Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging
The enantiomers of aromatic 4-dibenzocyclooctynol (DIBO), used for radiolabeling and subsequent conjugation of biomolecules to form radioligands for positron emission tomography (PET), were separated by kinetic resolution using lipase A from Candida antarctica (CAL-A). In optimized conditions, (R)-D...
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ftunivturku:oai:www.utupub.fi:10024/157374 2023-05-15T13:58:49+02:00 Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging Sirén Saija Salminen Tiina A. Puttreddy Rakesh Liljeblad Arto Rissanen Kari Scheinin Mika Li Xiang-Guo Dahlström Käthe M. PET perustoiminta, PET Basic Operations tyks, vsshp, tyks, vsshp biolääketieteen laitos, yhteiset, Institute of Biomedicine 2607100 2609810 2022-10-27T12:15:39Z https://www.utupub.fi/handle/10024/157374 en eng MDPI AG Switzerland Sveitsi CH 25 10.3390/molecules25040879 Molecules 4 https://www.utupub.fi/handle/10024/157374 URN:NBN:fi-fe2021042822848 1420-3049 2022 ftunivturku 2022-11-02T23:59:19Z The enantiomers of aromatic 4-dibenzocyclooctynol (DIBO), used for radiolabeling and subsequent conjugation of biomolecules to form radioligands for positron emission tomography (PET), were separated by kinetic resolution using lipase A from Candida antarctica (CAL-A). In optimized conditions, (R)-DIBO [(R)-1, ee 95%] and its acetylated (S)-ester [(S)-2, ee 96%] were isolated. In silico docking results explained the ability of CAL-A to differentiate the enantiomers of DIBO and to accommodate various acyl donors. Anhydrous MgCl 2 was used for binding water from the reaction medium and, thus, for obtaining higher conversion by preventing hydrolysis of the product (S)-2 into the starting material. Since the presence of hydrated MgCl 2 ·6H 2 O also allowed high conversion or effect on enantioselectivity, Mg 2+ ion was suspected to interact with the enzyme. Binding site predictions indicated at least two sites of interest; one in the lid domain at the bottom of the acyl binding pocket and another at the interface of the hydrolase and flap domains, just above the active site. Other/Unknown Material Antarc* Antarctica University of Turku: UTUPub |
institution |
Open Polar |
collection |
University of Turku: UTUPub |
op_collection_id |
ftunivturku |
language |
English |
description |
The enantiomers of aromatic 4-dibenzocyclooctynol (DIBO), used for radiolabeling and subsequent conjugation of biomolecules to form radioligands for positron emission tomography (PET), were separated by kinetic resolution using lipase A from Candida antarctica (CAL-A). In optimized conditions, (R)-DIBO [(R)-1, ee 95%] and its acetylated (S)-ester [(S)-2, ee 96%] were isolated. In silico docking results explained the ability of CAL-A to differentiate the enantiomers of DIBO and to accommodate various acyl donors. Anhydrous MgCl 2 was used for binding water from the reaction medium and, thus, for obtaining higher conversion by preventing hydrolysis of the product (S)-2 into the starting material. Since the presence of hydrated MgCl 2 ·6H 2 O also allowed high conversion or effect on enantioselectivity, Mg 2+ ion was suspected to interact with the enzyme. Binding site predictions indicated at least two sites of interest; one in the lid domain at the bottom of the acyl binding pocket and another at the interface of the hydrolase and flap domains, just above the active site. |
author2 |
PET perustoiminta, PET Basic Operations tyks, vsshp, tyks, vsshp biolääketieteen laitos, yhteiset, Institute of Biomedicine 2607100 2609810 |
author |
Sirén Saija Salminen Tiina A. Puttreddy Rakesh Liljeblad Arto Rissanen Kari Scheinin Mika Li Xiang-Guo Dahlström Käthe M. |
spellingShingle |
Sirén Saija Salminen Tiina A. Puttreddy Rakesh Liljeblad Arto Rissanen Kari Scheinin Mika Li Xiang-Guo Dahlström Käthe M. Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
author_facet |
Sirén Saija Salminen Tiina A. Puttreddy Rakesh Liljeblad Arto Rissanen Kari Scheinin Mika Li Xiang-Guo Dahlström Käthe M. |
author_sort |
Sirén Saija |
title |
Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
title_short |
Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
title_full |
Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
title_fullStr |
Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
title_full_unstemmed |
Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) Used for PET Imaging |
title_sort |
candida antarctica lipase a-based enantiorecognition of a highly strained 4-dibenzocyclooctynol (dibo) used for pet imaging |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://www.utupub.fi/handle/10024/157374 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
25 10.3390/molecules25040879 Molecules 4 https://www.utupub.fi/handle/10024/157374 URN:NBN:fi-fe2021042822848 1420-3049 |
_version_ |
1766267188790951936 |