Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum
Four strains of thermophilic, endospore-forming, sulfate-reducing bacteria were enriched and isolated from hot solfataric fields in the Krafla area of north-east Iceland, using methanol and sulfite as substrates. Morphologically, these strains resembled thermophilic Desulfotomaculum species. The str...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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ftunivwagenin:oai:library.wur.nl:wurpubs/326438 2024-01-21T10:07:23+01:00 Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum Goorissen, H.P. Stams, A.J.M. Hansen, T.A. 2003 application/pdf https://research.wur.nl/en/publications/isolation-of-thermophilic-desulfotomaculum-strains-with-methanol- https://doi.org/10.1099/ijs.0.02476-0 en eng https://edepot.wur.nl/374305 https://research.wur.nl/en/publications/isolation-of-thermophilic-desulfotomaculum-strains-with-methanol- doi:10.1099/ijs.0.02476-0 info:eu-repo/semantics/restrictedAccess Wageningen University & Research International Journal of Systematic and Evolutionary Microbiology 53 (2003) ISSN: 1466-5026 anaerobic bacterium deep gen-nov marine sediment oil-field waters reductive dechlorination reservoir sulfate-reducing bacterium tetrachloroethene thermodesulfobacterium-commune info:eu-repo/semantics/article Article/Letter to editor info:eu-repo/semantics/publishedVersion 2003 ftunivwagenin https://doi.org/10.1099/ijs.0.02476-0 2023-12-27T23:16:16Z Four strains of thermophilic, endospore-forming, sulfate-reducing bacteria were enriched and isolated from hot solfataric fields in the Krafla area of north-east Iceland, using methanol and sulfite as substrates. Morphologically, these strains resembled thermophilic Desulfotomaculum species. The strains grew with alcohols, including methanol, with glucose and fructose as electron donors, and with sulfate, sulfite or thiosulfate as electron acceptors. For all four strains, the optimum temperature and pH for growth were 60 degreesC and pH 7.3, respectively; no added NaCl was required. Phylogenetic analysis based on partial 16S rRNA gene sequence comparisons showed high levels of similarity of the novel strains (> 92 %) with Desulfotomaculum kuznetsovii and Desulfotomaculum luciae. However, DNA-DNA hybridization studies with D. kuznetsovii revealed that the four strains belonged to one novel species. A representative of this group of isolates, strain V21(T), is proposed as the type strain of a novel species of the spore-forming, sulfate-reducing genus Desulfotomaculum, namely Desulfotomaculum solfataricum (type strain V21(T) = DSM 114956(T) = CIP 107984(T)). Article in Journal/Newspaper Iceland Wageningen UR (University & Research Centre): Digital Library Krafla ENVELOPE(-16.747,-16.747,65.713,65.713) International Journal of Systematic and Evolutionary Microbiology 53 5 1223 1229 |
institution |
Open Polar |
collection |
Wageningen UR (University & Research Centre): Digital Library |
op_collection_id |
ftunivwagenin |
language |
English |
topic |
anaerobic bacterium deep gen-nov marine sediment oil-field waters reductive dechlorination reservoir sulfate-reducing bacterium tetrachloroethene thermodesulfobacterium-commune |
spellingShingle |
anaerobic bacterium deep gen-nov marine sediment oil-field waters reductive dechlorination reservoir sulfate-reducing bacterium tetrachloroethene thermodesulfobacterium-commune Goorissen, H.P. Stams, A.J.M. Hansen, T.A. Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
topic_facet |
anaerobic bacterium deep gen-nov marine sediment oil-field waters reductive dechlorination reservoir sulfate-reducing bacterium tetrachloroethene thermodesulfobacterium-commune |
description |
Four strains of thermophilic, endospore-forming, sulfate-reducing bacteria were enriched and isolated from hot solfataric fields in the Krafla area of north-east Iceland, using methanol and sulfite as substrates. Morphologically, these strains resembled thermophilic Desulfotomaculum species. The strains grew with alcohols, including methanol, with glucose and fructose as electron donors, and with sulfate, sulfite or thiosulfate as electron acceptors. For all four strains, the optimum temperature and pH for growth were 60 degreesC and pH 7.3, respectively; no added NaCl was required. Phylogenetic analysis based on partial 16S rRNA gene sequence comparisons showed high levels of similarity of the novel strains (> 92 %) with Desulfotomaculum kuznetsovii and Desulfotomaculum luciae. However, DNA-DNA hybridization studies with D. kuznetsovii revealed that the four strains belonged to one novel species. A representative of this group of isolates, strain V21(T), is proposed as the type strain of a novel species of the spore-forming, sulfate-reducing genus Desulfotomaculum, namely Desulfotomaculum solfataricum (type strain V21(T) = DSM 114956(T) = CIP 107984(T)). |
format |
Article in Journal/Newspaper |
author |
Goorissen, H.P. Stams, A.J.M. Hansen, T.A. |
author_facet |
Goorissen, H.P. Stams, A.J.M. Hansen, T.A. |
author_sort |
Goorissen, H.P. |
title |
Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
title_short |
Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
title_full |
Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
title_fullStr |
Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
title_full_unstemmed |
Isolation of thermophilic Desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum |
title_sort |
isolation of thermophilic desulfotomaculum strains with methanol and sulphite from solfataric mud pools, and characterization of desulfotomaculum solfataricum |
publishDate |
2003 |
url |
https://research.wur.nl/en/publications/isolation-of-thermophilic-desulfotomaculum-strains-with-methanol- https://doi.org/10.1099/ijs.0.02476-0 |
long_lat |
ENVELOPE(-16.747,-16.747,65.713,65.713) |
geographic |
Krafla |
geographic_facet |
Krafla |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
International Journal of Systematic and Evolutionary Microbiology 53 (2003) ISSN: 1466-5026 |
op_relation |
https://edepot.wur.nl/374305 https://research.wur.nl/en/publications/isolation-of-thermophilic-desulfotomaculum-strains-with-methanol- doi:10.1099/ijs.0.02476-0 |
op_rights |
info:eu-repo/semantics/restrictedAccess Wageningen University & Research |
op_doi |
https://doi.org/10.1099/ijs.0.02476-0 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
53 |
container_issue |
5 |
container_start_page |
1223 |
op_container_end_page |
1229 |
_version_ |
1788697950212325376 |