Salting out Tergitol 15S-based surfactants for extremolipases separation

• Published in: Journal of Molecular Liquids
• Main Authors: Gonzalez Cabaleiro, Laura, Deive Herva, Francisco Javier, Rodríguez Rodríguez, Ana María, Longo González, María Asunción, Álvarez Álvarez, María Salomé
• Format: Article in Journal/Newspaper
• Language: English
• Published: Journal of Molecular Liquids 2022
• Subjects: 3310.05 Ingeniería de Procesos; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11093/3381; https://doi.org/10.1016/j.molliq.2022.118736; https://linkinghub.elsevier.com/retrieve/pii/S0167732222002732

In the present work, the ability of different sodium-based salts like Na2CO3, Na2HPO4, Na2SO4 and Na2SO3 to salt out aqueous solutions of two biodegradable non-ionic surfactants (Tergitol 15S7 and Tergitol 15S9) has been demonstrated. We have empirically characterized the immiscibility regions and models like Merchuk and Othmer-Tobias equations were employed for binodal and tie-line data, respectively. The effect of phase forming components on the lipolytic activity of two in-house extremolipases (from Thermus thermophilus HB27 and Halomonas sp-LM1C) was studied prior to approach the separation. It was concluded that ions bearing the lowest capacity to establish hydrogen bonds with water molecules like sulphate led to greater activation effect. Then, the ability of the proposed systems to extract both lipases was compared with a commercial one, lipase B from Candida antarctica, and it was concluded that 50% of activity decay was avoided when in-house extreme enzymes were employed. Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG Xunta de Galicia | Ref. ED481D-2019/017 Agencia Estatal de Investigación | Ref. RTI2018-094702-B-I00