Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations
Perfluoroalkyl substances (PFASs) are widely used in a variety of industrial processes and products, and have been detected globally in humans and wildlife. PFASs are suspected to interfere with endocrine signaling and to adversely affect human reproductive health. The aim of the present study was t...
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ftunivutrecht:oai:dspace.library.uu.nl:1874/305607 2023-07-23T04:17:57+02:00 Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations Leter, Giorgio Consales, Claudia Eleuteri, Patrizia Uccelli, Raffaella Specht, Ina O. Toft, Gunnar Moccia, Tania Budillon, Alfredo Jönsson, Bo A G Lindh, Christian H. Giwercman, Aleksander Pedersen, Henning S. Ludwicki, Jan K. Zviezdai, Valentyna Heederik, Dick Bonde, Jens Peter E Spanò, Marcello LS IRAS EEPI GRA (Gezh.risico-analyse) Risk Assessment of Toxic and Immunomodulatory Agents IRAS RATIA-SIB 2014-01-01 image/pdf https://dspace.library.uu.nl/handle/1874/305607 en eng 0893-6692 https://dspace.library.uu.nl/handle/1874/305607 info:eu-repo/semantics/OpenAccess DNA methylation Epidemiology Flow cytometry Human spermatozoa Perfluoroalkyl substances Pyrosequencing Coronacrisis-Taverne Health Toxicology and Mutagenesis Genetics(clinical) Article 2014 ftunivutrecht 2023-07-02T01:09:52Z Perfluoroalkyl substances (PFASs) are widely used in a variety of industrial processes and products, and have been detected globally in humans and wildlife. PFASs are suspected to interfere with endocrine signaling and to adversely affect human reproductive health. The aim of the present study was to investigate the associations between exposure to PFASs and sperm global methylation levels in a population of non-occupationally exposed fertile men. Measurements of PFASs in serum from 262 partners of pregnant women from Greenland, Poland and Ukraine, were also carried out by liquid chromatography tandem mass spectrometry. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA) were detected in 97% of the blood samples. Two surrogate markers were used to assess DNA global methylation levels in semen samples from the same men: (a) average DNA methylation level in repetitive DNA sequences (Alu, LINE-1, Satα) quantified by PCR-pyrosequencing after bisulfite conversion; (b) flow cytometric immunodetection of 5-methyl-cytosines. After multivariate linear regression analysis, no major consistent associations between PFASs exposure and sperm DNA global methylation endpoints could be detected. However, since weak but statistically significant associations of different PFASs with DNA hypo- and hyper-methylation were found in some of the studied populations, effects of PFASs on sperm epigenetic processes cannot be completely excluded, and this issue warrants further investigation. Article in Journal/Newspaper Arctic Greenland Utrecht University Repository Arctic Greenland |
institution |
Open Polar |
collection |
Utrecht University Repository |
op_collection_id |
ftunivutrecht |
language |
English |
topic |
DNA methylation Epidemiology Flow cytometry Human spermatozoa Perfluoroalkyl substances Pyrosequencing Coronacrisis-Taverne Health Toxicology and Mutagenesis Genetics(clinical) |
spellingShingle |
DNA methylation Epidemiology Flow cytometry Human spermatozoa Perfluoroalkyl substances Pyrosequencing Coronacrisis-Taverne Health Toxicology and Mutagenesis Genetics(clinical) Leter, Giorgio Consales, Claudia Eleuteri, Patrizia Uccelli, Raffaella Specht, Ina O. Toft, Gunnar Moccia, Tania Budillon, Alfredo Jönsson, Bo A G Lindh, Christian H. Giwercman, Aleksander Pedersen, Henning S. Ludwicki, Jan K. Zviezdai, Valentyna Heederik, Dick Bonde, Jens Peter E Spanò, Marcello Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
topic_facet |
DNA methylation Epidemiology Flow cytometry Human spermatozoa Perfluoroalkyl substances Pyrosequencing Coronacrisis-Taverne Health Toxicology and Mutagenesis Genetics(clinical) |
description |
Perfluoroalkyl substances (PFASs) are widely used in a variety of industrial processes and products, and have been detected globally in humans and wildlife. PFASs are suspected to interfere with endocrine signaling and to adversely affect human reproductive health. The aim of the present study was to investigate the associations between exposure to PFASs and sperm global methylation levels in a population of non-occupationally exposed fertile men. Measurements of PFASs in serum from 262 partners of pregnant women from Greenland, Poland and Ukraine, were also carried out by liquid chromatography tandem mass spectrometry. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexane sulfonic acid (PFHxS), and perfluorononanoic acid (PFNA) were detected in 97% of the blood samples. Two surrogate markers were used to assess DNA global methylation levels in semen samples from the same men: (a) average DNA methylation level in repetitive DNA sequences (Alu, LINE-1, Satα) quantified by PCR-pyrosequencing after bisulfite conversion; (b) flow cytometric immunodetection of 5-methyl-cytosines. After multivariate linear regression analysis, no major consistent associations between PFASs exposure and sperm DNA global methylation endpoints could be detected. However, since weak but statistically significant associations of different PFASs with DNA hypo- and hyper-methylation were found in some of the studied populations, effects of PFASs on sperm epigenetic processes cannot be completely excluded, and this issue warrants further investigation. |
author2 |
LS IRAS EEPI GRA (Gezh.risico-analyse) Risk Assessment of Toxic and Immunomodulatory Agents IRAS RATIA-SIB |
format |
Article in Journal/Newspaper |
author |
Leter, Giorgio Consales, Claudia Eleuteri, Patrizia Uccelli, Raffaella Specht, Ina O. Toft, Gunnar Moccia, Tania Budillon, Alfredo Jönsson, Bo A G Lindh, Christian H. Giwercman, Aleksander Pedersen, Henning S. Ludwicki, Jan K. Zviezdai, Valentyna Heederik, Dick Bonde, Jens Peter E Spanò, Marcello |
author_facet |
Leter, Giorgio Consales, Claudia Eleuteri, Patrizia Uccelli, Raffaella Specht, Ina O. Toft, Gunnar Moccia, Tania Budillon, Alfredo Jönsson, Bo A G Lindh, Christian H. Giwercman, Aleksander Pedersen, Henning S. Ludwicki, Jan K. Zviezdai, Valentyna Heederik, Dick Bonde, Jens Peter E Spanò, Marcello |
author_sort |
Leter, Giorgio |
title |
Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
title_short |
Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
title_full |
Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
title_fullStr |
Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
title_full_unstemmed |
Exposure to perfluoroalkyl substances and sperm DNA global methylation in arctic and European populations |
title_sort |
exposure to perfluoroalkyl substances and sperm dna global methylation in arctic and european populations |
publishDate |
2014 |
url |
https://dspace.library.uu.nl/handle/1874/305607 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland |
genre_facet |
Arctic Greenland |
op_relation |
0893-6692 https://dspace.library.uu.nl/handle/1874/305607 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1772180058678493184 |