Comparison of two-phase lipase-catalyzed esterification on micro and bench scale
Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1-butanol in a water/n-decane two-phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench...
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ftunivwagenin:oai:library.wur.nl:wurpubs/362933 2024-01-21T10:00:43+01:00 Comparison of two-phase lipase-catalyzed esterification on micro and bench scale Swarts, J.W. Vossenberg, P. Meerman, M.H. Janssen, A.E.M. Boom, R.M. 2008 application/pdf https://research.wur.nl/en/publications/comparison-of-two-phase-lipase-catalyzed-esterification-on-micro- https://doi.org/10.1002/bit.21650 en eng https://edepot.wur.nl/41307 https://research.wur.nl/en/publications/comparison-of-two-phase-lipase-catalyzed-esterification-on-micro- doi:10.1002/bit.21650 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Biotechnology and Bioengineering 99 (2008) 4 ISSN: 0006-3592 hexane immobilized lipase kinetics parameters info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2008 ftunivwagenin https://doi.org/10.1002/bit.21650 2023-12-27T23:15:38Z Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1-butanol in a water/n-decane two-phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling Article in Journal/Newspaper Antarc* Antarctica Wageningen UR (University & Research Centre): Digital Library Biotechnology and Bioengineering 99 4 855 861 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
hexane immobilized lipase kinetics parameters |
spellingShingle |
hexane immobilized lipase kinetics parameters Swarts, J.W. Vossenberg, P. Meerman, M.H. Janssen, A.E.M. Boom, R.M. Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
topic_facet |
hexane immobilized lipase kinetics parameters |
description |
Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1-butanol in a water/n-decane two-phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found. Furthermore, effects of temperature on activation and inactivation of the enzyme were found to be similar on micro and bench scale. Therefore, parameters found on either scale can be used for the other scale. Enzyme kinetic parameters can be determined on a micro scale, with very low consumption of reagents and catalyst, and then be applied to bench scale. This can reduce the cost of optimizing enzyme processes by downscaling |
format |
Article in Journal/Newspaper |
author |
Swarts, J.W. Vossenberg, P. Meerman, M.H. Janssen, A.E.M. Boom, R.M. |
author_facet |
Swarts, J.W. Vossenberg, P. Meerman, M.H. Janssen, A.E.M. Boom, R.M. |
author_sort |
Swarts, J.W. |
title |
Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
title_short |
Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
title_full |
Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
title_fullStr |
Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
title_full_unstemmed |
Comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
title_sort |
comparison of two-phase lipase-catalyzed esterification on micro and bench scale |
publishDate |
2008 |
url |
https://research.wur.nl/en/publications/comparison-of-two-phase-lipase-catalyzed-esterification-on-micro- https://doi.org/10.1002/bit.21650 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering 99 (2008) 4 ISSN: 0006-3592 |
op_relation |
https://edepot.wur.nl/41307 https://research.wur.nl/en/publications/comparison-of-two-phase-lipase-catalyzed-esterification-on-micro- doi:10.1002/bit.21650 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1002/bit.21650 |
container_title |
Biotechnology and Bioengineering |
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99 |
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4 |
container_start_page |
855 |
op_container_end_page |
861 |
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1788703462975864832 |