Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005
The present study was designed to identify 15 beta-hemolytic streptococci isolated during a period between 1988 and 2005 from nine harbour seals and six grey seals from various origins of the North Sea. All isolates were identified as Streptococcus equi subsp. zooepidemicus. The bacteria were additi...
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2007
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Online Access: | https://research.wur.nl/en/publications/relatedness-of-streptococcus-equi-subsp-zooepidemicus-strains-iso https://doi.org/10.1016/j.vetmic.2006.11.015 |
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ftunivwagenin:oai:library.wur.nl:wurpubs/355313 2024-02-04T10:03:55+01:00 Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 Akineden, Oe. Alber, J. Laemmler, C. Weiss, R. Siebert, U. Foster, G. Tougaard, S. Brasseur, S.M.J.M. Reijnders, P.J.H. 2007 application/pdf https://research.wur.nl/en/publications/relatedness-of-streptococcus-equi-subsp-zooepidemicus-strains-iso https://doi.org/10.1016/j.vetmic.2006.11.015 en eng https://edepot.wur.nl/35133 https://research.wur.nl/en/publications/relatedness-of-streptococcus-equi-subsp-zooepidemicus-strains-iso doi:10.1016/j.vetmic.2006.11.015 info:eu-repo/semantics/restrictedAccess Wageningen University & Research Veterinary Microbiology 121 (2007) 1-2 ISSN: 0378-1135 german north identification molecular characterization info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2007 ftunivwagenin https://doi.org/10.1016/j.vetmic.2006.11.015 2024-01-10T23:24:47Z The present study was designed to identify 15 beta-hemolytic streptococci isolated during a period between 1988 and 2005 from nine harbour seals and six grey seals from various origins of the North Sea. All isolates were identified as Streptococcus equi subsp. zooepidemicus. The bacteria were additionally investigated for relatedness by restriction fragment length polymorphism analysis of PCR amplified 16S-23S rDNA intergenic spacer region and gene szp and by macrorestriction analysis of chromosomal DNA of the strains by pulsed field gel electrophoresis. The molecular analysis yielded identical or closely related patterns within the strains of the present study and with the S. equi subsp. zooepidemicus strains isolated from harbour seals of German North Sea which were investigated previously [Akineden, O., Hassan, A.A., Alber, J., El-Sayed, A., Estoepangestie, A.T.S., Lammler, C., Weiss, R., Siebert, U., 2005. Phenotypic and genotypic properties of S. equi subsp. zooepidemicus isolated from harbor seals (Phoca vitulina) from the German North Sea during the phocine distemper outbreak in 2002. Vet. Microbiol. 110, 147-152]. This indicates that this single or closely related bacterial clone existed during both phocine distemper virus epidemics in 1988 and 2002 and that a direct transmission of the strains has occurred between two seal species and between seal populations of far distant regions possibly with grey seals as a vector. Article in Journal/Newspaper Phoca vitulina Wageningen UR (University & Research Centre): Digital Library Siebert ENVELOPE(-63.033,-63.033,-64.817,-64.817) Veterinary Microbiology 121 1-2 158 162 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
german north identification molecular characterization |
spellingShingle |
german north identification molecular characterization Akineden, Oe. Alber, J. Laemmler, C. Weiss, R. Siebert, U. Foster, G. Tougaard, S. Brasseur, S.M.J.M. Reijnders, P.J.H. Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
topic_facet |
german north identification molecular characterization |
description |
The present study was designed to identify 15 beta-hemolytic streptococci isolated during a period between 1988 and 2005 from nine harbour seals and six grey seals from various origins of the North Sea. All isolates were identified as Streptococcus equi subsp. zooepidemicus. The bacteria were additionally investigated for relatedness by restriction fragment length polymorphism analysis of PCR amplified 16S-23S rDNA intergenic spacer region and gene szp and by macrorestriction analysis of chromosomal DNA of the strains by pulsed field gel electrophoresis. The molecular analysis yielded identical or closely related patterns within the strains of the present study and with the S. equi subsp. zooepidemicus strains isolated from harbour seals of German North Sea which were investigated previously [Akineden, O., Hassan, A.A., Alber, J., El-Sayed, A., Estoepangestie, A.T.S., Lammler, C., Weiss, R., Siebert, U., 2005. Phenotypic and genotypic properties of S. equi subsp. zooepidemicus isolated from harbor seals (Phoca vitulina) from the German North Sea during the phocine distemper outbreak in 2002. Vet. Microbiol. 110, 147-152]. This indicates that this single or closely related bacterial clone existed during both phocine distemper virus epidemics in 1988 and 2002 and that a direct transmission of the strains has occurred between two seal species and between seal populations of far distant regions possibly with grey seals as a vector. |
format |
Article in Journal/Newspaper |
author |
Akineden, Oe. Alber, J. Laemmler, C. Weiss, R. Siebert, U. Foster, G. Tougaard, S. Brasseur, S.M.J.M. Reijnders, P.J.H. |
author_facet |
Akineden, Oe. Alber, J. Laemmler, C. Weiss, R. Siebert, U. Foster, G. Tougaard, S. Brasseur, S.M.J.M. Reijnders, P.J.H. |
author_sort |
Akineden, Oe. |
title |
Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
title_short |
Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
title_full |
Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
title_fullStr |
Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
title_full_unstemmed |
Relatedness of Streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) of various origins of the North Sea during 1988 - 2005 |
title_sort |
relatedness of streptococcus equi subsp. zooepidemicus strains isolated from harbour seals (phoca vitulina) and grey seals (halichoerus grypus) of various origins of the north sea during 1988 - 2005 |
publishDate |
2007 |
url |
https://research.wur.nl/en/publications/relatedness-of-streptococcus-equi-subsp-zooepidemicus-strains-iso https://doi.org/10.1016/j.vetmic.2006.11.015 |
long_lat |
ENVELOPE(-63.033,-63.033,-64.817,-64.817) |
geographic |
Siebert |
geographic_facet |
Siebert |
genre |
Phoca vitulina |
genre_facet |
Phoca vitulina |
op_source |
Veterinary Microbiology 121 (2007) 1-2 ISSN: 0378-1135 |
op_relation |
https://edepot.wur.nl/35133 https://research.wur.nl/en/publications/relatedness-of-streptococcus-equi-subsp-zooepidemicus-strains-iso doi:10.1016/j.vetmic.2006.11.015 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1016/j.vetmic.2006.11.015 |
container_title |
Veterinary Microbiology |
container_volume |
121 |
container_issue |
1-2 |
container_start_page |
158 |
op_container_end_page |
162 |
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1789971757432569856 |