Global distribution of a chlorophyll f cyanobacterial marker.
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging fr...
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ftunivtsydney:oai:opus.lib.uts.edu.au:10453/144678 2023-05-15T13:48:10+02:00 Global distribution of a chlorophyll f cyanobacterial marker. Antonaru LA Cardona T Larkum AWD Nürnberg DJ 2020-12-14T05:02:29Z Print-Electronic application/pdf http://hdl.handle.net/10453/144678 eng eng Springer Science and Business Media LLC The ISME journal 10.1038/s41396-020-0670-y The ISME journal, 2020, 14, (9), pp. 2275-2287 1751-7362 1751-7370 http://hdl.handle.net/10453/144678 info:eu-repo/semantics/restrictedAccess 05 Environmental Sciences 06 Biological Sciences 10 Technology Microbiology Journal Article 2020 ftunivtsydney 2022-03-13T13:56:25Z Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype's distribution. Article in Journal/Newspaper Antarc* Antarctic Arctic University of Technology Sydney: OPUS - Open Publications of UTS Scholars Antarctic Arctic |
institution |
Open Polar |
collection |
University of Technology Sydney: OPUS - Open Publications of UTS Scholars |
op_collection_id |
ftunivtsydney |
language |
English |
topic |
05 Environmental Sciences 06 Biological Sciences 10 Technology Microbiology |
spellingShingle |
05 Environmental Sciences 06 Biological Sciences 10 Technology Microbiology Antonaru LA Cardona T Larkum AWD Nürnberg DJ Global distribution of a chlorophyll f cyanobacterial marker. |
topic_facet |
05 Environmental Sciences 06 Biological Sciences 10 Technology Microbiology |
description |
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype's distribution. |
format |
Article in Journal/Newspaper |
author |
Antonaru LA Cardona T Larkum AWD Nürnberg DJ |
author_facet |
Antonaru LA Cardona T Larkum AWD Nürnberg DJ |
author_sort |
Antonaru LA |
title |
Global distribution of a chlorophyll f cyanobacterial marker. |
title_short |
Global distribution of a chlorophyll f cyanobacterial marker. |
title_full |
Global distribution of a chlorophyll f cyanobacterial marker. |
title_fullStr |
Global distribution of a chlorophyll f cyanobacterial marker. |
title_full_unstemmed |
Global distribution of a chlorophyll f cyanobacterial marker. |
title_sort |
global distribution of a chlorophyll f cyanobacterial marker. |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://hdl.handle.net/10453/144678 |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
The ISME journal 10.1038/s41396-020-0670-y The ISME journal, 2020, 14, (9), pp. 2275-2287 1751-7362 1751-7370 http://hdl.handle.net/10453/144678 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1766248812935905280 |