Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish
Alike other flat fish, marine turbot has the particularity that changes from larvae with bilateral symmetry to adult with asymmetry, in terms of the position of the eyes. As expected, the skin configuration of this species is also affected by the development and transformation suffered by fish durin...
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Journal of Fish Biology
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ftunivvigo:oai:www.investigo.biblioteca.uvigo.es:11093/4629 2023-05-15T18:15:53+02:00 Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish Landeira Dabarca, Andrea Abreu, Cristina S. R. Alvarez Jimenez, Maruxa Molist García, Maria Del Pilar 2021-12 http://hdl.handle.net/11093/4629 https://doi.org/10.1111/jfb.14910 https://onlinelibrary.wiley.com/doi/10.1111/jfb.14910 eng eng Journal of Fish Biology Ecoloxía e bioloxía animal Ecoloxía Evolutiva info:eu-repo/grantAgreement/MICINN//CGL2009-07904/ES/Mensajes Quimicos Producidos Por Salmonidos Y Mecanismos De Defensa Anti-Depredador Journal of Fish Biology, 99(6): 2018-2029 (2021) 00221112 10958649 http://hdl.handle.net/11093/4629 doi:10.1111/jfb.14910 https://onlinelibrary.wiley.com/doi/10.1111/jfb.14910 Attribution-NonCommercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/ openAccess 2407.03 Morfología Celular article 2021 ftunivvigo https://doi.org/10.1111/jfb.14910 2023-04-11T23:23:44Z Alike other flat fish, marine turbot has the particularity that changes from larvae with bilateral symmetry to adult with asymmetry, in terms of the position of the eyes. As expected, the skin configuration of this species is also affected by the development and transformation suffered by fish during metamorphosis. In this context, changes in the epidermis of marine turbot were studied using conventional staining and histochemical techniques using six lectins (UEA-I, PNA, RCA-I, WGA, Con A and SBA). During development from larvae to juvenile (3–300 days post-hatching), the epidermis increased in both thickness and the number of cell layers. In fact, the simple cuboidal epithelium observed in larvae at day 3 already became stratified at days 10–12, which sequentially increase in thickness with fish development. Turbot epidermis is composed basically of four cell types: epithelial and mucous or secretory cells that are present through the development, and pigmented cells and a type that the authors described as club-like cells that appear during and post-metamorphosis. The Alcian blue-periodic acid Schiff (AB-PAS) histochemical method revealed the presence of neutral glycoconjugates in mucous and club-like cells at post-metamorphic stages of fish. Accordingly, lectin analysis showed mucous cells containing glycoproteins rich in fucose (UEA-I labelling) and glycoconjugates rich in the sequence galactose-N-acetyl galactosamine (PNA and RCA-I labelling) when this cell type appears. Interestingly, melanophores were observed in the dorsal epidermis of post-metamorphic juveniles. This type of cell contains a black-to-brown pigment that provides the skin the typical colour of this fish species. Changes in mucous coat composition were observed during fish development, which was attributed to different roles of the glycoconjugates. Ministerio de Ciencia e Innovación | Ref. CGL 2009-07904 Universidade de Vigo/ CISUG Article in Journal/Newspaper Scophthalmus maximus Turbot University of Vigo: Investigo (Repositorio Institucional de la Universidade de Vigo) Journal of Fish Biology 99 6 2018 2029 |
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Open Polar |
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University of Vigo: Investigo (Repositorio Institucional de la Universidade de Vigo) |
op_collection_id |
ftunivvigo |
language |
English |
topic |
2407.03 Morfología Celular |
spellingShingle |
2407.03 Morfología Celular Landeira Dabarca, Andrea Abreu, Cristina S. R. Alvarez Jimenez, Maruxa Molist García, Maria Del Pilar Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
topic_facet |
2407.03 Morfología Celular |
description |
Alike other flat fish, marine turbot has the particularity that changes from larvae with bilateral symmetry to adult with asymmetry, in terms of the position of the eyes. As expected, the skin configuration of this species is also affected by the development and transformation suffered by fish during metamorphosis. In this context, changes in the epidermis of marine turbot were studied using conventional staining and histochemical techniques using six lectins (UEA-I, PNA, RCA-I, WGA, Con A and SBA). During development from larvae to juvenile (3–300 days post-hatching), the epidermis increased in both thickness and the number of cell layers. In fact, the simple cuboidal epithelium observed in larvae at day 3 already became stratified at days 10–12, which sequentially increase in thickness with fish development. Turbot epidermis is composed basically of four cell types: epithelial and mucous or secretory cells that are present through the development, and pigmented cells and a type that the authors described as club-like cells that appear during and post-metamorphosis. The Alcian blue-periodic acid Schiff (AB-PAS) histochemical method revealed the presence of neutral glycoconjugates in mucous and club-like cells at post-metamorphic stages of fish. Accordingly, lectin analysis showed mucous cells containing glycoproteins rich in fucose (UEA-I labelling) and glycoconjugates rich in the sequence galactose-N-acetyl galactosamine (PNA and RCA-I labelling) when this cell type appears. Interestingly, melanophores were observed in the dorsal epidermis of post-metamorphic juveniles. This type of cell contains a black-to-brown pigment that provides the skin the typical colour of this fish species. Changes in mucous coat composition were observed during fish development, which was attributed to different roles of the glycoconjugates. Ministerio de Ciencia e Innovación | Ref. CGL 2009-07904 Universidade de Vigo/ CISUG |
format |
Article in Journal/Newspaper |
author |
Landeira Dabarca, Andrea Abreu, Cristina S. R. Alvarez Jimenez, Maruxa Molist García, Maria Del Pilar |
author_facet |
Landeira Dabarca, Andrea Abreu, Cristina S. R. Alvarez Jimenez, Maruxa Molist García, Maria Del Pilar |
author_sort |
Landeira Dabarca, Andrea |
title |
Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
title_short |
Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
title_full |
Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
title_fullStr |
Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
title_full_unstemmed |
Changes in marine turbot (Scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
title_sort |
changes in marine turbot (scophthalmus maximus) epidermis and skin mucus composition during development from bilateral larvae to juvenile flat fish |
publisher |
Journal of Fish Biology |
publishDate |
2021 |
url |
http://hdl.handle.net/11093/4629 https://doi.org/10.1111/jfb.14910 https://onlinelibrary.wiley.com/doi/10.1111/jfb.14910 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
info:eu-repo/grantAgreement/MICINN//CGL2009-07904/ES/Mensajes Quimicos Producidos Por Salmonidos Y Mecanismos De Defensa Anti-Depredador Journal of Fish Biology, 99(6): 2018-2029 (2021) 00221112 10958649 http://hdl.handle.net/11093/4629 doi:10.1111/jfb.14910 https://onlinelibrary.wiley.com/doi/10.1111/jfb.14910 |
op_rights |
Attribution-NonCommercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/ openAccess |
op_doi |
https://doi.org/10.1111/jfb.14910 |
container_title |
Journal of Fish Biology |
container_volume |
99 |
container_issue |
6 |
container_start_page |
2018 |
op_container_end_page |
2029 |
_version_ |
1766189141867888640 |