Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii)
We investigated the effects of administration of putative endogenous probiotics Lactococcus lactis spp. lactis or Bacillus circulans, alone and in combination with arabinoxylan-oligosaccharides (AXOS), a new class of candidate prebiotics, in juvenile Siberian sturgeon (Acipenser baerii). Eight exper...
Published in: | Fish & Shellfish Immunology |
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2013
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Online Access: | https://research.wur.nl/en/publications/effects-of-dietary-arabinoxylan-oligosaccharides-axos-and-endogen https://doi.org/10.1016/j.fsi.2013.06.014 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/441661 2024-01-14T09:58:34+01:00 Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) Geraylou, Z. Souffreau, C. Rurangwa, E. de Meester, L. Courtin, C.M. Delcour, J.A. Buyse, J. Ollevier, F. 2013 application/pdf https://research.wur.nl/en/publications/effects-of-dietary-arabinoxylan-oligosaccharides-axos-and-endogen https://doi.org/10.1016/j.fsi.2013.06.014 en eng https://edepot.wur.nl/265888 https://research.wur.nl/en/publications/effects-of-dietary-arabinoxylan-oligosaccharides-axos-and-endogen doi:10.1016/j.fsi.2013.06.014 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Fish and Shellfish Immunology 35 (2013) 3 ISSN: 1050-4648 bacillus-subtilis disease resistance fish in-vitro intestinal microbiota labeo-rohita lactic-acid bacteria lactococcus-lactis scophthalmus-maximus supplementation info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2013 ftunivwagenin https://doi.org/10.1016/j.fsi.2013.06.014 2023-12-20T23:18:33Z We investigated the effects of administration of putative endogenous probiotics Lactococcus lactis spp. lactis or Bacillus circulans, alone and in combination with arabinoxylan-oligosaccharides (AXOS), a new class of candidate prebiotics, in juvenile Siberian sturgeon (Acipenser baerii). Eight experimental diets were tested: basal diet (Diet 1), basal diet supplemented with 2% AXOS (Diet 2), or L. lactis ST G81 (Diet 3), L. lactis ST G45 (Diet 4), B. circulans ST M53 (Diet 5), L. lactis ST G81 + 2% AXOS (Diet 6), L. lactis ST G45 + 2% AXOS (Diet 7), B. circulans ST M53 + 2% AXOS (Diet 8). After four weeks, growth performance and feed conversion ratio significantly improved in fish fed diet 7. Innate immune responses of fish were boosted with both AXOS and probiotic diets, however synergistic effects of AXOS and probiotic diets were only observed for phagocytic and alternative complement activity. Phagocytic and respiratory burst activity of fish macrophage increased in fish fed diet 2 and 7, while humoral immune responses only increased in fish fed diet 7. Pyrosequencing analysis (16S rDNA) of the hindgut microbiota demonstrated that AXOS improved the colonization or/and growth capacity of L. lactis, as a higher relative abundance of L. lactis was observed in fish receiving diet 7. However, no observable colonization of B. circulans was found in the hindgut of fish fed diet 5 or 8, containing this bacterium. The dietary L. lactis ST G45 + 2% AXOS caused significant alterations in the intestinal microbiota by significantly decreasing in bacterial diversity, demonstrated by the fall in richness and Shannon diversity, and improved growth performance and boosted immune responses of Siberian sturgeon. Article in Journal/Newspaper Acipenser baerii Scophthalmus maximus Siberian sturgeon Wageningen UR (University & Research Centre): Digital Library Fish & Shellfish Immunology 35 3 766 775 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
bacillus-subtilis disease resistance fish in-vitro intestinal microbiota labeo-rohita lactic-acid bacteria lactococcus-lactis scophthalmus-maximus supplementation |
spellingShingle |
bacillus-subtilis disease resistance fish in-vitro intestinal microbiota labeo-rohita lactic-acid bacteria lactococcus-lactis scophthalmus-maximus supplementation Geraylou, Z. Souffreau, C. Rurangwa, E. de Meester, L. Courtin, C.M. Delcour, J.A. Buyse, J. Ollevier, F. Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
