Sanguiin H-6 Fractionated from Cloudberry (Rubus chamaemorus) Seeds Can Prevent the Methicillin-Resistant Staphylococcus aureus Biofilm Development during Wound Infection

• Published in: Antibiotics
• Main Authors: Aguilera-Correa John Jairo, Esteban Jaime, Pérez-Rial Sandra, Cuadros Juan, Oksman-Caldentey Kirsi-Marja, Kinnari Teemu J., Alakomi Hanna-Leena, Perez-Tanoira Ramon, Cuñas-Figueroa Iskra Denise, Nohynek Liisa, Puupponen-Pimiä Riitta, Fernández-López Sara, Salminen Juha-Pekka
• Other Authors: orgaaninen kemia ja kemiallinen biologia, Laboratory of Organic Chemistry and Chemical Biology, 2606303
• Language: English
• Published: MDPI 2022
• Subjects: Rubus chamaemorus
• Online Access: https://www.utupub.fi/handle/10024/163701; https://doi.org/10.3390/antibiotics10121481

Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.