A hydrogel-based enzyme-loaded polymersome reactor
In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs un...
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ftunivtwente:oai:doc.utwente.nl:77542 2023-05-15T13:38:56+02:00 A hydrogel-based enzyme-loaded polymersome reactor Hoog, Hans-Peter de Arends, Isabel W.C.E. Rowan, Alan E. Cornelissen, Jeroen J.L.M. Nolte, Roeland J.M. 2010 application/pdf http://purl.utwente.nl/publications/77542 unknown Royal Society of Chemistry http://doc.utwente.nl/77542/1/hydrogel.pdf http://purl.utwente.nl/publications/77542 © 2010 Royal Society of Chemistry Article / Letter to editor 2010 ftunivtwente 2016-10-26T22:18:32Z In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx). Article in Journal/Newspaper Antarc* University of Twente Publications |
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University of Twente Publications |
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ftunivtwente |
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description |
In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx). |
format |
Article in Journal/Newspaper |
author |
Hoog, Hans-Peter de Arends, Isabel W.C.E. Rowan, Alan E. Cornelissen, Jeroen J.L.M. Nolte, Roeland J.M. |
spellingShingle |
Hoog, Hans-Peter de Arends, Isabel W.C.E. Rowan, Alan E. Cornelissen, Jeroen J.L.M. Nolte, Roeland J.M. A hydrogel-based enzyme-loaded polymersome reactor |
author_facet |
Hoog, Hans-Peter de Arends, Isabel W.C.E. Rowan, Alan E. Cornelissen, Jeroen J.L.M. Nolte, Roeland J.M. |
author_sort |
Hoog, Hans-Peter de |
title |
A hydrogel-based enzyme-loaded polymersome reactor |
title_short |
A hydrogel-based enzyme-loaded polymersome reactor |
title_full |
A hydrogel-based enzyme-loaded polymersome reactor |
title_fullStr |
A hydrogel-based enzyme-loaded polymersome reactor |
title_full_unstemmed |
A hydrogel-based enzyme-loaded polymersome reactor |
title_sort |
hydrogel-based enzyme-loaded polymersome reactor |
publisher |
Royal Society of Chemistry |
publishDate |
2010 |
url |
http://purl.utwente.nl/publications/77542 |
genre |
Antarc* |
genre_facet |
Antarc* |
op_relation |
http://doc.utwente.nl/77542/1/hydrogel.pdf http://purl.utwente.nl/publications/77542 |
op_rights |
© 2010 Royal Society of Chemistry |
_version_ |
1766112616527167488 |