Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats
We studied the presence of hydroxylated metabolites of hexabromocyclododecane (HBCD) in three wildlife species (tern egg, seal, and flounder) and in Wistar rats exposed to 30 and 100 mg HBCD/kg bw/day for 28 days. A nondestructive extraction, fractionation, and cleanup method was developed to separa...
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ftunivwagenin:oai:library.wur.nl:wurpubs/395329 2024-02-04T10:00:59+01:00 Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats Brandsma, S.H. van der Ven, L.T.M. de Boer, J. Leonards, P.E.G. 2009 application/pdf https://research.wur.nl/en/publications/identification-of-hydroxylated-metabolites-of-hexabromocyclododec https://doi.org/10.1021/es900879k en eng https://edepot.wur.nl/148166 https://research.wur.nl/en/publications/identification-of-hydroxylated-metabolites-of-hexabromocyclododec doi:10.1021/es900879k info:eu-repo/semantics/restrictedAccess Wageningen University & Research Environmental Science and Technology 43 (2009) 15 ISSN: 0013-936X fish mass-spectrometry products thermal-stability info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2009 ftunivwagenin https://doi.org/10.1021/es900879k 2024-01-10T23:23:29Z We studied the presence of hydroxylated metabolites of hexabromocyclododecane (HBCD) in three wildlife species (tern egg, seal, and flounder) and in Wistar rats exposed to 30 and 100 mg HBCD/kg bw/day for 28 days. A nondestructive extraction, fractionation, and cleanup method was developed to separate the hydroxylated HBCD metabolites from the biotic sample matrix. Four different groups of hydroxylated HBCD metabolites were identified in rat adipose, liver, lung, and muscle tissues by liquid and gas chromatography (LC and GC) combined with mass spectrometry (MS): monohydroxy metabolites of HBCD, pentabromocyclododecene (PBCDe), tetrabromocyclododecene (TBCDe), and dihydroxy-HBCD. Dihydroxy-PBCDe was identified by GC-MS but could not be confirmed by LC-MS. Debromination of HBCD to PBCDe was another metabolic pathway observed. In tern eggs from the Western Scheldt the monohydroxy-HBCD was found and in the blubber of harbor seal (Wadden Sea) the monohydroxy metabolites of HBCD and PBCDe were found. No hydroxylated metabolites were detected in the tissue of flounder (Wadden Sea). To our knowledge, this is the first study to identify different hydroxylated metabolite groups of HBCD in rat and wildlife samples. Article in Journal/Newspaper harbor seal Wageningen UR (University & Research Centre): Digital Library Environmental Science & Technology 43 15 6058 6063 |
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Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
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ftunivwagenin |
language |
English |
topic |
fish mass-spectrometry products thermal-stability |
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fish mass-spectrometry products thermal-stability Brandsma, S.H. van der Ven, L.T.M. de Boer, J. Leonards, P.E.G. Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
topic_facet |
fish mass-spectrometry products thermal-stability |
description |
We studied the presence of hydroxylated metabolites of hexabromocyclododecane (HBCD) in three wildlife species (tern egg, seal, and flounder) and in Wistar rats exposed to 30 and 100 mg HBCD/kg bw/day for 28 days. A nondestructive extraction, fractionation, and cleanup method was developed to separate the hydroxylated HBCD metabolites from the biotic sample matrix. Four different groups of hydroxylated HBCD metabolites were identified in rat adipose, liver, lung, and muscle tissues by liquid and gas chromatography (LC and GC) combined with mass spectrometry (MS): monohydroxy metabolites of HBCD, pentabromocyclododecene (PBCDe), tetrabromocyclododecene (TBCDe), and dihydroxy-HBCD. Dihydroxy-PBCDe was identified by GC-MS but could not be confirmed by LC-MS. Debromination of HBCD to PBCDe was another metabolic pathway observed. In tern eggs from the Western Scheldt the monohydroxy-HBCD was found and in the blubber of harbor seal (Wadden Sea) the monohydroxy metabolites of HBCD and PBCDe were found. No hydroxylated metabolites were detected in the tissue of flounder (Wadden Sea). To our knowledge, this is the first study to identify different hydroxylated metabolite groups of HBCD in rat and wildlife samples. |
format |
Article in Journal/Newspaper |
author |
Brandsma, S.H. van der Ven, L.T.M. de Boer, J. Leonards, P.E.G. |
author_facet |
Brandsma, S.H. van der Ven, L.T.M. de Boer, J. Leonards, P.E.G. |
author_sort |
Brandsma, S.H. |
title |
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
title_short |
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
title_full |
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
title_fullStr |
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
title_full_unstemmed |
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats |
title_sort |
identification of hydroxylated metabolites of hexabromocyclododecane in wildlife and 28-days exposed wistar rats |
publishDate |
2009 |
url |
https://research.wur.nl/en/publications/identification-of-hydroxylated-metabolites-of-hexabromocyclododec https://doi.org/10.1021/es900879k |
genre |
harbor seal |
genre_facet |
harbor seal |
op_source |
Environmental Science and Technology 43 (2009) 15 ISSN: 0013-936X |
op_relation |
https://edepot.wur.nl/148166 https://research.wur.nl/en/publications/identification-of-hydroxylated-metabolites-of-hexabromocyclododec doi:10.1021/es900879k |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1021/es900879k |
container_title |
Environmental Science & Technology |
container_volume |
43 |
container_issue |
15 |
container_start_page |
6058 |
op_container_end_page |
6063 |
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1789966578412945408 |