A novel lipase-catalyzed method for preparing ELR-based bioconjugates

• Published in: International Journal of Biological Macromolecules
• Main Authors: Testera Gorgojo, Ana María, Santos García, María Mercedes, Girotti, Alessandra, Arias Vallejo, Francisco Javier, Báñez Sanz, José Manuel, Alonso Rodrigo, Matilde, Rodríguez Cabello, José Carlos
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2019
• Subjects: Elastina; Modificación enzimática; Elastine; Enzymatic modification; Antarc*; Antarctica
• Online Access: http://uvadoc.uva.es/handle/10324/33510; https://doi.org/10.1016/j.ijbiomac.2018.10.028

Producción Científica Herein we present a novel one-pot method for the chemical modification of elastin-like recombinamers (ELRs) in a mild and efficient manner involving enzymatic catalysis with Candida antarctica lipase B. The introduction of different functionalities into such ELRs could open up new possibilities for the development of advanced biomaterials for regenerative medicine and, specifically, for controlled drug delivery given their additional ability to respond to stimuli other than pH or temperature, such as glucose concentration or electromagnetic radiation. Candida antarctica lipase B immobilized on a macroporous acrylic resin (Novozym 435) was used to enzymatically couple different aminated substrates to a recombinamer containing carboxylic groups along its amino acid chain by way of an amidation reaction. A preliminary study of the kinetics of this amidation in response to different reaction conditions, such as solvent, temperature or reagent ratio, was carried out using a phenylazobenzene derivative (azo-NH2) as a model. The optimal amidation conditions were used to couple other amine reagents, such as phenylboronic acid (FB-NH2) or polyethylene glycol (PEG-NH2), thus allowing us to obtain photoresponsive, glucose-responsive or PEGylated ELRs that could potentially be useful as sensors in devices for controlled drug delivery. 2019-11-11 2019-11-11 European Social Fund (ESF) and European Regional Development Fund (ERDF) Comisión Europea (proyectos NMP-2014-646075, HEALTH-F4-2011-278557, PITN-GA-2012-317306 y MSCA-ITN-2014-642687), Ministerio de Economía, Industria y Competitividad (Projects MAT2015-68901-R, MAT2016-79435-R and MAT2016-78903-R) Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13 y VA313U14) CIBER-BBN, Instituto de Salud Carlos III a través de la Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Novozymes®