A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance
RATIONALE: Dissolved organic carbon (DOC) plays an important role in carbon cycling, making precise and routine measurement of delta C-13 values and DOC concentration highly desirable. A new promising system has been developed for this purpose. However, broad-scale application of this new technique...
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ftunivutrecht:oai:dspace.library.uu.nl:1874/303479 2023-11-12T04:13:56+01:00 A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance Kirkels, F. M. S. A. Cerli, C. Federherr, E. Gao, J. Kalbitz, K. non-UU output of UU-AW members 2014-12-15 application/pdf https://dspace.library.uu.nl/handle/1874/303479 en eng 0951-4198 https://dspace.library.uu.nl/handle/1874/303479 info:eu-repo/semantics/OpenAccess RATIO MASS-SPECTROMETRY WET CHEMICAL OXIDATION HIGH ARCTIC GLACIER INORGANIC CARBON TERRESTRIAL ECOSYSTEMS C-13 ANALYSIS MATTER SOIL WATERS ANALYZER Article 2014 ftunivutrecht 2023-11-01T23:12:33Z RATIONALE: Dissolved organic carbon (DOC) plays an important role in carbon cycling, making precise and routine measurement of delta C-13 values and DOC concentration highly desirable. A new promising system has been developed for this purpose. However, broad-scale application of this new technique requires an in-depth assessment of analytical performance, and this is described here. METHODS: A high-temperature combustion Total Organic Carbon analyzer was interfaced with continuous flow isotope ratio mass spectrometry (TOC/IRMS) for the simultaneous analysis of the bulk DOC concentration and delta C-13 signature. The analytical performance (precision, memory effects, linearity, volume/concentration effects, accuracy) was thoroughly evaluated, including realistic and challenging conditions such as low DOC concentrations and natural DOC. RESULTS: High precision (standard deviation, SD predominantly ≤0.15 ‰) and accuracy (R2 = 0.9997) were achieved for the δ13C analysis of a broad diversity of DOC solutions. Simultaneously, good results were obtained for the measurement of DOC concentration. Assessment of natural abundance and slightly 13C-enriched DOC, a wide range of concentrations (~0.2–150 mgC/L) and injection volumes (0.05–3 mL), demonstrated minor/negligible memory effects, good linearity and flexible usage. Finally, TOC/IRMS was successfully applied to determine low DOC concentrations (<2 mgC/L) and DOC from diverse terrestrial, freshwater and marine environments (SD ≤0.23 ‰). CONCLUSIONS: TOC/IRMS enables fast and reliable measurement of DOC concentrations and delta C-13 values in aqueous samples, without pre-concentration and freeze-drying. Further investigations should focus on complex, saline matrices and very low DOC concentrations, to achieve a potential lower limit of 0.2 mgC/L. Thus, TOC/IRMS will give DOC research in terrestrial and aquatic environments a huge impulse with high-resolution, routine delta C-13 analysis. Copyright (C) 2014 John Wiley & Sons, Ltd. Article in Journal/Newspaper Arctic Utrecht University Repository Arctic |
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Utrecht University Repository |
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ftunivutrecht |
language |
English |
topic |
RATIO MASS-SPECTROMETRY WET CHEMICAL OXIDATION HIGH ARCTIC GLACIER INORGANIC CARBON TERRESTRIAL ECOSYSTEMS C-13 ANALYSIS MATTER SOIL WATERS ANALYZER |
spellingShingle |
RATIO MASS-SPECTROMETRY WET CHEMICAL OXIDATION HIGH ARCTIC GLACIER INORGANIC CARBON TERRESTRIAL ECOSYSTEMS C-13 ANALYSIS MATTER SOIL WATERS ANALYZER Kirkels, F. M. S. A. Cerli, C. Federherr, E. Gao, J. Kalbitz, K. A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
topic_facet |
RATIO MASS-SPECTROMETRY WET CHEMICAL OXIDATION HIGH ARCTIC GLACIER INORGANIC CARBON TERRESTRIAL ECOSYSTEMS C-13 ANALYSIS MATTER SOIL WATERS ANALYZER |
description |
RATIONALE: Dissolved organic carbon (DOC) plays an important role in carbon cycling, making precise and routine measurement of delta C-13 values and DOC concentration highly desirable. A new promising system has been developed for this purpose. However, broad-scale application of this new technique requires an in-depth assessment of analytical performance, and this is described here. METHODS: A high-temperature combustion Total Organic Carbon analyzer was interfaced with continuous flow isotope ratio mass spectrometry (TOC/IRMS) for the simultaneous analysis of the bulk DOC concentration and delta C-13 signature. The analytical performance (precision, memory effects, linearity, volume/concentration effects, accuracy) was thoroughly evaluated, including realistic and challenging conditions such as low DOC concentrations and natural DOC. RESULTS: High precision (standard deviation, SD predominantly ≤0.15 ‰) and accuracy (R2 = 0.9997) were achieved for the δ13C analysis of a broad diversity of DOC solutions. Simultaneously, good results were obtained for the measurement of DOC concentration. Assessment of natural abundance and slightly 13C-enriched DOC, a wide range of concentrations (~0.2–150 mgC/L) and injection volumes (0.05–3 mL), demonstrated minor/negligible memory effects, good linearity and flexible usage. Finally, TOC/IRMS was successfully applied to determine low DOC concentrations (<2 mgC/L) and DOC from diverse terrestrial, freshwater and marine environments (SD ≤0.23 ‰). CONCLUSIONS: TOC/IRMS enables fast and reliable measurement of DOC concentrations and delta C-13 values in aqueous samples, without pre-concentration and freeze-drying. Further investigations should focus on complex, saline matrices and very low DOC concentrations, to achieve a potential lower limit of 0.2 mgC/L. Thus, TOC/IRMS will give DOC research in terrestrial and aquatic environments a huge impulse with high-resolution, routine delta C-13 analysis. Copyright (C) 2014 John Wiley & Sons, Ltd. |
author2 |
non-UU output of UU-AW members |
format |
Article in Journal/Newspaper |
author |
Kirkels, F. M. S. A. Cerli, C. Federherr, E. Gao, J. Kalbitz, K. |
author_facet |
Kirkels, F. M. S. A. Cerli, C. Federherr, E. Gao, J. Kalbitz, K. |
author_sort |
Kirkels, F. M. S. A. |
title |
A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
title_short |
A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
title_full |
A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
title_fullStr |
A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
title_full_unstemmed |
A novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: II optimization and assessment of analytical performance |
title_sort |
novel high-temperature combustion based system for stable isotope analysis of dissolved organic carbon in aqueous samples.: ii optimization and assessment of analytical performance |
publishDate |
2014 |
url |
https://dspace.library.uu.nl/handle/1874/303479 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
0951-4198 https://dspace.library.uu.nl/handle/1874/303479 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1782331701008007168 |