Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet
Siberian sturgeon (Acipenser baerii) represents a high-value fish farmed for obtaining high-priced food. In this study, the occurrence of antibiotic resistance (AR) genes encoding for resistance to antimicrobial administered in clinical practice, as aminoglycosides, -lactams, macrolide-lincosamide-s...
Published in: | Aquaculture |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/11390/1227977 https://doi.org/10.1016/j.aquaculture.2022.738485 |
id |
ftunivudineiris:oai:air.uniud.it:11390/1227977 |
---|---|
record_format |
openpolar |
spelling |
ftunivudineiris:oai:air.uniud.it:11390/1227977 2023-07-30T03:55:24+02:00 Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea 2022 ELETTRONICO http://hdl.handle.net/11390/1227977 https://doi.org/10.1016/j.aquaculture.2022.738485 eng eng volume:560 firstpage:738485 journal:AQUACULTURE http://hdl.handle.net/11390/1227977 doi:10.1016/j.aquaculture.2022.738485 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85132766910 edible insect tetracycline erythromycin qPCR risk assessment info:eu-repo/semantics/article 2022 ftunivudineiris https://doi.org/10.1016/j.aquaculture.2022.738485 2023-07-18T20:23:23Z Siberian sturgeon (Acipenser baerii) represents a high-value fish farmed for obtaining high-priced food. In this study, the occurrence of antibiotic resistance (AR) genes encoding for resistance to antimicrobial administered in clinical practice, as aminoglycosides, -lactams, macrolide-lincosamide-streptogramin B, tetracyclines, and vancomycin, were studied in the gut of sturgeons fed a diet containing 50% Hermetia illucens larvae by qPCR assays. Erm(A), erm(B), erm(C), tet(K), tet(M), tet(S), and tet(W) were detected in all samples. Feed and guts of sturgeons at beginning of rearing were negative for tet(O), vanA, vanB, mecA, and aac-aph. BlaZ was exclusively detected in control diet. The highest copy numbers were observed for tet(M), tet(K) and tet(S) in control diet. The copy numbers of most of the genes were higher in control diet than in diet containing 50% insect. Despite the differences in the gene copy numbers in control or insect-based diet, the results for AR genes contained in the guts of fish highlighted that, at medium levels of substitution (50%), no effect of the insect-based diet on the AR genes seems to occur in the experimental sturgeons at the end of rearing. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Università degli Studi di Udine: CINECA IRIS Aquaculture 560 738485 |
institution |
Open Polar |
collection |
Università degli Studi di Udine: CINECA IRIS |
op_collection_id |
ftunivudineiris |
language |
English |
topic |
edible insect tetracycline erythromycin qPCR risk assessment |
spellingShingle |
edible insect tetracycline erythromycin qPCR risk assessment Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
topic_facet |
edible insect tetracycline erythromycin qPCR risk assessment |
description |
Siberian sturgeon (Acipenser baerii) represents a high-value fish farmed for obtaining high-priced food. In this study, the occurrence of antibiotic resistance (AR) genes encoding for resistance to antimicrobial administered in clinical practice, as aminoglycosides, -lactams, macrolide-lincosamide-streptogramin B, tetracyclines, and vancomycin, were studied in the gut of sturgeons fed a diet containing 50% Hermetia illucens larvae by qPCR assays. Erm(A), erm(B), erm(C), tet(K), tet(M), tet(S), and tet(W) were detected in all samples. Feed and guts of sturgeons at beginning of rearing were negative for tet(O), vanA, vanB, mecA, and aac-aph. BlaZ was exclusively detected in control diet. The highest copy numbers were observed for tet(M), tet(K) and tet(S) in control diet. The copy numbers of most of the genes were higher in control diet than in diet containing 50% insect. Despite the differences in the gene copy numbers in control or insect-based diet, the results for AR genes contained in the guts of fish highlighted that, at medium levels of substitution (50%), no effect of the insect-based diet on the AR genes seems to occur in the experimental sturgeons at the end of rearing. |
author2 |
Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea |
format |
Article in Journal/Newspaper |
author |
Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea |
author_facet |
Milanović, Vesna Cardinali, Federica Aquilanti, Lucia Maoloni, Antonietta Garofalo, Cristiana Zarantoniello, Matteo Olivotto, Ike Riolo, Paola Ruschioni, Sara Isidoro, Nunzio Corsi, Lorenzo Cardinaletti, Gloriana Osimani, Andrea |
author_sort |
Milanović, Vesna |
title |
Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
title_short |
Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
title_full |
Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
title_fullStr |
Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
title_full_unstemmed |
Quantification of antibiotic resistance genes in Siberian sturgeons (Acipenser baerii) fed Hermetia illucens-based diet |
title_sort |
quantification of antibiotic resistance genes in siberian sturgeons (acipenser baerii) fed hermetia illucens-based diet |
publishDate |
2022 |
url |
http://hdl.handle.net/11390/1227977 https://doi.org/10.1016/j.aquaculture.2022.738485 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_relation |
volume:560 firstpage:738485 journal:AQUACULTURE http://hdl.handle.net/11390/1227977 doi:10.1016/j.aquaculture.2022.738485 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85132766910 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2022.738485 |
container_title |
Aquaculture |
container_volume |
560 |
container_start_page |
738485 |
_version_ |
1772810355524763648 |