Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus

Background Rockweeds are among the most important foundation species of temperate rocky littoral shores. In the Baltic Sea, the rockweed Fucus vesiculosus is distributed along a decreasing salinity gradient from the North Atlantic entrance to the low-salinity regions in the north-eastern margins, th...

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Main Authors: Pereyra RT, Rugiu L, Jormalainen V, Panova M
Other Authors: ekologia ja evoluutiobiologia, Ecology and Evolutionary Biology, 2606402
Language:English
Published: BMC 2022
Subjects:
Online Access:https://www.utupub.fi/handle/10024/156127
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6470-y
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spelling ftunivturku:oai:www.utupub.fi:10024/156127 2023-05-15T17:35:34+02:00 Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus Pereyra RT Rugiu L Jormalainen V Panova M ekologia ja evoluutiobiologia, Ecology and Evolutionary Biology 2606402 2022-10-27T11:57:01Z https://www.utupub.fi/handle/10024/156127 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6470-y en eng BMC United Kingdom Britannia GB 21 ARTN 42 10.1186/s12864-020-6470-y BMC Genomics 1 https://www.utupub.fi/handle/10024/156127 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6470-y URN:NBN:fi-fe2021042822158 1471-2164 2022 ftunivturku 2022-11-02T23:59:02Z Background Rockweeds are among the most important foundation species of temperate rocky littoral shores. In the Baltic Sea, the rockweed Fucus vesiculosus is distributed along a decreasing salinity gradient from the North Atlantic entrance to the low-salinity regions in the north-eastern margins, thus, demonstrating a remarkable tolerance to hyposalinity. The underlying mechanisms for this tolerance are still poorly understood. Here, we exposed F. vesiculosus from two range-margin populations to the hyposaline (2.5 PSU - practical salinity unit) conditions that are projected to occur in the region by the end of this century as a result of climate change. We used transcriptome analysis (RNA-seq) to determine the gene expression patterns associated with hyposalinity acclimation, and examined the variation in these patterns between the sampled populations. Results Hyposalinity induced different responses in the two populations: in one, only 26 genes were differentially expressed between salinity treatments, while the other population demonstrated up- or downregulation in 3072 genes. In the latter population, the projected future hyposalinity induced an acute response in terms of antioxidant production. Genes associated with membrane composition and structure were also heavily involved, with the upregulation of fatty acid and actin production, and the downregulation of ion channels and alginate pathways. Changes in gene expression patterns clearly indicated an inhibition of the photosynthetic machinery, with a consequent downregulation of carbohydrate production. Simultaneously, energy consumption increased, as revealed by the upregulation of genes associated with respiration and ATP synthesis. Overall, the genes that demonstrated the largest increase in expression were ribosomal proteins involved in translation pathways. The fixation rate of SNP:s was higher within genes responding to hyposalinity than elsewhere in the transcriptome. Conclusions The high fixation rate in the genes coding for salinity acclimation ... Other/Unknown Material North Atlantic University of Turku: UTUPub
institution Open Polar
collection University of Turku: UTUPub
op_collection_id ftunivturku
language English
description Background Rockweeds are among the most important foundation species of temperate rocky littoral shores. In the Baltic Sea, the rockweed Fucus vesiculosus is distributed along a decreasing salinity gradient from the North Atlantic entrance to the low-salinity regions in the north-eastern margins, thus, demonstrating a remarkable tolerance to hyposalinity. The underlying mechanisms for this tolerance are still poorly understood. Here, we exposed F. vesiculosus from two range-margin populations to the hyposaline (2.5 PSU - practical salinity unit) conditions that are projected to occur in the region by the end of this century as a result of climate change. We used transcriptome analysis (RNA-seq) to determine the gene expression patterns associated with hyposalinity acclimation, and examined the variation in these patterns between the sampled populations. Results Hyposalinity induced different responses in the two populations: in one, only 26 genes were differentially expressed between salinity treatments, while the other population demonstrated up- or downregulation in 3072 genes. In the latter population, the projected future hyposalinity induced an acute response in terms of antioxidant production. Genes associated with membrane composition and structure were also heavily involved, with the upregulation of fatty acid and actin production, and the downregulation of ion channels and alginate pathways. Changes in gene expression patterns clearly indicated an inhibition of the photosynthetic machinery, with a consequent downregulation of carbohydrate production. Simultaneously, energy consumption increased, as revealed by the upregulation of genes associated with respiration and ATP synthesis. Overall, the genes that demonstrated the largest increase in expression were ribosomal proteins involved in translation pathways. The fixation rate of SNP:s was higher within genes responding to hyposalinity than elsewhere in the transcriptome. Conclusions The high fixation rate in the genes coding for salinity acclimation ...
author2 ekologia ja evoluutiobiologia, Ecology and Evolutionary Biology
2606402
author Pereyra RT
Rugiu L
Jormalainen V
Panova M
spellingShingle Pereyra RT
Rugiu L
Jormalainen V
Panova M
Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
author_facet Pereyra RT
Rugiu L
Jormalainen V
Panova M
author_sort Pereyra RT
title Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
title_short Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
title_full Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
title_fullStr Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
title_full_unstemmed Gene regulatory response to hyposalinity in the brown seaweed Fucus vesiculosus
title_sort gene regulatory response to hyposalinity in the brown seaweed fucus vesiculosus
publisher BMC
publishDate 2022
url https://www.utupub.fi/handle/10024/156127
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6470-y
genre North Atlantic
genre_facet North Atlantic
op_relation 21
ARTN 42
10.1186/s12864-020-6470-y
BMC Genomics
1
https://www.utupub.fi/handle/10024/156127
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6470-y
URN:NBN:fi-fe2021042822158
1471-2164
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