The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics
Heterotrophic marine bacteria play key roles in remineralizing organic matter generated from primary production. However, far more is known about which groups are dominant than about the cellular processes they perform in order to become dominant. In the Southern Ocean, eukaryotic phytoplankton are...
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ftunivtsydney:oai:opus.lib.uts.edu.au:10453/117634 2023-05-15T13:52:22+02:00 The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics Williams, TJ Wilkins, D Long, E Evans, F Demaere, MZ Raftery, MJ Cavicchioli, R 2013-05-01 application/pdf http://hdl.handle.net/10453/117634 unknown Environmental Microbiology 10.1111/1462-2920.12017 Environmental Microbiology, 2013, 15 (5), pp. 1302 - 1317 1462-2912 http://hdl.handle.net/10453/117634 Microbiology Plankton Phytoplankton Bacteria Flavobacteriaceae Proteobacteria Archaea Chlorophyll Proteomics Biodiversity Seawater Phylogeny Antarctic Regions Heterotrophic Processes Metagenomics Eukaryota Chlorophyll A Journal Article 2013 ftunivtsydney 2022-03-13T13:48:09Z Heterotrophic marine bacteria play key roles in remineralizing organic matter generated from primary production. However, far more is known about which groups are dominant than about the cellular processes they perform in order to become dominant. In the Southern Ocean, eukaryotic phytoplankton are the dominant primary producers. In this study we used metagenomics and metaproteomics to determine how the dominant bacterial and archaeal plankton processed bloom material. We examined the microbial community composition in 14 metagenomes and found that the relative abundance of Flavobacteria (dominated by Polaribacter) was positively correlated with chlorophyll a fluorescence, and the relative abundance of SAR11 was inversely correlated with both fluorescence and Flavobacteria abundance. By performing metaproteomics on the sample with the highest relative abundance of Flavobacteria (Newcomb Bay, East Antarctica) we defined how Flavobacteria attach to and degrade diverse complex organic material, how they make labile compounds available to Alphaproteobacteria (especially SAR11) and Gammaproteobacteria, and how these heterotrophic Proteobacteria target and utilize these nutrients. The presence of methylotrophic proteins for archaea and bacteria also indicated the importance of metabolic specialists. Overall, the study provides functional data for the microbial mechanisms of nutrient cycling at the surface of the coastal Southern Ocean. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd. Article in Journal/Newspaper Antarc* Antarctic Antarctica East Antarctica Southern Ocean University of Technology Sydney: OPUS - Open Publications of UTS Scholars Antarctic East Antarctica Newcomb Bay ENVELOPE(110.533,110.533,-66.267,-66.267) Southern Ocean |
institution |
Open Polar |
collection |
University of Technology Sydney: OPUS - Open Publications of UTS Scholars |
op_collection_id |
ftunivtsydney |
language |
unknown |
topic |
Microbiology Plankton Phytoplankton Bacteria Flavobacteriaceae Proteobacteria Archaea Chlorophyll Proteomics Biodiversity Seawater Phylogeny Antarctic Regions Heterotrophic Processes Metagenomics Eukaryota Chlorophyll A |
spellingShingle |
Microbiology Plankton Phytoplankton Bacteria Flavobacteriaceae Proteobacteria Archaea Chlorophyll Proteomics Biodiversity Seawater Phylogeny Antarctic Regions Heterotrophic Processes Metagenomics Eukaryota Chlorophyll A Williams, TJ Wilkins, D Long, E Evans, F Demaere, MZ Raftery, MJ Cavicchioli, R The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
topic_facet |
Microbiology Plankton Phytoplankton Bacteria Flavobacteriaceae Proteobacteria Archaea Chlorophyll Proteomics Biodiversity Seawater Phylogeny Antarctic Regions Heterotrophic Processes Metagenomics Eukaryota Chlorophyll A |
description |
Heterotrophic marine bacteria play key roles in remineralizing organic matter generated from primary production. However, far more is known about which groups are dominant than about the cellular processes they perform in order to become dominant. In the Southern Ocean, eukaryotic phytoplankton are the dominant primary producers. In this study we used metagenomics and metaproteomics to determine how the dominant bacterial and archaeal plankton processed bloom material. We examined the microbial community composition in 14 metagenomes and found that the relative abundance of Flavobacteria (dominated by Polaribacter) was positively correlated with chlorophyll a fluorescence, and the relative abundance of SAR11 was inversely correlated with both fluorescence and Flavobacteria abundance. By performing metaproteomics on the sample with the highest relative abundance of Flavobacteria (Newcomb Bay, East Antarctica) we defined how Flavobacteria attach to and degrade diverse complex organic material, how they make labile compounds available to Alphaproteobacteria (especially SAR11) and Gammaproteobacteria, and how these heterotrophic Proteobacteria target and utilize these nutrients. The presence of methylotrophic proteins for archaea and bacteria also indicated the importance of metabolic specialists. Overall, the study provides functional data for the microbial mechanisms of nutrient cycling at the surface of the coastal Southern Ocean. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd. |
format |
Article in Journal/Newspaper |
author |
Williams, TJ Wilkins, D Long, E Evans, F Demaere, MZ Raftery, MJ Cavicchioli, R |
author_facet |
Williams, TJ Wilkins, D Long, E Evans, F Demaere, MZ Raftery, MJ Cavicchioli, R |
author_sort |
Williams, TJ |
title |
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
title_short |
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
title_full |
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
title_fullStr |
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
title_full_unstemmed |
The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics |
title_sort |
role of planktonic flavobacteria in processing algal organic matter in coastal east antarctica revealed using metagenomics and metaproteomics |
publishDate |
2013 |
url |
http://hdl.handle.net/10453/117634 |
long_lat |
ENVELOPE(110.533,110.533,-66.267,-66.267) |
geographic |
Antarctic East Antarctica Newcomb Bay Southern Ocean |
geographic_facet |
Antarctic East Antarctica Newcomb Bay Southern Ocean |
genre |
Antarc* Antarctic Antarctica East Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Southern Ocean |
op_relation |
Environmental Microbiology 10.1111/1462-2920.12017 Environmental Microbiology, 2013, 15 (5), pp. 1302 - 1317 1462-2912 http://hdl.handle.net/10453/117634 |
_version_ |
1766256644174381056 |