Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions
The Moon is characterized by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere and high ionizing radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For l...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/2067/47725 https://doi.org/10.1111/1462-2920.15995 https://api.elsevier.com/content/abstract/scopus_id/85128706153 |
id |
ftunivtuscia:oai:dspace.unitus.it:2067/47725 |
---|---|
record_format |
openpolar |
spelling |
ftunivtuscia:oai:dspace.unitus.it:2067/47725 2023-05-15T13:43:45+02:00 Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions Cassaro, Alessia Pacelli, Claudia Baqué, Mickael Cavalazzi, Barbara Gasparotto, Giorgio Saladino, Raffaele Lorenzo Botta Böttger, Ute Rabbow, Elke de Vera, Jean-Pierre Onofri, Silvano ITA 2022 ELETTRONICO http://hdl.handle.net/2067/47725 https://doi.org/10.1111/1462-2920.15995 https://api.elsevier.com/content/abstract/scopus_id/85128706153 en eng ENVIRONMENTAL MICROBIOLOGY 1462-2920 http://hdl.handle.net/2067/47725 doi:10.1111/1462-2920.15995 35437941 2-s2.0-85128706153 https://api.elsevier.com/content/abstract/scopus_id/85128706153 restricted article 2022 ftunivtuscia https://doi.org/10.1111/1462-2920.15995 2022-05-17T22:11:40Z The Moon is characterized by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere and high ionizing radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For lunar exploration with the Lunar Gateway platform, exposure experiments in Low Earth Orbit are useful testbeds to prepare for lunar space experiments and to understand how and if potential biomarkers are influenced by extra-terrestrial conditions. During the BIOMEX (BIOlogy and Mars EXperiment) project, dried colonies of the fungus Cryomyces antarcticus grown on Lunar Regolith Analogue (LRA) were exposed to space conditions for 16 months aboard the EXPOSE-R2 payload outside the International Space Station. In this study, we investigated the stability/degradation of fungal biomarkers in LRA after exposure to (i) simulated space and (ii) real space conditions, using Raman spectroscopy, gas chromatography-mass spectrometry and DNA amplification. The results demonstrated that fungal biomarkers were detectable after 16 months of real space exposure. This work will contribute to the interpretation of data from future biological experiments in the Cislunar orbit with the Lunar Gateway platform and/or on the lunar surface, in preparation for the next step of human exploration. 11 sì Article in Journal/Newspaper Antarc* antarcticus Università degli studi della Tuscia: Unitus DSpace Environmental Microbiology |
institution |
Open Polar |
collection |
Università degli studi della Tuscia: Unitus DSpace |
op_collection_id |
ftunivtuscia |
language |
English |
description |
The Moon is characterized by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere and high ionizing radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For lunar exploration with the Lunar Gateway platform, exposure experiments in Low Earth Orbit are useful testbeds to prepare for lunar space experiments and to understand how and if potential biomarkers are influenced by extra-terrestrial conditions. During the BIOMEX (BIOlogy and Mars EXperiment) project, dried colonies of the fungus Cryomyces antarcticus grown on Lunar Regolith Analogue (LRA) were exposed to space conditions for 16 months aboard the EXPOSE-R2 payload outside the International Space Station. In this study, we investigated the stability/degradation of fungal biomarkers in LRA after exposure to (i) simulated space and (ii) real space conditions, using Raman spectroscopy, gas chromatography-mass spectrometry and DNA amplification. The results demonstrated that fungal biomarkers were detectable after 16 months of real space exposure. This work will contribute to the interpretation of data from future biological experiments in the Cislunar orbit with the Lunar Gateway platform and/or on the lunar surface, in preparation for the next step of human exploration. 11 sì |
author2 |
ITA |
format |
Article in Journal/Newspaper |
author |
Cassaro, Alessia Pacelli, Claudia Baqué, Mickael Cavalazzi, Barbara Gasparotto, Giorgio Saladino, Raffaele Lorenzo Botta Böttger, Ute Rabbow, Elke de Vera, Jean-Pierre Onofri, Silvano |
spellingShingle |
Cassaro, Alessia Pacelli, Claudia Baqué, Mickael Cavalazzi, Barbara Gasparotto, Giorgio Saladino, Raffaele Lorenzo Botta Böttger, Ute Rabbow, Elke de Vera, Jean-Pierre Onofri, Silvano Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
author_facet |
Cassaro, Alessia Pacelli, Claudia Baqué, Mickael Cavalazzi, Barbara Gasparotto, Giorgio Saladino, Raffaele Lorenzo Botta Böttger, Ute Rabbow, Elke de Vera, Jean-Pierre Onofri, Silvano |
author_sort |
Cassaro, Alessia |
title |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_short |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_full |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_fullStr |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_full_unstemmed |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_sort |
investigation of fungal biomolecules after low earth orbit exposure: a testbed for the next moon missions |
publishDate |
2022 |
url |
http://hdl.handle.net/2067/47725 https://doi.org/10.1111/1462-2920.15995 https://api.elsevier.com/content/abstract/scopus_id/85128706153 |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_relation |
ENVIRONMENTAL MICROBIOLOGY 1462-2920 http://hdl.handle.net/2067/47725 doi:10.1111/1462-2920.15995 35437941 2-s2.0-85128706153 https://api.elsevier.com/content/abstract/scopus_id/85128706153 |
op_rights |
restricted |
op_doi |
https://doi.org/10.1111/1462-2920.15995 |
container_title |
Environmental Microbiology |
_version_ |
1766192684212420608 |