Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549)
Published version. Source at http://doi.org/10.1177/0748233715590914 . Seafood industry workers exhibit increased prevalence of respiratory symptoms due to exposure to bioaerosols containing a mixture of bioactive agents. In this study, a human pulmonary epithelial cell model (A549) was exposed to m...
Published in: | Toxicology and Industrial Health |
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Language: | English |
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Online Access: | https://hdl.handle.net/10037/8860 https://doi.org/10.1177/0748233715590914 |
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ftunivtroemsoe:oai:munin.uit.no:10037/8860 2023-05-15T17:54:37+02:00 Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) Bhagwat, Sampada Satchidanand Larsen, Anett Kristin Seternes, Ole Morten Bang, Berit 2015-07-06 https://hdl.handle.net/10037/8860 https://doi.org/10.1177/0748233715590914 eng eng SAGE Publications Toxicology and industrial health 2015 FRIDAID 1265334 doi:10.1177/0748233715590914 1477-0393 https://hdl.handle.net/10037/8860 URN:NBN:no-uit_munin_8398 openAccess Aerosols air quality asthma endotoxin lungs occupational health VDP::Medisinske Fag: 700::Helsefag: 800 Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.1177/0748233715590914 2021-06-25T17:54:34Z Published version. Source at http://doi.org/10.1177/0748233715590914 . Seafood industry workers exhibit increased prevalence of respiratory symptoms due to exposure to bioaerosols containing a mixture of bioactive agents. In this study, a human pulmonary epithelial cell model (A549) was exposed to mixtures of bacterial lipopolysaccharide (LPS) and protease-activated receptor-2 (PAR-2) agonists H-Ser-Leu-Ile-Gly-Lys-Val-NH2 (SLIGKV-NH2), purified salmon (Salmo salar) trypsin or purified king crab (Paralithodes camtschaticus) trypsin. The inflammatory response was measured based on nuclear factor-kappa B (NF-kB) activation of transcription in a luciferase reporter gene assay and interleukin 8 (IL-8) secretion in an enzyme-linked immunosorbent assay. We observed that mixtures of SLIGKV-NH2 or trypsins with LPS augmented the activation of NF-kB and secretion of IL-8. The effect on IL-8 secretion was synergistic when both trypsins and LPS were used in the lower concentration range. The results demonstrate that exposure to mixtures of agents that are relevant to seafood industry workplaces may lead to increased inflammatory signalling compared with exposure to the individual agents alone. Furthermore, the results indicate that synergism may occur with the combined exposure to seafood trypsins and LPS and is most likely to occur when exposure to either agent is low. Article in Journal/Newspaper Paralithodes camtschaticus Salmo salar University of Tromsø: Munin Open Research Archive Toxicology and Industrial Health 32 11 1866 1874 |
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Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Aerosols air quality asthma endotoxin lungs occupational health VDP::Medisinske Fag: 700::Helsefag: 800 |
spellingShingle |
Aerosols air quality asthma endotoxin lungs occupational health VDP::Medisinske Fag: 700::Helsefag: 800 Bhagwat, Sampada Satchidanand Larsen, Anett Kristin Seternes, Ole Morten Bang, Berit Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
topic_facet |
Aerosols air quality asthma endotoxin lungs occupational health VDP::Medisinske Fag: 700::Helsefag: 800 |
description |
Published version. Source at http://doi.org/10.1177/0748233715590914 . Seafood industry workers exhibit increased prevalence of respiratory symptoms due to exposure to bioaerosols containing a mixture of bioactive agents. In this study, a human pulmonary epithelial cell model (A549) was exposed to mixtures of bacterial lipopolysaccharide (LPS) and protease-activated receptor-2 (PAR-2) agonists H-Ser-Leu-Ile-Gly-Lys-Val-NH2 (SLIGKV-NH2), purified salmon (Salmo salar) trypsin or purified king crab (Paralithodes camtschaticus) trypsin. The inflammatory response was measured based on nuclear factor-kappa B (NF-kB) activation of transcription in a luciferase reporter gene assay and interleukin 8 (IL-8) secretion in an enzyme-linked immunosorbent assay. We observed that mixtures of SLIGKV-NH2 or trypsins with LPS augmented the activation of NF-kB and secretion of IL-8. The effect on IL-8 secretion was synergistic when both trypsins and LPS were used in the lower concentration range. The results demonstrate that exposure to mixtures of agents that are relevant to seafood industry workplaces may lead to increased inflammatory signalling compared with exposure to the individual agents alone. Furthermore, the results indicate that synergism may occur with the combined exposure to seafood trypsins and LPS and is most likely to occur when exposure to either agent is low. |
format |
Article in Journal/Newspaper |
author |
Bhagwat, Sampada Satchidanand Larsen, Anett Kristin Seternes, Ole Morten Bang, Berit |
author_facet |
Bhagwat, Sampada Satchidanand Larsen, Anett Kristin Seternes, Ole Morten Bang, Berit |
author_sort |
Bhagwat, Sampada Satchidanand |
title |
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
title_short |
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
title_full |
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
title_fullStr |
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
title_full_unstemmed |
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549) |
title_sort |
mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (a549) |
publisher |
SAGE Publications |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8860 https://doi.org/10.1177/0748233715590914 |
genre |
Paralithodes camtschaticus Salmo salar |
genre_facet |
Paralithodes camtschaticus Salmo salar |
op_relation |
Toxicology and industrial health 2015 FRIDAID 1265334 doi:10.1177/0748233715590914 1477-0393 https://hdl.handle.net/10037/8860 URN:NBN:no-uit_munin_8398 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1177/0748233715590914 |
container_title |
Toxicology and Industrial Health |
container_volume |
32 |
container_issue |
11 |
container_start_page |
1866 |
op_container_end_page |
1874 |
_version_ |
1766162418854002688 |