Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction
We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The e...
Main Authors: | , , , , , , , |
---|---|
Other Authors: | , , , |
Language: | English |
Published: |
American Chemical Society
2022
|
Subjects: | |
Online Access: | https://www.utupub.fi/handle/10024/160326 https://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00109 |
id |
ftunivturku:oai:www.utupub.fi:10024/160326 |
---|---|
record_format |
openpolar |
spelling |
ftunivturku:oai:www.utupub.fi:10024/160326 2023-05-15T18:07:46+02:00 Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction Honkapää Kaisu Suvanto Jussi Seppänen-Laakso Tuulikki Salminen Juha-Pekka Oksman-Caldentey Kirsi-Marja Puupponen-Pimiä Riitta Nohynek Liisa Tähtiharju Juha kemian laitoksen yhteiset, Department of Chemistry orgaaninen kemia ja kemiallinen biologia, Laboratory of Organic Chemistry and Chemical Biology 2606300 2606303 2022-10-28T12:33:01Z 917 927 https://www.utupub.fi/handle/10024/160326 https://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00109 en eng American Chemical Society United States Yhdysvallat (USA) US 1 10.1021/acsfoodscitech.0c00109 ACS Food Science & Technology 5 https://www.utupub.fi/handle/10024/160326 https://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00109 URN:NBN:fi-fe2022020818044 2692-1944 2022 ftunivturku 2022-11-03T00:00:02Z We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus . This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing. Other/Unknown Material Rubus chamaemorus University of Turku: UTUPub |
institution |
Open Polar |
collection |
University of Turku: UTUPub |
op_collection_id |
ftunivturku |
language |
English |
description |
We have developed an organic solvent-free process to enrich natural antimicrobials from the important Nordic Rubus berry species, especially from cloudberry. The process utilizes industrial berry byproducts as raw-material, and it is based on seed sanding technology and water-based extraction. The extracts showed strong antimicrobial activity against Gram-positive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). A concentration of 5 mg/mL totally eliminated these bacteria in 24 h. Effects against Gram-negative bacteria were modest. Polyphenol analysis of the hydrothermal extracts showed that the antimicrobial activity is correlated to ellagitannins, mainly dimeric sanguiin H-6 and sanguiin H-10 isomers. Sanguiin H-10 is not commonly found in intact cloudberry seeds, but it is formed from the dimeric and trimeric ellagitannins, during the extraction. Surprisingly hydrothermal extracts had no or minor effects on beneficial bacteria, Lactobacillus rhamnosus . This interesting finding might offer an application potential in controlling skin microbial pathogens, for example, in wound healing. |
author2 |
kemian laitoksen yhteiset, Department of Chemistry orgaaninen kemia ja kemiallinen biologia, Laboratory of Organic Chemistry and Chemical Biology 2606300 2606303 |
author |
Honkapää Kaisu Suvanto Jussi Seppänen-Laakso Tuulikki Salminen Juha-Pekka Oksman-Caldentey Kirsi-Marja Puupponen-Pimiä Riitta Nohynek Liisa Tähtiharju Juha |
spellingShingle |
Honkapää Kaisu Suvanto Jussi Seppänen-Laakso Tuulikki Salminen Juha-Pekka Oksman-Caldentey Kirsi-Marja Puupponen-Pimiä Riitta Nohynek Liisa Tähtiharju Juha Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
author_facet |
Honkapää Kaisu Suvanto Jussi Seppänen-Laakso Tuulikki Salminen Juha-Pekka Oksman-Caldentey Kirsi-Marja Puupponen-Pimiä Riitta Nohynek Liisa Tähtiharju Juha |
author_sort |
Honkapää Kaisu |
title |
Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
title_short |
Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
title_full |
Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
title_fullStr |
Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
title_full_unstemmed |
Natural Antimicrobials from Cloudberry (Rubus chamaemorus) Seeds by Sanding and Hydrothermal Extraction |
title_sort |
natural antimicrobials from cloudberry (rubus chamaemorus) seeds by sanding and hydrothermal extraction |
publisher |
American Chemical Society |
publishDate |
2022 |
url |
https://www.utupub.fi/handle/10024/160326 https://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00109 |
genre |
Rubus chamaemorus |
genre_facet |
Rubus chamaemorus |
op_relation |
1 10.1021/acsfoodscitech.0c00109 ACS Food Science & Technology 5 https://www.utupub.fi/handle/10024/160326 https://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00109 URN:NBN:fi-fe2022020818044 2692-1944 |
_version_ |
1766180007763247104 |