topic_facet |
bacillus-subtilis disease resistance fish in-vitro intestinal microbiota labeo-rohita lactic-acid bacteria lactococcus-lactis scophthalmus-maximus supplementation |
description |
We investigated the effects of administration of putative endogenous probiotics Lactococcus lactis spp. lactis or Bacillus circulans, alone and in combination with arabinoxylan-oligosaccharides (AXOS), a new class of candidate prebiotics, in juvenile Siberian sturgeon (Acipenser baerii). Eight experimental diets were tested: basal diet (Diet 1), basal diet supplemented with 2% AXOS (Diet 2), or L. lactis ST G81 (Diet 3), L. lactis ST G45 (Diet 4), B. circulans ST M53 (Diet 5), L. lactis ST G81 + 2% AXOS (Diet 6), L. lactis ST G45 + 2% AXOS (Diet 7), B. circulans ST M53 + 2% AXOS (Diet 8). After four weeks, growth performance and feed conversion ratio significantly improved in fish fed diet 7. Innate immune responses of fish were boosted with both AXOS and probiotic diets, however synergistic effects of AXOS and probiotic diets were only observed for phagocytic and alternative complement activity. Phagocytic and respiratory burst activity of fish macrophage increased in fish fed diet 2 and 7, while humoral immune responses only increased in fish fed diet 7. Pyrosequencing analysis (16S rDNA) of the hindgut microbiota demonstrated that AXOS improved the colonization or/and growth capacity of L. lactis, as a higher relative abundance of L. lactis was observed in fish receiving diet 7. However, no observable colonization of B. circulans was found in the hindgut of fish fed diet 5 or 8, containing this bacterium. The dietary L. lactis ST G45 + 2% AXOS caused significant alterations in the intestinal microbiota by significantly decreasing in bacterial diversity, demonstrated by the fall in richness and Shannon diversity, and improved growth performance and boosted immune responses of Siberian sturgeon. |
format |
Article in Journal/Newspaper |
author |
Geraylou, Z. Souffreau, C. Rurangwa, E. de Meester, L. Courtin, C.M. Delcour, J.A. Buyse, J. Ollevier, F. |
author_facet |
Geraylou, Z. Souffreau, C. Rurangwa, E. de Meester, L. Courtin, C.M. Delcour, J.A. Buyse, J. Ollevier, F. |
author_sort |
Geraylou, Z. |
title |
Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
title_short |
Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
title_full |
Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
title_fullStr |
Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
title_full_unstemmed |
Effects of dietary arabinoxylan-oligosaccharides (AXOS) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile Siberian sturgeon (Acipenser baerii) |
title_sort |
effects of dietary arabinoxylan-oligosaccharides (axos) and endogenous probiotics on the growth performance, non-specific immunity and gut micrbiota of juvenile siberian sturgeon (acipenser baerii) |
publishDate |
2013 |
url |
https://research.wur.nl/en/publications/effects-of-dietary-arabinoxylan-oligosaccharides-axos-and-endogen https://doi.org/10.1016/j.fsi.2013.06.014 |
genre |
Acipenser baerii Scophthalmus maximus Siberian sturgeon |
genre_facet |
Acipenser baerii Scophthalmus maximus Siberian sturgeon |
op_source |
Fish and Shellfish Immunology 35 (2013) 3 ISSN: 1050-4648 |
op_relation |
https://edepot.wur.nl/265888 https://research.wur.nl/en/publications/effects-of-dietary-arabinoxylan-oligosaccharides-axos-and-endogen doi:10.1016/j.fsi.2013.06.014 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/j.fsi.2013.06.014 |
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Fish & Shellfish Immunology |
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35 |
container_issue |
3 |
container_start_page |
766 |
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775 |
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1788067235839868928 |