Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses
Published version. Also available at http://dx.doi.org/10.3389/fmicb.2015.00399 The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal...
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Online Access: | https://hdl.handle.net/10037/8767 https://doi.org/10.3389/fmicb.2015.00399 |
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ftunivtroemsoe:oai:munin.uit.no:10037/8767 2023-05-15T14:56:52+02:00 Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Tas, Neslihan Elberling, Bo Jansson, Janet K Semenchuk, Philipp Priemé, Anders 2015-04-30 https://hdl.handle.net/10037/8767 https://doi.org/10.3389/fmicb.2015.00399 eng eng Frontiers Frontiers in Microbiology 2015, 6:399 FRIDAID 1256675 doi:10.3389/fmicb.2015.00399 1664-302X https://hdl.handle.net/10037/8767 URN:NBN:no-uit_munin_8338 openAccess VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 permafrost active layer seasonal variation bacterial community structure 16S rRNA gene Arctic Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.3389/fmicb.2015.00399 2021-06-25T17:54:39Z Published version. Also available at http://dx.doi.org/10.3389/fmicb.2015.00399 The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below −10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface. Article in Journal/Newspaper Arctic permafrost Svalbard University of Tromsø: Munin Open Research Archive Arctic Svalbard Frontiers in Microbiology 6 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 permafrost active layer seasonal variation bacterial community structure 16S rRNA gene Arctic |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 permafrost active layer seasonal variation bacterial community structure 16S rRNA gene Arctic Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Tas, Neslihan Elberling, Bo Jansson, Janet K Semenchuk, Philipp Priemé, Anders Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 permafrost active layer seasonal variation bacterial community structure 16S rRNA gene Arctic |
description |
Published version. Also available at http://dx.doi.org/10.3389/fmicb.2015.00399 The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below −10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface. |
format |
Article in Journal/Newspaper |
author |
Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Tas, Neslihan Elberling, Bo Jansson, Janet K Semenchuk, Philipp Priemé, Anders |
author_facet |
Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Tas, Neslihan Elberling, Bo Jansson, Janet K Semenchuk, Philipp Priemé, Anders |
author_sort |
Schostag, Morten |
title |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_short |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_full |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_fullStr |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_full_unstemmed |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_sort |
distinct summer and winter bacterial communities in the active layer of svalbard permafrost revealed by dna- and rna-based analyses |
publisher |
Frontiers |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8767 https://doi.org/10.3389/fmicb.2015.00399 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic permafrost Svalbard |
genre_facet |
Arctic permafrost Svalbard |
op_relation |
Frontiers in Microbiology 2015, 6:399 FRIDAID 1256675 doi:10.3389/fmicb.2015.00399 1664-302X https://hdl.handle.net/10037/8767 URN:NBN:no-uit_munin_8338 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2015.00399 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
_version_ |
1766328925552640000 |