Transcription factor T-bet in Atlantic salmon: characterization and gene expression in mucosal tissues during Aeromonas salmonicida infection

• Published in: Frontiers in Immunology
• Main Authors: Kumari, Jaya, Zhang, Zuobing, Swain, Trilochan, Chi, Heng, Niu, Cuijuan, Bøgwald, Jarl, Dalmo, Roy Ambli
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers 2015
• Subjects: VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923; VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923; Atlantic salmon; T-bet; A. salmonicida; gene expression; mucosal immunity
• Online Access: https://hdl.handle.net/10037/8763; https://doi.org/10.3389/fimmu.2015.00345

Published version. Also available at http://dx.doi.org/10.3389/fimmu.2015.00345 The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.