Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter
The genus Psychrobacter presents an unusually broad range of isolation sources, ranging from the bodies of mammals to extremely cold and saline permafrost soils. In this thesis, I present my investigation of the diversity of Psychrobacter with the goal of identifying differences within the genus whi...
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Universität Tübingen
2021
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Online Access: | http://hdl.handle.net/10900/115579 https://doi.org/10.15496/publikation-56954 http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1155794 |
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ftunivtuebing:oai:publikationen.uni-tuebingen.de:10900/115579 2023-05-15T17:58:00+02:00 Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter Welter, Daphne Kuha Ley, Ruth (Prof. Dr.) 2021-05-28 application/pdf http://hdl.handle.net/10900/115579 https://doi.org/10.15496/publikation-56954 http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1155794 en eng Universität Tübingen 1759143804 http://hdl.handle.net/10900/115579 http://dx.doi.org/10.15496/publikation-56954 http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1155794 ubt-podok http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=de http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=en Genomik Mikrobiologie Evolution Ökologie Phylogenetik 500 ecology bacterial comparative genomics host-microbe interactions phylogenetics pan-genome analysis Dissertation info:eu-repo/semantics/doctoralThesis 2021 ftunivtuebing https://doi.org/10.15496/publikation-56954 2021-05-30T22:41:43Z The genus Psychrobacter presents an unusually broad range of isolation sources, ranging from the bodies of mammals to extremely cold and saline permafrost soils. In this thesis, I present my investigation of the diversity of Psychrobacter with the goal of identifying differences within the genus which may contribute to its surprising ecological distribution. I collected 85 Psychrobacter strains from diverse locations and isolation sources, and approached this problem using genomics, including phylogenomics and pan-genome analysis, as well as ancestral character estimation and analysis of protein coding sequences. I also performed extensive phenotyping, collecting growth curves in 24 different conditions in a gradient of temperature, salt, and nutritional complexity, as well as information on strain bile resistance, oxygen utilization, and for a subset of strains, ability to colonize germ-free mice. I show that Psychrobacter most likely evolved from a Moraxella-like ancestor, that is, a mesophilic pathobiont of mammals, and subsequently diverged into strains that maintain the ability to grow at high temperatures (ie 37 oC) versus those that only grow at cooler temperatures (25 oC or lower). There are some genomic and phenotypic differences amongst Psychrobacter strains based on source of isolation, but ultimately, dividing the genus by their behavior may be more useful. Overall, Psychrobacter promises to be an interesting model group for disentangling niche diversification among close relatives. Doctoral or Postdoctoral Thesis permafrost Eberhard Karls University Tübingen: Publication System |
institution |
Open Polar |
collection |
Eberhard Karls University Tübingen: Publication System |
op_collection_id |
ftunivtuebing |
language |
English |
topic |
Genomik Mikrobiologie Evolution Ökologie Phylogenetik 500 ecology bacterial comparative genomics host-microbe interactions phylogenetics pan-genome analysis |
spellingShingle |
Genomik Mikrobiologie Evolution Ökologie Phylogenetik 500 ecology bacterial comparative genomics host-microbe interactions phylogenetics pan-genome analysis Welter, Daphne Kuha Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
topic_facet |
Genomik Mikrobiologie Evolution Ökologie Phylogenetik 500 ecology bacterial comparative genomics host-microbe interactions phylogenetics pan-genome analysis |
description |
The genus Psychrobacter presents an unusually broad range of isolation sources, ranging from the bodies of mammals to extremely cold and saline permafrost soils. In this thesis, I present my investigation of the diversity of Psychrobacter with the goal of identifying differences within the genus which may contribute to its surprising ecological distribution. I collected 85 Psychrobacter strains from diverse locations and isolation sources, and approached this problem using genomics, including phylogenomics and pan-genome analysis, as well as ancestral character estimation and analysis of protein coding sequences. I also performed extensive phenotyping, collecting growth curves in 24 different conditions in a gradient of temperature, salt, and nutritional complexity, as well as information on strain bile resistance, oxygen utilization, and for a subset of strains, ability to colonize germ-free mice. I show that Psychrobacter most likely evolved from a Moraxella-like ancestor, that is, a mesophilic pathobiont of mammals, and subsequently diverged into strains that maintain the ability to grow at high temperatures (ie 37 oC) versus those that only grow at cooler temperatures (25 oC or lower). There are some genomic and phenotypic differences amongst Psychrobacter strains based on source of isolation, but ultimately, dividing the genus by their behavior may be more useful. Overall, Psychrobacter promises to be an interesting model group for disentangling niche diversification among close relatives. |
author2 |
Ley, Ruth (Prof. Dr.) |
format |
Doctoral or Postdoctoral Thesis |
author |
Welter, Daphne Kuha |
author_facet |
Welter, Daphne Kuha |
author_sort |
Welter, Daphne Kuha |
title |
Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
title_short |
Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
title_full |
Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
title_fullStr |
Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
title_full_unstemmed |
Insights into the ecology and evolutionary history of the bacterial genus Psychrobacter |
title_sort |
insights into the ecology and evolutionary history of the bacterial genus psychrobacter |
publisher |
Universität Tübingen |
publishDate |
2021 |
url |
http://hdl.handle.net/10900/115579 https://doi.org/10.15496/publikation-56954 http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1155794 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
1759143804 http://hdl.handle.net/10900/115579 http://dx.doi.org/10.15496/publikation-56954 http://nbn-resolving.de/urn:nbn:de:bsz:21-dspace-1155794 |
op_rights |
ubt-podok http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=de http://tobias-lib.uni-tuebingen.de/doku/lic_mit_pod.php?la=en |
op_doi |
https://doi.org/10.15496/publikation-56954 |
_version_ |
1766166516353466368 |