Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast
The presence of Brucella (B.) spp. in harbour porpoises stranded between 2008 and 2011 along the Dutch coast was studied. A selection of 265 tissue samples from 112 animals was analysed using conventional and molecular methods. In total, 4.5% (5/112) of the animals corresponding with 2.3% (6/265) Br...
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ftunivutrecht:oai:dspace.library.uu.nl:1874/309191 2023-07-23T04:21:24+02:00 Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast Maio, Elisa Begeman, Lineke Bisselink, Yvette van Tulden, Peter Wiersma, Lidewij Hiemstra, Sjoukje Ruuls, Robin Gröne, Andrea Roest, Hendrik-Ido-Jan Willemsen, Peter van der Giessen, Joke Veterinair Pathologisch Diagnostisch Cnt LS Pathologie PB AVM Advances in Veterinary Medicine 2014-09-17 image/pdf https://dspace.library.uu.nl/handle/1874/309191 en eng 0378-1135 https://dspace.library.uu.nl/handle/1874/309191 info:eu-repo/semantics/OpenAccess Animals Brucella Brucellosis Female Genome Bacterial Genotype Male Minisatellite Repeats Multilocus Sequence Typing Netherlands North Sea Phocoena Phylogeny Prevalence Real-Time Polymerase Chain Reaction Article 2014 ftunivutrecht 2023-07-02T01:15:42Z The presence of Brucella (B.) spp. in harbour porpoises stranded between 2008 and 2011 along the Dutch coast was studied. A selection of 265 tissue samples from 112 animals was analysed using conventional and molecular methods. In total, 4.5% (5/112) of the animals corresponding with 2.3% (6/265) Brucella positive tissue samples were Brucella positive by culture and these were all confirmed by real-time polymerase chain reaction (real-time PCR) based on the insertion element 711 (IS711). In addition, two more Brucella-positive tissue samples from two animals collected in 2011 were identified using real-time PCR resulting in an overall Brucella prevalence of 6.3% (7/112 animals). Brucella spp. were obtained from lungs (n=3), pulmonary lymph node (n=3) and lungworms (n=2). Multi Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) typing based on the MLVA-16 showed that the Brucella isolates were B. ceti. Additional in silico Multi Locus Sequence typing (MLST) after whole genome sequencing of the 6 Brucella isolates confirmed B. ceti ST 23. According to the Brucella 2010 MLVA database, the isolated Brucella strains encountered were of five genotypes, in two distinct subclusters divided in two different time periods of harbour porpoises collection. This study is the first population based analyses for Brucella spp. infections in cetaceans stranded along the Dutch coast. Article in Journal/Newspaper Phocoena phocoena Utrecht University Repository |
institution |
Open Polar |
collection |
Utrecht University Repository |
op_collection_id |
ftunivutrecht |
language |
English |
topic |
Animals Brucella Brucellosis Female Genome Bacterial Genotype Male Minisatellite Repeats Multilocus Sequence Typing Netherlands North Sea Phocoena Phylogeny Prevalence Real-Time Polymerase Chain Reaction |
spellingShingle |
Animals Brucella Brucellosis Female Genome Bacterial Genotype Male Minisatellite Repeats Multilocus Sequence Typing Netherlands North Sea Phocoena Phylogeny Prevalence Real-Time Polymerase Chain Reaction Maio, Elisa Begeman, Lineke Bisselink, Yvette van Tulden, Peter Wiersma, Lidewij Hiemstra, Sjoukje Ruuls, Robin Gröne, Andrea Roest, Hendrik-Ido-Jan Willemsen, Peter van der Giessen, Joke Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
topic_facet |
Animals Brucella Brucellosis Female Genome Bacterial Genotype Male Minisatellite Repeats Multilocus Sequence Typing Netherlands North Sea Phocoena Phylogeny Prevalence Real-Time Polymerase Chain Reaction |
description |
The presence of Brucella (B.) spp. in harbour porpoises stranded between 2008 and 2011 along the Dutch coast was studied. A selection of 265 tissue samples from 112 animals was analysed using conventional and molecular methods. In total, 4.5% (5/112) of the animals corresponding with 2.3% (6/265) Brucella positive tissue samples were Brucella positive by culture and these were all confirmed by real-time polymerase chain reaction (real-time PCR) based on the insertion element 711 (IS711). In addition, two more Brucella-positive tissue samples from two animals collected in 2011 were identified using real-time PCR resulting in an overall Brucella prevalence of 6.3% (7/112 animals). Brucella spp. were obtained from lungs (n=3), pulmonary lymph node (n=3) and lungworms (n=2). Multi Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) typing based on the MLVA-16 showed that the Brucella isolates were B. ceti. Additional in silico Multi Locus Sequence typing (MLST) after whole genome sequencing of the 6 Brucella isolates confirmed B. ceti ST 23. According to the Brucella 2010 MLVA database, the isolated Brucella strains encountered were of five genotypes, in two distinct subclusters divided in two different time periods of harbour porpoises collection. This study is the first population based analyses for Brucella spp. infections in cetaceans stranded along the Dutch coast. |
author2 |
Veterinair Pathologisch Diagnostisch Cnt LS Pathologie PB AVM Advances in Veterinary Medicine |
format |
Article in Journal/Newspaper |
author |
Maio, Elisa Begeman, Lineke Bisselink, Yvette van Tulden, Peter Wiersma, Lidewij Hiemstra, Sjoukje Ruuls, Robin Gröne, Andrea Roest, Hendrik-Ido-Jan Willemsen, Peter van der Giessen, Joke |
author_facet |
Maio, Elisa Begeman, Lineke Bisselink, Yvette van Tulden, Peter Wiersma, Lidewij Hiemstra, Sjoukje Ruuls, Robin Gröne, Andrea Roest, Hendrik-Ido-Jan Willemsen, Peter van der Giessen, Joke |
author_sort |
Maio, Elisa |
title |
Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
title_short |
Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
title_full |
Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
title_fullStr |
Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
title_full_unstemmed |
Identification and typing of Brucella spp. in stranded harbour porpoises (Phocoena phocoena) on the Dutch coast |
title_sort |
identification and typing of brucella spp. in stranded harbour porpoises (phocoena phocoena) on the dutch coast |
publishDate |
2014 |
url |
https://dspace.library.uu.nl/handle/1874/309191 |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_relation |
0378-1135 https://dspace.library.uu.nl/handle/1874/309191 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1772186927370338304 |