Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes
Exosomes are secreted nanosize vesicles (30−100 nm) derived from multivesicular endosomes. Exosomes are released by different immune cell types, including T- and B-lymphocytes, mast cells and antigen-presenting cells (APCs). The composition of exosomes - including protein and RNA content reflects th...
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ftunivtroemsoe:oai:munin.uit.no:10037/8888 2023-05-15T15:32:25+02:00 Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes Strandskog, Guro Sobhkhez, Mehrdad Jørgensen, Jorunn B Iliev, Dimitar Borisov 2015-06-28 https://hdl.handle.net/10037/8888 eng eng ISDCI, Murcia ISDCI Congress 2015, 28.06.2015-03.07.2015 FRIDAID 1324171 https://hdl.handle.net/10037/8888 URN:NBN:no-uit_munin_8444 openAccess VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Conference object Konferansebidrag 2015 ftunivtroemsoe 2021-06-25T17:54:32Z Exosomes are secreted nanosize vesicles (30−100 nm) derived from multivesicular endosomes. Exosomes are released by different immune cell types, including T- and B-lymphocytes, mast cells and antigen-presenting cells (APCs). The composition of exosomes - including protein and RNA content reflects their endosomal origin and the type of cells that produce them. Mammalian APCs produce large amounts of exosomes loaded with MHC class I and II molecules with important immunomodulatory properties. In the current study, exosomes were isolated from salmon head kidney leukocytes stimulated with CpG oligonucleotides and yeast beta-glucan (BG) and their protein composition was studied using Western blotting and LC-MS/MS analysis. Major exosome markers were detected including flotillin, CD63, CD81, CD9 and MHC-I and II molecules. Ontology analysis indicated that the exosome samples were enriched in ribosomal, membrane-associated as well as nuclear proteins the last of which reflects the abundance of histones within exosome preparations. Interestingly, label-free MS quantitation suggests that the exosomes might be derived from cross-presenting compartments as they are also enriched in proteins involved in trafficking and loading of MHC-I with exogenous antigens. Conference Object Atlantic salmon Salmo salar University of Tromsø: Munin Open Research Archive |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 Strandskog, Guro Sobhkhez, Mehrdad Jørgensen, Jorunn B Iliev, Dimitar Borisov Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920 |
description |
Exosomes are secreted nanosize vesicles (30−100 nm) derived from multivesicular endosomes. Exosomes are released by different immune cell types, including T- and B-lymphocytes, mast cells and antigen-presenting cells (APCs). The composition of exosomes - including protein and RNA content reflects their endosomal origin and the type of cells that produce them. Mammalian APCs produce large amounts of exosomes loaded with MHC class I and II molecules with important immunomodulatory properties. In the current study, exosomes were isolated from salmon head kidney leukocytes stimulated with CpG oligonucleotides and yeast beta-glucan (BG) and their protein composition was studied using Western blotting and LC-MS/MS analysis. Major exosome markers were detected including flotillin, CD63, CD81, CD9 and MHC-I and II molecules. Ontology analysis indicated that the exosome samples were enriched in ribosomal, membrane-associated as well as nuclear proteins the last of which reflects the abundance of histones within exosome preparations. Interestingly, label-free MS quantitation suggests that the exosomes might be derived from cross-presenting compartments as they are also enriched in proteins involved in trafficking and loading of MHC-I with exogenous antigens. |
format |
Conference Object |
author |
Strandskog, Guro Sobhkhez, Mehrdad Jørgensen, Jorunn B Iliev, Dimitar Borisov |
author_facet |
Strandskog, Guro Sobhkhez, Mehrdad Jørgensen, Jorunn B Iliev, Dimitar Borisov |
author_sort |
Strandskog, Guro |
title |
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
title_short |
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
title_full |
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
title_fullStr |
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
title_full_unstemmed |
Composition of exosomes derived from Atlantic salmon (Salmo salar) head kidney leukocytes |
title_sort |
composition of exosomes derived from atlantic salmon (salmo salar) head kidney leukocytes |
publisher |
ISDCI, Murcia |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8888 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
ISDCI Congress 2015, 28.06.2015-03.07.2015 FRIDAID 1324171 https://hdl.handle.net/10037/8888 URN:NBN:no-uit_munin_8444 |
op_rights |
openAccess |
_version_ |
1766362915520118784 |