Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding
In this study, we described the partial structure, mRNA tissue distribution and regulation of two carp mucin and two ß-defensin genes. This is the first description of these genes in fish. The genes might provide relevant tools to monitor feed-related improvements of fish health under aquaculture co...
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Online Access: | https://research.wur.nl/en/publications/molecular-cloning-and-expression-of-two-b-defensin-and-two-mucin- https://doi.org/10.1016/j.fsi.2011.12.008 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/427533 2024-02-04T09:58:58+01:00 Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding van der Marel, M.C. Adamek, M. Gonzalez, S.F. Frost, P. Rombout, J.H.W.M. Wiegertjes, G.F. Savelkoul, H.F.J. Steinhagen, D. 2012 application/pdf https://research.wur.nl/en/publications/molecular-cloning-and-expression-of-two-b-defensin-and-two-mucin- https://doi.org/10.1016/j.fsi.2011.12.008 en eng info:eu-repo/grantAgreement/EC/FP7/214505 https://edepot.wur.nl/222914 https://research.wur.nl/en/publications/molecular-cloning-and-expression-of-two-b-defensin-and-two-mucin- doi:10.1016/j.fsi.2011.12.008 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Fish and Shellfish Immunology 32 (2012) 3 ISSN: 1050-4648 atlantic salmon disease resistance fish gram-negative bacteria immune-responses infection macrophages microbiota salmo-salar l secreted mucins info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2012 ftunivwagenin https://doi.org/10.1016/j.fsi.2011.12.008 2024-01-10T23:21:15Z In this study, we described the partial structure, mRNA tissue distribution and regulation of two carp mucin and two ß-defensin genes. This is the first description of these genes in fish. The genes might provide relevant tools to monitor feed-related improvements of fish health under aquaculture conditions. Carp mucin 2 and mucin 5B genes show a high similarity to their mammalian and avian counterparts. The carp ß-defensin 1 and ß-defensin 2 genes cluster together well with their piscine family members. The influence of a ß-glucan immunomodulant on the expression of these genes in mucosal tissues could be confirmed for the first time. Muc5B expression was significantly increased in the skin. For Muc2 no significant up- or down-regulation could be observed. Significantly higher expression levels of ß-defensin 2 in gills and both ß-defensin genes in skin were found. Thus, the mucosal system can be influenced by the addition of ß-glucans to the food Article in Journal/Newspaper Atlantic salmon Salmo salar Wageningen UR (University & Research Centre): Digital Library Fish & Shellfish Immunology 32 3 494 501 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
atlantic salmon disease resistance fish gram-negative bacteria immune-responses infection macrophages microbiota salmo-salar l secreted mucins |
spellingShingle |
atlantic salmon disease resistance fish gram-negative bacteria immune-responses infection macrophages microbiota salmo-salar l secreted mucins van der Marel, M.C. Adamek, M. Gonzalez, S.F. Frost, P. Rombout, J.H.W.M. Wiegertjes, G.F. Savelkoul, H.F.J. Steinhagen, D. Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
topic_facet |
atlantic salmon disease resistance fish gram-negative bacteria immune-responses infection macrophages microbiota salmo-salar l secreted mucins |
description |
In this study, we described the partial structure, mRNA tissue distribution and regulation of two carp mucin and two ß-defensin genes. This is the first description of these genes in fish. The genes might provide relevant tools to monitor feed-related improvements of fish health under aquaculture conditions. Carp mucin 2 and mucin 5B genes show a high similarity to their mammalian and avian counterparts. The carp ß-defensin 1 and ß-defensin 2 genes cluster together well with their piscine family members. The influence of a ß-glucan immunomodulant on the expression of these genes in mucosal tissues could be confirmed for the first time. Muc5B expression was significantly increased in the skin. For Muc2 no significant up- or down-regulation could be observed. Significantly higher expression levels of ß-defensin 2 in gills and both ß-defensin genes in skin were found. Thus, the mucosal system can be influenced by the addition of ß-glucans to the food |
format |
Article in Journal/Newspaper |
author |
van der Marel, M.C. Adamek, M. Gonzalez, S.F. Frost, P. Rombout, J.H.W.M. Wiegertjes, G.F. Savelkoul, H.F.J. Steinhagen, D. |
author_facet |
van der Marel, M.C. Adamek, M. Gonzalez, S.F. Frost, P. Rombout, J.H.W.M. Wiegertjes, G.F. Savelkoul, H.F.J. Steinhagen, D. |
author_sort |
van der Marel, M.C. |
title |
Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
title_short |
Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
title_full |
Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
title_fullStr |
Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
title_full_unstemmed |
Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding |
title_sort |
molecular cloning and expression of two b-defensin and two mucin genes in common carp (cyprinus carpio l.) and their up-regulation after b-glucan feeding |
publishDate |
2012 |
url |
https://research.wur.nl/en/publications/molecular-cloning-and-expression-of-two-b-defensin-and-two-mucin- https://doi.org/10.1016/j.fsi.2011.12.008 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Fish and Shellfish Immunology 32 (2012) 3 ISSN: 1050-4648 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/214505 https://edepot.wur.nl/222914 https://research.wur.nl/en/publications/molecular-cloning-and-expression-of-two-b-defensin-and-two-mucin- doi:10.1016/j.fsi.2011.12.008 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/j.fsi.2011.12.008 |
container_title |
Fish & Shellfish Immunology |
container_volume |
32 |
container_issue |
3 |
container_start_page |
494 |
op_container_end_page |
501 |
_version_ |
1789963574236413952 |