Immobilization to prevent enzyme incompatibility with proteases

• Published in: Biocatalysis and Biotransformation
• Main Authors: Vossenberg, P., Beeftink, H.H., Cohen Stuart, M.A., Tramper, J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2011
• Subjects: autolysis; candida-antarctica; crystal-structure; deactivation; improvement; lipase-b; organic-synthesis; peptide-synthesis; proteins; water activity; Antarc*; Antarctica
• Online Access: https://research.wur.nl/en/publications/immobilization-to-prevent-enzyme-incompatibility-with-proteases; https://doi.org/10.3109/10242422.2011.631213

Enzyme incompatibility is a problem in multi-enzyme processes that involve a non-specific protease, such as Alcalase. An example is the one-pot enzymatic synthesis of peptides catalyzed by a lipase and a protease. The incompatibility between lipase B from Candida antarctica (CalB) and Alcalase was studied. To what extent immobilization of both or either CalB or Alcalase onto macroporous beads helps to prevent hydrolysis of CalB by Alcalase was evaluated. The rate of activity loss of native and immobilized CalB in the absence and presence of native and immobilized Alcalase was calculated from the rate of triacetin hydrolysis. Immobilization of both or either CalB or Alcalase onto macroporous beads was found to be effective in largely preventing hydrolysis of CalB by Alcalase