Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1
Published version. Source at http://doi.org/10.1016/j.virusres.2014.11.018 . Infectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic pr...
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Online Access: | https://hdl.handle.net/10037/8966 https://doi.org/10.1016/j.virusres.2014.11.018 |
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ftunivtroemsoe:oai:munin.uit.no:10037/8966 2023-05-15T15:30:57+02:00 Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 Lauksund, Reidunn Silje Greiner-Tollersrud, Linn Chang, Chia Jung Robertsen, Børre 2015 https://hdl.handle.net/10037/8966 https://doi.org/10.1016/j.virusres.2014.11.018 eng eng Elsevier Virus Research 2015, 196:113-121 FRIDAID 1224294 doi:10.1016/j.virusres.2014.11.018 0168-1702 https://hdl.handle.net/10037/8966 URN:NBN:no-uit_munin_8531 openAccess IPNV Interferon Antagonism IPS-1 IRF1 IRF3 VDP::Medisinske Fag: 700 Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.1016/j.virusres.2014.11.018 2021-06-25T17:54:37Z Published version. Source at http://doi.org/10.1016/j.virusres.2014.11.018 . Infectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic properties against type I IFN induction in Atlantic salmon. Each of the five IPNV genes cloned into an expression vector were tested for the ability to influence activation of the Atlantic salmon IFNa1 promoter by the interferon promoter inducing protein one (IPS-1) or interferon regulatory factors (IRF). This showed that preVP2,VP3 andVP5 inhibited activation of both promoters, whileVP4 only antagonized activation of the IFNa1 promoter. The viral protease VP4 was the most potent inhibitor of IFN induction, apparently targeting the IRF1 and IRF3 branch of the signaling cascade. VP4 antagonism is independent of its protease activity since the catalytically dead mutant VP4K674A inhibited activation of the IFNa1 promoter to a similar extent as wild type VP4. In contrast to the other IPNV proteins, the RNA-dependent RNA polymerase VP1 activated the IFNa1 promoter. The ability to activate the IFN response was disrupted in the mutant VP1S163A, which has lost the ability to produce dsRNA. VP1 also exhibited synergistic effects with IRF1 and IRF3 in inducing an IFNa1-dependent antiviral state in cells. Taken together these results suggest that IPNV has developed multiple IFN antagonistic properties to prevent IFN-induction by VP1 and its dsRNA genome. Article in Journal/Newspaper Atlantic salmon University of Tromsø: Munin Open Research Archive Virus Research 196 113 121 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
IPNV Interferon Antagonism IPS-1 IRF1 IRF3 VDP::Medisinske Fag: 700 |
spellingShingle |
IPNV Interferon Antagonism IPS-1 IRF1 IRF3 VDP::Medisinske Fag: 700 Lauksund, Reidunn Silje Greiner-Tollersrud, Linn Chang, Chia Jung Robertsen, Børre Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
topic_facet |
IPNV Interferon Antagonism IPS-1 IRF1 IRF3 VDP::Medisinske Fag: 700 |
description |
Published version. Source at http://doi.org/10.1016/j.virusres.2014.11.018 . Infectious pancreatic necrosis virus (IPNV) is one of the major viral pathogens causing disease in farmed Atlantic salmon worldwide. In the present work we show that several of the IPN proteins have powerful antagonistic properties against type I IFN induction in Atlantic salmon. Each of the five IPNV genes cloned into an expression vector were tested for the ability to influence activation of the Atlantic salmon IFNa1 promoter by the interferon promoter inducing protein one (IPS-1) or interferon regulatory factors (IRF). This showed that preVP2,VP3 andVP5 inhibited activation of both promoters, whileVP4 only antagonized activation of the IFNa1 promoter. The viral protease VP4 was the most potent inhibitor of IFN induction, apparently targeting the IRF1 and IRF3 branch of the signaling cascade. VP4 antagonism is independent of its protease activity since the catalytically dead mutant VP4K674A inhibited activation of the IFNa1 promoter to a similar extent as wild type VP4. In contrast to the other IPNV proteins, the RNA-dependent RNA polymerase VP1 activated the IFNa1 promoter. The ability to activate the IFN response was disrupted in the mutant VP1S163A, which has lost the ability to produce dsRNA. VP1 also exhibited synergistic effects with IRF1 and IRF3 in inducing an IFNa1-dependent antiviral state in cells. Taken together these results suggest that IPNV has developed multiple IFN antagonistic properties to prevent IFN-induction by VP1 and its dsRNA genome. |
format |
Article in Journal/Newspaper |
author |
Lauksund, Reidunn Silje Greiner-Tollersrud, Linn Chang, Chia Jung Robertsen, Børre |
author_facet |
Lauksund, Reidunn Silje Greiner-Tollersrud, Linn Chang, Chia Jung Robertsen, Børre |
author_sort |
Lauksund, Reidunn Silje |
title |
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
title_short |
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
title_full |
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
title_fullStr |
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
title_full_unstemmed |
Infectious pancreatic necrosis virus proteins VP2, VP3, VP4 and VP5 antagonize IFNa1 promoter activation while VP1 induces IFNa1 |
title_sort |
infectious pancreatic necrosis virus proteins vp2, vp3, vp4 and vp5 antagonize ifna1 promoter activation while vp1 induces ifna1 |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8966 https://doi.org/10.1016/j.virusres.2014.11.018 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Virus Research 2015, 196:113-121 FRIDAID 1224294 doi:10.1016/j.virusres.2014.11.018 0168-1702 https://hdl.handle.net/10037/8966 URN:NBN:no-uit_munin_8531 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.virusres.2014.11.018 |
container_title |
Virus Research |
container_volume |
196 |
container_start_page |
113 |
op_container_end_page |
121 |
_version_ |
1766361427403079680 |