Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation
Matrix metalloproteinase 9 (MMP-9) belongs to a family of zinc-dependent endopeptidases. As a consequence of its ability to cleave structural extracellular matrix molecules, mammalian MMP-9 is associated with vital inflammatory processes such as leucocyte migration and tissue remodelling and regener...
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Online Access: | https://research.wur.nl/en/publications/expression-profiles-of-matrix-metalloproteinase-9-in-teleost-fish https://doi.org/10.1111/j.1365-2567.2008.02874.x |
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ftunivwagenin:oai:library.wur.nl:wurpubs/372044 2024-02-04T10:00:30+01:00 Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation Chadzinska, M.K. Baginski, P. Kolaczkowska, E. Savelkoul, H.F.J. Verburg-van Kemenade, B.M.L. 2008 application/pdf https://research.wur.nl/en/publications/expression-profiles-of-matrix-metalloproteinase-9-in-teleost-fish https://doi.org/10.1111/j.1365-2567.2008.02874.x en eng https://edepot.wur.nl/23993 https://research.wur.nl/en/publications/expression-profiles-of-matrix-metalloproteinase-9-in-teleost-fish doi:10.1111/j.1365-2567.2008.02874.x info:eu-repo/semantics/restrictedAccess Wageningen University & Research Immunology 125 (2008) 4 ISSN: 0019-2805 cod gadus-morhua cyprinus-carpio l gelatinolytic activities induced peritoneal inflammation lymphoid organs mast-cells matrix-metalloproteinase inhibitors neutrophil gelatinase b spawning stage zymosan peritonitis info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2008 ftunivwagenin https://doi.org/10.1111/j.1365-2567.2008.02874.x 2024-01-10T23:24:25Z Matrix metalloproteinase 9 (MMP-9) belongs to a family of zinc-dependent endopeptidases. As a consequence of its ability to cleave structural extracellular matrix molecules, mammalian MMP-9 is associated with vital inflammatory processes such as leucocyte migration and tissue remodelling and regeneration. Interestingly, MMP-9 genes have been identified in fish, but functional data are still limited and focus on the involvement of MMP-9 in embryonic development, reproduction and post-mortem tenderization. Here, we describe the involvement of MMP-9 in the innate immunity of carp. In carp, MMP-9 was most notably expressed in classical fish immune organs and in peritoneal and peripheral blood leucocytes, indicating a role of MMP-9 in immune responses. In our well-characterized zymosan-induced peritonitis model for carp, we analysed expression of the MMP-9 gene and the gelatinolytic levels of both pro- and activated forms of MMP-9. The biphasic profile of MMP-9 mRNA expression indicated involvement during the initial phase of inflammation and during the later phase of tissue remodelling. Also, in vitro stimulation of carp phagocytes with lipopolysaccharide or concanavalin A increased MMP-9 gene expression, with a peak at 24¿hr. The increase of MMP-9 mRNA correlated with the peak of MMP-9 gelatinolytic level in culture supernatants. These results provide evidence for an evolutionarily conserved and relevant role of MMP-9 in the innate immune response. Article in Journal/Newspaper Gadus morhua Wageningen UR (University & Research Centre): Digital Library Immunology 125 4 601 610 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
cod gadus-morhua cyprinus-carpio l gelatinolytic activities induced peritoneal inflammation lymphoid organs mast-cells matrix-metalloproteinase inhibitors neutrophil gelatinase b spawning stage zymosan peritonitis |
spellingShingle |
cod gadus-morhua cyprinus-carpio l gelatinolytic activities induced peritoneal inflammation lymphoid organs mast-cells matrix-metalloproteinase inhibitors neutrophil gelatinase b spawning stage zymosan peritonitis Chadzinska, M.K. Baginski, P. Kolaczkowska, E. Savelkoul, H.F.J. Verburg-van Kemenade, B.M.L. Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
topic_facet |
cod gadus-morhua cyprinus-carpio l gelatinolytic activities induced peritoneal inflammation lymphoid organs mast-cells matrix-metalloproteinase inhibitors neutrophil gelatinase b spawning stage zymosan peritonitis |
description |
Matrix metalloproteinase 9 (MMP-9) belongs to a family of zinc-dependent endopeptidases. As a consequence of its ability to cleave structural extracellular matrix molecules, mammalian MMP-9 is associated with vital inflammatory processes such as leucocyte migration and tissue remodelling and regeneration. Interestingly, MMP-9 genes have been identified in fish, but functional data are still limited and focus on the involvement of MMP-9 in embryonic development, reproduction and post-mortem tenderization. Here, we describe the involvement of MMP-9 in the innate immunity of carp. In carp, MMP-9 was most notably expressed in classical fish immune organs and in peritoneal and peripheral blood leucocytes, indicating a role of MMP-9 in immune responses. In our well-characterized zymosan-induced peritonitis model for carp, we analysed expression of the MMP-9 gene and the gelatinolytic levels of both pro- and activated forms of MMP-9. The biphasic profile of MMP-9 mRNA expression indicated involvement during the initial phase of inflammation and during the later phase of tissue remodelling. Also, in vitro stimulation of carp phagocytes with lipopolysaccharide or concanavalin A increased MMP-9 gene expression, with a peak at 24¿hr. The increase of MMP-9 mRNA correlated with the peak of MMP-9 gelatinolytic level in culture supernatants. These results provide evidence for an evolutionarily conserved and relevant role of MMP-9 in the innate immune response. |
format |
Article in Journal/Newspaper |
author |
Chadzinska, M.K. Baginski, P. Kolaczkowska, E. Savelkoul, H.F.J. Verburg-van Kemenade, B.M.L. |
author_facet |
Chadzinska, M.K. Baginski, P. Kolaczkowska, E. Savelkoul, H.F.J. Verburg-van Kemenade, B.M.L. |
author_sort |
Chadzinska, M.K. |
title |
Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
title_short |
Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
title_full |
Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
title_fullStr |
Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
title_full_unstemmed |
Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
title_sort |
expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation |
publishDate |
2008 |
url |
https://research.wur.nl/en/publications/expression-profiles-of-matrix-metalloproteinase-9-in-teleost-fish https://doi.org/10.1111/j.1365-2567.2008.02874.x |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Immunology 125 (2008) 4 ISSN: 0019-2805 |
op_relation |
https://edepot.wur.nl/23993 https://research.wur.nl/en/publications/expression-profiles-of-matrix-metalloproteinase-9-in-teleost-fish doi:10.1111/j.1365-2567.2008.02874.x |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1111/j.1365-2567.2008.02874.x |
container_title |
Immunology |
container_volume |
125 |
container_issue |
4 |
container_start_page |
601 |
op_container_end_page |
610 |
_version_ |
1789965835945639936 |