Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies
Integument biopsy is a nondestructive method for sampling free-ranging cetaceans, which allows for the determination of both contaminant concentrations and biomarker responses. Cytochrome P450 1A1 (CYP1A1) expression is induced by polycyclic aromatic hydrocarbons and planar halogenated aromatic hydr...
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ftunivwagenin:oai:library.wur.nl:wurpubs/357292 2024-01-14T10:05:39+01:00 Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies Wilson, J.Y. Wells, R. Anguilar, A. Borrell, A. Tornero, V. Reijnders, P.J.H. Moore, M. 2007 application/pdf https://research.wur.nl/en/publications/correlates-of-cytochrome-p450-1a1-expression-in-bottlenose-dolphi https://doi.org/10.1093/toxsci/kfm031 en eng https://edepot.wur.nl/32241 https://research.wur.nl/en/publications/correlates-of-cytochrome-p450-1a1-expression-in-bottlenose-dolphi doi:10.1093/toxsci/kfm031 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Toxicological sciences 97 (2007) 1 ISSN: 1096-6080 delphinapterus-leucas dibenzo-p-dioxins hepatocyte cultures organochlorine contaminants porpoise phocoena-phocoena sarasota bay skin biopsy st-lawrence toxic equivalency factors whales balaenoptera-physalus info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2007 ftunivwagenin https://doi.org/10.1093/toxsci/kfm031 2023-12-20T23:19:37Z Integument biopsy is a nondestructive method for sampling free-ranging cetaceans, which allows for the determination of both contaminant concentrations and biomarker responses. Cytochrome P450 1A1 (CYP1A1) expression is induced by polycyclic aromatic hydrocarbons and planar halogenated aromatic hydrocarbons such as the non-ortho and mono-ortho polychlorinated biphenyls (PCBs). CYP1A induction has been used extensively as a biomarker of exposure to such compounds in vertebrates. We measured PCB concentrations and CYP1A1 expression in integument biopsies from bottlenose dolphins (Tursiops truncatus) resident in Sarasota Bay, FL. This population of dolphins has been the subject of long-term population and health assessment, affording the opportunity to evaluate the influence of age, sex, and reproductive status on CYP1A1 expression. CYP1A1 expression was seen in endothelial cells, vascular smooth muscle, and nerve cells in the dermis, similar to what has been observed in other cetacean species. Endothelial CYP1A1 expression varied along the length of the biopsy, which could be related to differences in the structure and functionality of the blubber in different parts of the integument. Neither age nor sex was related to CYP1A1 expression in these biopsies, and reproductive status did not relate to levels of CYP1A1 in females. Total PCB and toxic equivalent quotient concentrations in blubber were positively correlated with dermal endothelial CYP1A1 expression, although mono-ortho PCBs concentrations did not show this relationship. Contaminant concentrations appear to be stronger determinants of CYP1A1 expression in integument of these dolphins, than are age, sex, or reproductive status. Article in Journal/Newspaper Balaenoptera physalus Delphinapterus leucas Phocoena phocoena Wageningen UR (University & Research Centre): Digital Library Toxicological Sciences 97 1 111 119 |
institution |
Open Polar |
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Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
delphinapterus-leucas dibenzo-p-dioxins hepatocyte cultures organochlorine contaminants porpoise phocoena-phocoena sarasota bay skin biopsy st-lawrence toxic equivalency factors whales balaenoptera-physalus |
spellingShingle |
delphinapterus-leucas dibenzo-p-dioxins hepatocyte cultures organochlorine contaminants porpoise phocoena-phocoena sarasota bay skin biopsy st-lawrence toxic equivalency factors whales balaenoptera-physalus Wilson, J.Y. Wells, R. Anguilar, A. Borrell, A. Tornero, V. Reijnders, P.J.H. Moore, M. Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
topic_facet |
delphinapterus-leucas dibenzo-p-dioxins hepatocyte cultures organochlorine contaminants porpoise phocoena-phocoena sarasota bay skin biopsy st-lawrence toxic equivalency factors whales balaenoptera-physalus |
description |
Integument biopsy is a nondestructive method for sampling free-ranging cetaceans, which allows for the determination of both contaminant concentrations and biomarker responses. Cytochrome P450 1A1 (CYP1A1) expression is induced by polycyclic aromatic hydrocarbons and planar halogenated aromatic hydrocarbons such as the non-ortho and mono-ortho polychlorinated biphenyls (PCBs). CYP1A induction has been used extensively as a biomarker of exposure to such compounds in vertebrates. We measured PCB concentrations and CYP1A1 expression in integument biopsies from bottlenose dolphins (Tursiops truncatus) resident in Sarasota Bay, FL. This population of dolphins has been the subject of long-term population and health assessment, affording the opportunity to evaluate the influence of age, sex, and reproductive status on CYP1A1 expression. CYP1A1 expression was seen in endothelial cells, vascular smooth muscle, and nerve cells in the dermis, similar to what has been observed in other cetacean species. Endothelial CYP1A1 expression varied along the length of the biopsy, which could be related to differences in the structure and functionality of the blubber in different parts of the integument. Neither age nor sex was related to CYP1A1 expression in these biopsies, and reproductive status did not relate to levels of CYP1A1 in females. Total PCB and toxic equivalent quotient concentrations in blubber were positively correlated with dermal endothelial CYP1A1 expression, although mono-ortho PCBs concentrations did not show this relationship. Contaminant concentrations appear to be stronger determinants of CYP1A1 expression in integument of these dolphins, than are age, sex, or reproductive status. |
format |
Article in Journal/Newspaper |
author |
Wilson, J.Y. Wells, R. Anguilar, A. Borrell, A. Tornero, V. Reijnders, P.J.H. Moore, M. |
author_facet |
Wilson, J.Y. Wells, R. Anguilar, A. Borrell, A. Tornero, V. Reijnders, P.J.H. Moore, M. |
author_sort |
Wilson, J.Y. |
title |
Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
title_short |
Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
title_full |
Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
title_fullStr |
Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
title_full_unstemmed |
Correlates of Cytochrome P450 1A1 Expression in Bottlenose Dolphin (Tursiops truncatus) Integument Biopsies |
title_sort |
correlates of cytochrome p450 1a1 expression in bottlenose dolphin (tursiops truncatus) integument biopsies |
publishDate |
2007 |
url |
https://research.wur.nl/en/publications/correlates-of-cytochrome-p450-1a1-expression-in-bottlenose-dolphi https://doi.org/10.1093/toxsci/kfm031 |
genre |
Balaenoptera physalus Delphinapterus leucas Phocoena phocoena |
genre_facet |
Balaenoptera physalus Delphinapterus leucas Phocoena phocoena |
op_source |
Toxicological sciences 97 (2007) 1 ISSN: 1096-6080 |
op_relation |
https://edepot.wur.nl/32241 https://research.wur.nl/en/publications/correlates-of-cytochrome-p450-1a1-expression-in-bottlenose-dolphi doi:10.1093/toxsci/kfm031 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1093/toxsci/kfm031 |
container_title |
Toxicological Sciences |
container_volume |
97 |
container_issue |
1 |
container_start_page |
111 |
op_container_end_page |
119 |
_version_ |
1788059989184610304 |