Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses
Various genetic aspects of the toxic diatom Pseudo-nitzschia species in the northwestern Adriatic Sea were investigated. For this area, limited or no knowledge is available regarding the genetic diversity and geographical patterns of Pseudonitzschia spp., as well as the toxin content. Phylogenetic a...
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Online Access: | http://hdl.handle.net/11576/2538574 https://doi.org/10.1093/plankt/fbs093 |
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ftunivurbino:oai:ora.uniurb.it:11576/2538574 2024-04-14T08:15:55+00:00 Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses PENNA, ANTONELLA CASABIANCA, SILVIA PERINI, FEDERICO Bastianini M. Riccardi E. Pigozzi S. Scardi M. Penna, Antonella Casabianca, Silvia Perini, Federico Bastianini, M. Riccardi, E. Pigozzi, S. Scardi, M. 2013 ELETTRONICO http://hdl.handle.net/11576/2538574 https://doi.org/10.1093/plankt/fbs093 eng eng info:eu-repo/semantics/altIdentifier/wos/000316129700010 issue:35 firstpage:352 lastpage:366 numberofpages:15 journal:JOURNAL OF PLANKTON RESEARCH http://hdl.handle.net/11576/2538574 doi:10.1093/plankt/fbs093 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84874845644 domoic acid genetic identification HAB NWAdriatic Sea Pseudonitzschia spp. qrt-PCR info:eu-repo/semantics/article 2013 ftunivurbino https://doi.org/10.1093/plankt/fbs093 2024-03-21T17:01:51Z Various genetic aspects of the toxic diatom Pseudo-nitzschia species in the northwestern Adriatic Sea were investigated. For this area, limited or no knowledge is available regarding the genetic diversity and geographical patterns of Pseudonitzschia spp., as well as the toxin content. Phylogenetic analyses identified strains belonging to P. delicatissima, P. calliantha, P. pungens and P. mannii. Networks of haplotypes inferred from Adriatic and worldwide strains revealed two main haplotypes in P. delicatissima from the Mediterranean and north Atlantic, with a single panmictic population in P. calliantha, and Adriatic P. pungens strains sharing the most frequent haplotype. The quantitative real-time PCR (qrt-PCR) assay was developed to estimate the number of rDNA copies and their variation among Pseudo-nitzschia species and strains. Qrt-PCR analysis showed that P. delicatissima and P. calliantha had different average rDNA copy numbers per cell (P, 0.001). It is suggested that different rDNA copy numbers among species might be used to discriminate between morphotypes identified using light microscopy. We also discuss how the rDNA copy number variability found among P. pungens strains from different months (P, 0.001) may relate to physiological activities and/or adaptative strategies. Northwestern Adriatic P. delicatissima strains produced domoic acid at low concentrations. Article in Journal/Newspaper North Atlantic Università degli Studi di Urbino: CINECA IRIS Journal of Plankton Research 35 2 352 366 |
institution |
Open Polar |
collection |
Università degli Studi di Urbino: CINECA IRIS |
op_collection_id |
ftunivurbino |
language |
English |
topic |
domoic acid genetic identification HAB NWAdriatic Sea Pseudonitzschia spp. qrt-PCR |
spellingShingle |
domoic acid genetic identification HAB NWAdriatic Sea Pseudonitzschia spp. qrt-PCR PENNA, ANTONELLA CASABIANCA, SILVIA PERINI, FEDERICO Bastianini M. Riccardi E. Pigozzi S. Scardi M. Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
topic_facet |
domoic acid genetic identification HAB NWAdriatic Sea Pseudonitzschia spp. qrt-PCR |
description |
Various genetic aspects of the toxic diatom Pseudo-nitzschia species in the northwestern Adriatic Sea were investigated. For this area, limited or no knowledge is available regarding the genetic diversity and geographical patterns of Pseudonitzschia spp., as well as the toxin content. Phylogenetic analyses identified strains belonging to P. delicatissima, P. calliantha, P. pungens and P. mannii. Networks of haplotypes inferred from Adriatic and worldwide strains revealed two main haplotypes in P. delicatissima from the Mediterranean and north Atlantic, with a single panmictic population in P. calliantha, and Adriatic P. pungens strains sharing the most frequent haplotype. The quantitative real-time PCR (qrt-PCR) assay was developed to estimate the number of rDNA copies and their variation among Pseudo-nitzschia species and strains. Qrt-PCR analysis showed that P. delicatissima and P. calliantha had different average rDNA copy numbers per cell (P, 0.001). It is suggested that different rDNA copy numbers among species might be used to discriminate between morphotypes identified using light microscopy. We also discuss how the rDNA copy number variability found among P. pungens strains from different months (P, 0.001) may relate to physiological activities and/or adaptative strategies. Northwestern Adriatic P. delicatissima strains produced domoic acid at low concentrations. |
author2 |
Penna, Antonella Casabianca, Silvia Perini, Federico Bastianini, M. Riccardi, E. Pigozzi, S. Scardi, M. |
format |
Article in Journal/Newspaper |
author |
PENNA, ANTONELLA CASABIANCA, SILVIA PERINI, FEDERICO Bastianini M. Riccardi E. Pigozzi S. Scardi M. |
author_facet |
PENNA, ANTONELLA CASABIANCA, SILVIA PERINI, FEDERICO Bastianini M. Riccardi E. Pigozzi S. Scardi M. |
author_sort |
PENNA, ANTONELLA |
title |
Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
title_short |
Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
title_full |
Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
title_fullStr |
Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
title_full_unstemmed |
Toxic Pseudo-nitzschia spp. in the northwestern Adriatic Sea: characterization of species composition by genetic and molecular quantitative analyses |
title_sort |
toxic pseudo-nitzschia spp. in the northwestern adriatic sea: characterization of species composition by genetic and molecular quantitative analyses |
publishDate |
2013 |
url |
http://hdl.handle.net/11576/2538574 https://doi.org/10.1093/plankt/fbs093 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000316129700010 issue:35 firstpage:352 lastpage:366 numberofpages:15 journal:JOURNAL OF PLANKTON RESEARCH http://hdl.handle.net/11576/2538574 doi:10.1093/plankt/fbs093 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84874845644 |
op_doi |
https://doi.org/10.1093/plankt/fbs093 |
container_title |
Journal of Plankton Research |
container_volume |
35 |
container_issue |
2 |
container_start_page |
352 |
op_container_end_page |
366 |
_version_ |
1796314419448250368 |