Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence.
The current study aims to assess contaminant levels and tissue burdens in hooded seal (Cystophora cristata) blubber, liver, and blood in association with cytochrome P450 (CYP) enzymes (CYP1A and -3A) and serum analytes (hepatic enzymes like alanine aminotransferase [ALT], aspartate aminotransferase,...
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2009
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ftunivutrecht:oai:dspace.library.uu.nl:1874/39413 2023-07-23T04:18:58+02:00 Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. Wolkers, H. Boily, F. Fink-Gremmels, J. Bavel, B. Hammill, M.O. Primicerio, R. Risk Assessment of Toxic and Immunomodulatory Agents Dep IRAS 2009 application/pdf https://dspace.library.uu.nl/handle/1874/39413 other unknown 0090-4341 https://dspace.library.uu.nl/handle/1874/39413 info:eu-repo/semantics/EmbargoedAccess Article 2009 ftunivutrecht 2023-07-01T23:37:54Z The current study aims to assess contaminant levels and tissue burdens in hooded seal (Cystophora cristata) blubber, liver, and blood in association with cytochrome P450 (CYP) enzymes (CYP1A and -3A) and serum analytes (hepatic enzymes like alanine aminotransferase [ALT], aspartate aminotransferase, alkaline phosphatase [AP], and gamma-glutamyltransferase [GGT], serum proteins, and creatine kinase). Contaminant accumulation levels and patterns of polychlorinated biphenyls, chlorinated pesticides, and polybrominated diphenyl ethers (PBDEs) differed between tissues and seal groups, with the highest levels in liver. Pups showed higher liver contaminant levels, especially for PBDEs, than adults. These high levels might be associated with the ingestion of large amounts of contaminated milk and subsequent accumulation in the liver. Adult males and females mainly differed in PBDE levels, which were higher in females, possibly due to a sex-specific diet. The association between blubber contaminant burdens and the diagnostic enzymes ALT, GGT, and AP, and serum albumin, was inconclusive. In contrast, several CYP isoenzymes showed a clear positive relationship with the overall blubber contaminant burden, indicating enzyme induction following exposure to polyhalogenated hydrocarbons. Therefore, liver CYP isoenzymes may serve as a sensitive biomarker for long-term exposure to polyhalogenated hydrocarbons. Article in Journal/Newspaper Cystophora cristata hooded seal Utrecht University Repository |
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Utrecht University Repository |
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description |
The current study aims to assess contaminant levels and tissue burdens in hooded seal (Cystophora cristata) blubber, liver, and blood in association with cytochrome P450 (CYP) enzymes (CYP1A and -3A) and serum analytes (hepatic enzymes like alanine aminotransferase [ALT], aspartate aminotransferase, alkaline phosphatase [AP], and gamma-glutamyltransferase [GGT], serum proteins, and creatine kinase). Contaminant accumulation levels and patterns of polychlorinated biphenyls, chlorinated pesticides, and polybrominated diphenyl ethers (PBDEs) differed between tissues and seal groups, with the highest levels in liver. Pups showed higher liver contaminant levels, especially for PBDEs, than adults. These high levels might be associated with the ingestion of large amounts of contaminated milk and subsequent accumulation in the liver. Adult males and females mainly differed in PBDE levels, which were higher in females, possibly due to a sex-specific diet. The association between blubber contaminant burdens and the diagnostic enzymes ALT, GGT, and AP, and serum albumin, was inconclusive. In contrast, several CYP isoenzymes showed a clear positive relationship with the overall blubber contaminant burden, indicating enzyme induction following exposure to polyhalogenated hydrocarbons. Therefore, liver CYP isoenzymes may serve as a sensitive biomarker for long-term exposure to polyhalogenated hydrocarbons. |
author2 |
Risk Assessment of Toxic and Immunomodulatory Agents Dep IRAS |
format |
Article in Journal/Newspaper |
author |
Wolkers, H. Boily, F. Fink-Gremmels, J. Bavel, B. Hammill, M.O. Primicerio, R. |
spellingShingle |
Wolkers, H. Boily, F. Fink-Gremmels, J. Bavel, B. Hammill, M.O. Primicerio, R. Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
author_facet |
Wolkers, H. Boily, F. Fink-Gremmels, J. Bavel, B. Hammill, M.O. Primicerio, R. |
author_sort |
Wolkers, H. |
title |
Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
title_short |
Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
title_full |
Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
title_fullStr |
Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
title_full_unstemmed |
Tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome P450 enzyme activities in hooded seals (Cystophora cristata) from the Gulf of St. Lawrence. |
title_sort |
tissue-specific contaminant accumulation and associated effects on hepatic serum analytes and cytochrome p450 enzyme activities in hooded seals (cystophora cristata) from the gulf of st. lawrence. |
publishDate |
2009 |
url |
https://dspace.library.uu.nl/handle/1874/39413 |
genre |
Cystophora cristata hooded seal |
genre_facet |
Cystophora cristata hooded seal |
op_relation |
0090-4341 https://dspace.library.uu.nl/handle/1874/39413 |
op_rights |
info:eu-repo/semantics/EmbargoedAccess |
_version_ |
1772181707865194496 |