidative-and-nitric-oxide-responses-in-carp-macrophages-induced- https://doi.org/10.1016/j.fsi.2013.06.022 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Fish and Shellfish Immunology 35 (2013) 3 ISSN: 1050-4648 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/214505 https://edepot.wur.nl/290298 https://research.wur.nl/en/publications/oxidative-and-nitric-oxide-responses-in-carp-macrophages-induced- doi:10.1016/j.fsi.2013.06.022 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/j.fsi.2013.06.022 |
container_title |
Fish & Shellfish Immunology |
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35 |
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3 |
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847 |
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857 |
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