Sex steroid production associated with puberty is absent in germ cell-free salmon
In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ ce...
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ftunivutrecht:oai:dspace.library.uu.nl:1874/359012 2023-11-12T04:14:41+01:00 Sex steroid production associated with puberty is absent in germ cell-free salmon Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W Wargelius, Anna Sub Developmental Biology Developmental Biology 2017 image/pdf https://dspace.library.uu.nl/handle/1874/359012 en eng 2045-2322 https://dspace.library.uu.nl/handle/1874/359012 info:eu-repo/semantics/OpenAccess Gene expression Reproductive biology Article 2017 ftunivutrecht 2023-11-01T23:15:07Z In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ cell-free (GCF), dnd knockout and wild type (WT) postsmolts were stimulated to enter puberty. No GCF fish entered puberty, whereas 66.7% (males) and 30% (females) WT fish completed or entered puberty, respectively. Expression of genes related to steroidogenesis (star, cyp17a1, cyp11β, cyp19a1a), gonadal somatic cells (insl3, amh, igf3), oocytes (bmp15), gonadotropin receptors (fshr, lhcgr), and pituitary gonadotropic cells (fshb, lhb, gnrhr4) showed an immature status and failure to up-regulate gonadal sex steroid production in male and female GCF fish was also reflected in low or undetectable plasma sex steroids (11-ketotestosterone, estradiol-17β and testosterone). A gender difference (high in females, low in males) was found in the expression of star and cyp17a1 in GCF fish. No clear difference in growth was detected between GCF and immature WT fish, while growth was compromised in maturing WT males. We demonstrate for the first time in a vertebrate that germ cells are required for pubertal activation of the somatic steroidogenic cells. Article in Journal/Newspaper Atlantic salmon Utrecht University Repository |
institution |
Open Polar |
collection |
Utrecht University Repository |
op_collection_id |
ftunivutrecht |
language |
English |
topic |
Gene expression Reproductive biology |
spellingShingle |
Gene expression Reproductive biology Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W Wargelius, Anna Sex steroid production associated with puberty is absent in germ cell-free salmon |
topic_facet |
Gene expression Reproductive biology |
description |
In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ cell-free (GCF), dnd knockout and wild type (WT) postsmolts were stimulated to enter puberty. No GCF fish entered puberty, whereas 66.7% (males) and 30% (females) WT fish completed or entered puberty, respectively. Expression of genes related to steroidogenesis (star, cyp17a1, cyp11β, cyp19a1a), gonadal somatic cells (insl3, amh, igf3), oocytes (bmp15), gonadotropin receptors (fshr, lhcgr), and pituitary gonadotropic cells (fshb, lhb, gnrhr4) showed an immature status and failure to up-regulate gonadal sex steroid production in male and female GCF fish was also reflected in low or undetectable plasma sex steroids (11-ketotestosterone, estradiol-17β and testosterone). A gender difference (high in females, low in males) was found in the expression of star and cyp17a1 in GCF fish. No clear difference in growth was detected between GCF and immature WT fish, while growth was compromised in maturing WT males. We demonstrate for the first time in a vertebrate that germ cells are required for pubertal activation of the somatic steroidogenic cells. |
author2 |
Sub Developmental Biology Developmental Biology |
format |
Article in Journal/Newspaper |
author |
Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W Wargelius, Anna |
author_facet |
Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_short |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_full |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_fullStr |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_full_unstemmed |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_sort |
sex steroid production associated with puberty is absent in germ cell-free salmon |
publishDate |
2017 |
url |
https://dspace.library.uu.nl/handle/1874/359012 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
2045-2322 https://dspace.library.uu.nl/handle/1874/359012 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1782332275412697088 |