Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi
© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of t...
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ftunivtsydney:oai:opus.lib.uts.edu.au:10453/115456 2023-05-15T13:48:10+02:00 Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi Williams, TJ Liao, Y Ye, J Kuchel, RP Poljak, A Raftery, MJ Cavicchioli, R 2017-06-01 application/pdf http://hdl.handle.net/10453/115456 unknown Environmental Microbiology 10.1111/1462-2920.13705 Environmental Microbiology, 2017, 19 (6), pp. 2210 - 2227 1462-2912 http://hdl.handle.net/10453/115456 Microbiology Cell Membrane Biofilms Membrane Proteins RNA Proteomics Adaptation Physiological DNA Repair Glycosylation Antarctic Regions HSP20 Heat-Shock Proteins Polyhydroxyalkanoates Cold Temperature Halorubrum Lakes Journal Article 2017 ftunivtsydney 2022-03-13T13:58:22Z © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake. Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Technology Sydney: OPUS - Open Publications of UTS Scholars Antarctic The Antarctic |
institution |
Open Polar |
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University of Technology Sydney: OPUS - Open Publications of UTS Scholars |
op_collection_id |
ftunivtsydney |
language |
unknown |
topic |
Microbiology Cell Membrane Biofilms Membrane Proteins RNA Proteomics Adaptation Physiological DNA Repair Glycosylation Antarctic Regions HSP20 Heat-Shock Proteins Polyhydroxyalkanoates Cold Temperature Halorubrum Lakes |
spellingShingle |
Microbiology Cell Membrane Biofilms Membrane Proteins RNA Proteomics Adaptation Physiological DNA Repair Glycosylation Antarctic Regions HSP20 Heat-Shock Proteins Polyhydroxyalkanoates Cold Temperature Halorubrum Lakes Williams, TJ Liao, Y Ye, J Kuchel, RP Poljak, A Raftery, MJ Cavicchioli, R Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
topic_facet |
Microbiology Cell Membrane Biofilms Membrane Proteins RNA Proteomics Adaptation Physiological DNA Repair Glycosylation Antarctic Regions HSP20 Heat-Shock Proteins Polyhydroxyalkanoates Cold Temperature Halorubrum Lakes |
description |
© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Halohasta litchfieldiae represents ∼ 44% and Halorubrum lacusprofundi ∼ 10% of the hypersaline, perennially cold (≥ −20°C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30°C) and low (10 and 4°C) temperatures. The proteomic data indicate that both species responded to low temperature by modifying their cell envelope including protein N-glycosylation, maintaining osmotic balance and translation initiation, and modifying RNA turnover and tRNA modification. Distinctions between the two species included DNA protection and repair strategies (e.g. roles of UspA and Rad50), and metabolism of glycerol and pyruvate. For Hrr. lacusprofundi, low temperature led to the formation of polyhydroxyalkanoate-like granules, with granule formation occurring by an unknown mechanism. Hrr. lacusprofundi also formed biofilms and synthesized high levels of Hsp20 chaperones. Hht. litchfieldiae was characterized by an active CRISPR system, and elevated levels of the core gene expression machinery, which contrasted markedly to the decreased levels of Hrr. lacusprofundi. These findings greatly expand the understanding of cellular mechanisms of cold adaptation in psychrophilic archaea, and provide insight into how Hht. litchfieldiae gains dominance in Deep Lake. |
format |
Article in Journal/Newspaper |
author |
Williams, TJ Liao, Y Ye, J Kuchel, RP Poljak, A Raftery, MJ Cavicchioli, R |
author_facet |
Williams, TJ Liao, Y Ye, J Kuchel, RP Poljak, A Raftery, MJ Cavicchioli, R |
author_sort |
Williams, TJ |
title |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_short |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_full |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_fullStr |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_full_unstemmed |
Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi |
title_sort |
cold adaptation of the antarctic haloarchaea halohasta litchfieldiae and halorubrum lacusprofundi |
publishDate |
2017 |
url |
http://hdl.handle.net/10453/115456 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Environmental Microbiology 10.1111/1462-2920.13705 Environmental Microbiology, 2017, 19 (6), pp. 2210 - 2227 1462-2912 http://hdl.handle.net/10453/115456 |
_version_ |
1766248813165543424 |