Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation
Pancreas disease (PD) and sleeping disease (SD) are important viral scourges in aquaculture of Atlantic salmon and rainbow trout. The etiological agent of PD and SD is salmonid alphavirus (SAV), an unusual member of the Togaviridae (genus Alphavirus). SAV replicates at lower temperatures in fish. Ou...
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ftunivwagenin:oai:library.wur.nl:wurpubs/421065 2024-02-04T09:58:56+01:00 Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation Metz, S.W.H. Feenstra, F. Villoing, S. van Hulten, M.C. van Lent, J.W.M. Koumans, J. Vlak, J.M. Pijlman, G.P. 2011 application/pdf https://research.wur.nl/en/publications/low-temperature-dependent-salmonid-alphavirus-glycoprotein-proces https://doi.org/10.1371/journal.pone.0025816 en eng https://edepot.wur.nl/195213 https://research.wur.nl/en/publications/low-temperature-dependent-salmonid-alphavirus-glycoprotein-proces doi:10.1371/journal.pone.0025816 info:eu-repo/semantics/openAccess Wageningen University & Research PLoS ONE 6 (2011) 10 ISSN: 1932-6203 atlantic salmon baculovirus vectors equine encephalitis-virus nuclear polyhedrosis-virus nucleocapsid protein pancreas disease semliki-forest-virus sleeping-disease spodoptera-frugiperda swine-fever virus info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2011 ftunivwagenin https://doi.org/10.1371/journal.pone.0025816 2024-01-10T23:22:14Z Pancreas disease (PD) and sleeping disease (SD) are important viral scourges in aquaculture of Atlantic salmon and rainbow trout. The etiological agent of PD and SD is salmonid alphavirus (SAV), an unusual member of the Togaviridae (genus Alphavirus). SAV replicates at lower temperatures in fish. Outbreaks of SAV are associated with large economic losses of ~17 to 50 million $/year. Current control strategies rely on vaccination with inactivated virus formulations that are cumbersome to obtain and have intrinsic safety risks. In this research we were able to obtain non-infectious virus-like particles (VLPs) of SAV via expression of recombinant baculoviruses encoding SAV capsid protein and two major immunodominant viral glycoproteins, E1 and E2 in Spodoptera frugiperda Sf9 insect cells. However, this was only achieved when a temperature shift from 27°C to lower temperatures was applied. At 27°C, precursor E2 (PE2) was misfolded and not processed by host furin into mature E2. Hence, E2 was detected neither on the surface of infected cells nor as VLPs in the culture fluid. However, when temperatures during protein expression were lowered, PE2 was processed into mature E2 in a temperature-dependent manner and VLPs were abundantly produced. So, temperature shift-down during synthesis is a prerequisite for correct SAV glycoprotein processing and recombinant VLP production Article in Journal/Newspaper Atlantic salmon Wageningen UR (University & Research Centre): Digital Library Sav’ ENVELOPE(156.400,156.400,68.817,68.817) PLoS ONE 6 10 e25816 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
atlantic salmon baculovirus vectors equine encephalitis-virus nuclear polyhedrosis-virus nucleocapsid protein pancreas disease semliki-forest-virus sleeping-disease spodoptera-frugiperda swine-fever virus |
spellingShingle |
atlantic salmon baculovirus vectors equine encephalitis-virus nuclear polyhedrosis-virus nucleocapsid protein pancreas disease semliki-forest-virus sleeping-disease spodoptera-frugiperda swine-fever virus Metz, S.W.H. Feenstra, F. Villoing, S. van Hulten, M.C. van Lent, J.W.M. Koumans, J. Vlak, J.M. Pijlman, G.P. Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
topic_facet |
atlantic salmon baculovirus vectors equine encephalitis-virus nuclear polyhedrosis-virus nucleocapsid protein pancreas disease semliki-forest-virus sleeping-disease spodoptera-frugiperda swine-fever virus |
description |
Pancreas disease (PD) and sleeping disease (SD) are important viral scourges in aquaculture of Atlantic salmon and rainbow trout. The etiological agent of PD and SD is salmonid alphavirus (SAV), an unusual member of the Togaviridae (genus Alphavirus). SAV replicates at lower temperatures in fish. Outbreaks of SAV are associated with large economic losses of ~17 to 50 million $/year. Current control strategies rely on vaccination with inactivated virus formulations that are cumbersome to obtain and have intrinsic safety risks. In this research we were able to obtain non-infectious virus-like particles (VLPs) of SAV via expression of recombinant baculoviruses encoding SAV capsid protein and two major immunodominant viral glycoproteins, E1 and E2 in Spodoptera frugiperda Sf9 insect cells. However, this was only achieved when a temperature shift from 27°C to lower temperatures was applied. At 27°C, precursor E2 (PE2) was misfolded and not processed by host furin into mature E2. Hence, E2 was detected neither on the surface of infected cells nor as VLPs in the culture fluid. However, when temperatures during protein expression were lowered, PE2 was processed into mature E2 in a temperature-dependent manner and VLPs were abundantly produced. So, temperature shift-down during synthesis is a prerequisite for correct SAV glycoprotein processing and recombinant VLP production |
format |
Article in Journal/Newspaper |
author |
Metz, S.W.H. Feenstra, F. Villoing, S. van Hulten, M.C. van Lent, J.W.M. Koumans, J. Vlak, J.M. Pijlman, G.P. |
author_facet |
Metz, S.W.H. Feenstra, F. Villoing, S. van Hulten, M.C. van Lent, J.W.M. Koumans, J. Vlak, J.M. Pijlman, G.P. |
author_sort |
Metz, S.W.H. |
title |
Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
title_short |
Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
title_full |
Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
title_fullStr |
Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
title_full_unstemmed |
Low Temperature-Dependent Salmonid Alphavirus Glycoprotein Processing and Recombinant Virus-Like Particle Formation |
title_sort |
low temperature-dependent salmonid alphavirus glycoprotein processing and recombinant virus-like particle formation |
publishDate |
2011 |
url |
https://research.wur.nl/en/publications/low-temperature-dependent-salmonid-alphavirus-glycoprotein-proces https://doi.org/10.1371/journal.pone.0025816 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE 6 (2011) 10 ISSN: 1932-6203 |
op_relation |
https://edepot.wur.nl/195213 https://research.wur.nl/en/publications/low-temperature-dependent-salmonid-alphavirus-glycoprotein-proces doi:10.1371/journal.pone.0025816 |
op_rights |
info:eu-repo/semantics/openAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1371/journal.pone.0025816 |
container_title |
PLoS ONE |
container_volume |
6 |
container_issue |
10 |
container_start_page |
e25816 |
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1789963531539447808 |