Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity
Antimicrobial peptides (AMPs) are short peptides active against a wide range of pathogens and, therefore, they are considered a useful alternative to conventional antibiotics. We have identified a new AMP in a transcriptome derived from the Antarctic fish Trematomus bernacchii. This peptide, named T...
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ftunivtuscia:oai:dspace.unitus.it:2067/42578 2023-05-15T13:52:30+02:00 Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity Della Pelle, Giulia Perà, Giulia Belardinelli, Maria Cristina Gerdol, Marco Felli, Martina Crognale, Silvia Scapigliati, Giuseppe Ceccacci, Francesca Buonocore, Francesco Porcelli, Fernando ITA 2020 http://hdl.handle.net/2067/42578 https://doi.org/10.3390/antibiotics9020066 https://www.mdpi.com/2079-6382/9/2/66 en eng ANTIBIOTICS 66 9 2 2079-6382 http://hdl.handle.net/2067/42578 doi:10.3390/antibiotics9020066 32041161 2-s2.0-85079871941 https://www.mdpi.com/2079-6382/9/2/66 restricted article 2020 ftunivtuscia https://doi.org/10.3390/antibiotics9020066 2022-05-01T15:19:44Z Antimicrobial peptides (AMPs) are short peptides active against a wide range of pathogens and, therefore, they are considered a useful alternative to conventional antibiotics. We have identified a new AMP in a transcriptome derived from the Antarctic fish Trematomus bernacchii. This peptide, named Trematocine, has been investigated for its expression both at the basal level and after in vivo immunization with an endemic Antarctic bacterium (Psychrobacter sp. TAD1). Results agree with the expected behavior of a fish innate immune component, therefore we decided to synthesize the putative mature sequence of Trematocine to determine the structure, the interaction with biological membranes, and the biological activity. We showed that Trematocine folds into a α-helical structure in the presence of both zwitterionic and anionic charged vesicles. We demonstrated that Trematocine has a highly specific interaction with anionic charged vesicles and that it can kill Gram-negative bacteria, possibly via a carpet like mechanism. Moreover, Trematocine showed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against selected Gram-positive and Gram-negative bacteria similar to other AMPs isolated from Antarctic fishes. The peptide is a possible candidate for a new drug as it does not show any haemolytic or cytotoxic activity against mammalian cells at the concentration needed to kill the tested bacteria. Article in Journal/Newspaper Antarc* Antarctic Università degli studi della Tuscia: Unitus DSpace Antarctic The Antarctic Antibiotics 9 2 66 |
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Università degli studi della Tuscia: Unitus DSpace |
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ftunivtuscia |
language |
English |
description |
Antimicrobial peptides (AMPs) are short peptides active against a wide range of pathogens and, therefore, they are considered a useful alternative to conventional antibiotics. We have identified a new AMP in a transcriptome derived from the Antarctic fish Trematomus bernacchii. This peptide, named Trematocine, has been investigated for its expression both at the basal level and after in vivo immunization with an endemic Antarctic bacterium (Psychrobacter sp. TAD1). Results agree with the expected behavior of a fish innate immune component, therefore we decided to synthesize the putative mature sequence of Trematocine to determine the structure, the interaction with biological membranes, and the biological activity. We showed that Trematocine folds into a α-helical structure in the presence of both zwitterionic and anionic charged vesicles. We demonstrated that Trematocine has a highly specific interaction with anionic charged vesicles and that it can kill Gram-negative bacteria, possibly via a carpet like mechanism. Moreover, Trematocine showed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against selected Gram-positive and Gram-negative bacteria similar to other AMPs isolated from Antarctic fishes. The peptide is a possible candidate for a new drug as it does not show any haemolytic or cytotoxic activity against mammalian cells at the concentration needed to kill the tested bacteria. |
author2 |
ITA |
format |
Article in Journal/Newspaper |
author |
Della Pelle, Giulia Perà, Giulia Belardinelli, Maria Cristina Gerdol, Marco Felli, Martina Crognale, Silvia Scapigliati, Giuseppe Ceccacci, Francesca Buonocore, Francesco Porcelli, Fernando |
spellingShingle |
Della Pelle, Giulia Perà, Giulia Belardinelli, Maria Cristina Gerdol, Marco Felli, Martina Crognale, Silvia Scapigliati, Giuseppe Ceccacci, Francesca Buonocore, Francesco Porcelli, Fernando Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
author_facet |
Della Pelle, Giulia Perà, Giulia Belardinelli, Maria Cristina Gerdol, Marco Felli, Martina Crognale, Silvia Scapigliati, Giuseppe Ceccacci, Francesca Buonocore, Francesco Porcelli, Fernando |
author_sort |
Della Pelle, Giulia |
title |
Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
title_short |
Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
title_full |
Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
title_fullStr |
Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
title_full_unstemmed |
Trematocine, a Novel Antimicrobial Peptide from the Antarctic Fish Trematomus bernacchii: Identification and Biological Activity |
title_sort |
trematocine, a novel antimicrobial peptide from the antarctic fish trematomus bernacchii: identification and biological activity |
publishDate |
2020 |
url |
http://hdl.handle.net/2067/42578 https://doi.org/10.3390/antibiotics9020066 https://www.mdpi.com/2079-6382/9/2/66 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
ANTIBIOTICS 66 9 2 2079-6382 http://hdl.handle.net/2067/42578 doi:10.3390/antibiotics9020066 32041161 2-s2.0-85079871941 https://www.mdpi.com/2079-6382/9/2/66 |
op_rights |
restricted |
op_doi |
https://doi.org/10.3390/antibiotics9020066 |
container_title |
Antibiotics |
container_volume |
9 |
container_issue |
2 |
container_start_page |
66 |
_version_ |
1766256812558909440 |