Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event
© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number...
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ftunivtsydney:oai:opus.lib.uts.edu.au:10453/134230 2023-05-15T15:58:50+02:00 Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event King, WL Jenkins, C Go, J Siboni, N Seymour, JR Labbate, M 2019-02-15 application/pdf http://hdl.handle.net/10453/134230 unknown http://purl.org/au-research/grants/arc/LP160101795 Microbial Ecology 10.1007/s00248-018-1226-9 Microbial Ecology, 2019, 77 (2), pp. 502 - 512 0095-3628 http://hdl.handle.net/10453/134230 Microbiology Animals Bacteria Vibrio DNA Viruses Animal Diseases DNA Bacterial RNA Ribosomal 16S Seasons Disease Outbreaks Australia Ostreidae Crassostrea Microbiota Journal Article 2019 ftunivtsydney 2022-03-13T13:51:05Z © 2018, Springer Science+Business Media, LLC, part of Springer Nature. The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number of infectious agents have been associated with these summer mortality events, including viruses (particularly Ostreid herpesvirus 1, OsHV-1) and bacteria; however, cases where no known aetiological agent can be identified are common. In this study, we examined the microbiome of disease-affected and disease-unaffected C. gigas during a 2013–2014 summer mortality event in Port Stephens (Australia) where known oyster pathogens including OsHV-1 were not detected. The adductor muscle microbiomes of 70 C. gigas samples across 12 study sites in the Port Stephens estuary were characterised using 16S rRNA (V1–V3 region) amplicon sequencing, with the aim of comparing the influence of spatial location and disease state on the oyster microbiome. Spatial location was found to be a significant determinant of the disease-affected oyster microbiome. Furthermore, microbiome comparisons between disease states identified a significant increase in rare operational taxonomic units (OTUs) belonging to Vibrio harveyi and an unidentified member of the Vibrio genus in the disease-affected microbiome. This is indicative of a potential role of Vibrio species in oyster disease and supportive of previous culture-based examination of this mortality event. Article in Journal/Newspaper Crassostrea gigas Pacific oyster University of Technology Sydney: OPUS - Open Publications of UTS Scholars Pacific Port Stephens ENVELOPE(-129.689,-129.689,53.332,53.332) |
institution |
Open Polar |
collection |
University of Technology Sydney: OPUS - Open Publications of UTS Scholars |
op_collection_id |
ftunivtsydney |
language |
unknown |
topic |
Microbiology Animals Bacteria Vibrio DNA Viruses Animal Diseases DNA Bacterial RNA Ribosomal 16S Seasons Disease Outbreaks Australia Ostreidae Crassostrea Microbiota |
spellingShingle |
Microbiology Animals Bacteria Vibrio DNA Viruses Animal Diseases DNA Bacterial RNA Ribosomal 16S Seasons Disease Outbreaks Australia Ostreidae Crassostrea Microbiota King, WL Jenkins, C Go, J Siboni, N Seymour, JR Labbate, M Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
topic_facet |
Microbiology Animals Bacteria Vibrio DNA Viruses Animal Diseases DNA Bacterial RNA Ribosomal 16S Seasons Disease Outbreaks Australia Ostreidae Crassostrea Microbiota |
description |
© 2018, Springer Science+Business Media, LLC, part of Springer Nature. The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number of infectious agents have been associated with these summer mortality events, including viruses (particularly Ostreid herpesvirus 1, OsHV-1) and bacteria; however, cases where no known aetiological agent can be identified are common. In this study, we examined the microbiome of disease-affected and disease-unaffected C. gigas during a 2013–2014 summer mortality event in Port Stephens (Australia) where known oyster pathogens including OsHV-1 were not detected. The adductor muscle microbiomes of 70 C. gigas samples across 12 study sites in the Port Stephens estuary were characterised using 16S rRNA (V1–V3 region) amplicon sequencing, with the aim of comparing the influence of spatial location and disease state on the oyster microbiome. Spatial location was found to be a significant determinant of the disease-affected oyster microbiome. Furthermore, microbiome comparisons between disease states identified a significant increase in rare operational taxonomic units (OTUs) belonging to Vibrio harveyi and an unidentified member of the Vibrio genus in the disease-affected microbiome. This is indicative of a potential role of Vibrio species in oyster disease and supportive of previous culture-based examination of this mortality event. |
format |
Article in Journal/Newspaper |
author |
King, WL Jenkins, C Go, J Siboni, N Seymour, JR Labbate, M |
author_facet |
King, WL Jenkins, C Go, J Siboni, N Seymour, JR Labbate, M |
author_sort |
King, WL |
title |
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
title_short |
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
title_full |
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
title_fullStr |
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
title_full_unstemmed |
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event |
title_sort |
characterisation of the pacific oyster microbiome during a summer mortality event |
publishDate |
2019 |
url |
http://hdl.handle.net/10453/134230 |
long_lat |
ENVELOPE(-129.689,-129.689,53.332,53.332) |
geographic |
Pacific Port Stephens |
geographic_facet |
Pacific Port Stephens |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://purl.org/au-research/grants/arc/LP160101795 Microbial Ecology 10.1007/s00248-018-1226-9 Microbial Ecology, 2019, 77 (2), pp. 502 - 512 0095-3628 http://hdl.handle.net/10453/134230 |
_version_ |
1766394607840526336 |