Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells
License:Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) Background: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipe...
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Online Access: | https://hdl.handle.net/10037/8578 https://doi.org/10.1186/s13028-016-0188-5 |
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ftunivtroemsoe:oai:munin.uit.no:10037/8578 2023-05-15T15:59:53+02:00 Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells Larsen, Anett Kristin Godfroid, Jacques Nymo, Ingebjørg Helena 2016-01-25 https://hdl.handle.net/10037/8578 https://doi.org/10.1186/s13028-016-0188-5 eng eng BioMed Central Acta Veterinaria Scandinavica 2016, 58(9) FRIDAID 1325052 doi:10.1186/s13028-016-0188-5 1751-0147 https://hdl.handle.net/10037/8578 URN:NBN:no-uit_munin_8143 openAccess Brucella pinnipedialis Epithelial cells Hooded seal Infection In vitro Marine ecosystem Pinnipeds Spillover VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 Journal article Tidsskriftartikkel Peer reviewed 2016 ftunivtroemsoe https://doi.org/10.1186/s13028-016-0188-5 2021-06-25T17:54:35Z License:Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) Background: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipeds have been sparse. The capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that B. pinnipedialis spp. are rapidly eliminated from hooded seal (Cystophora cristata) alveolar macrophages. Results: To investigate if multiplication could take place in other hooded seal cell types, primary epithelial cells were isolated, verified to express the epithelial marker cytokeratin and challenged with three different strains of B. pinnipedialis; B. pinnipedialis sp. nov., B. pinnipedialis hooded seal strain B17, and B. pinnipedialis hooded seal strain 22F1. All strains were steadily eliminated and the amounts of intracellular bacteria were reduced to less than one-third by 48 h post infection. Intracellular presence was verified using immunocytochemistry. Conclusions: So far, intracellular multiplication in seal cells has not been documented for B. pinnipedialis. The lack of intracellular survival in macrophages, as well as in epithelial cells, together with the fact that pathological changes due to B. pinnipedialis infection is not yet identified in seals, suggests that the bacteria may only cause a mild, acute and transient infection. These findings also contribute to substantiate the hypothesis that seals may not be the primary host of B. pinnipedialis and that the transmission to seals are caused by other species in the marine environment. Article in Journal/Newspaper Cystophora cristata hooded seal University of Tromsø: Munin Open Research Archive Acta Veterinaria Scandinavica 58 1 |
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University of Tromsø: Munin Open Research Archive |
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ftunivtroemsoe |
language |
English |
topic |
Brucella pinnipedialis Epithelial cells Hooded seal Infection In vitro Marine ecosystem Pinnipeds Spillover VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 |
spellingShingle |
Brucella pinnipedialis Epithelial cells Hooded seal Infection In vitro Marine ecosystem Pinnipeds Spillover VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 Larsen, Anett Kristin Godfroid, Jacques Nymo, Ingebjørg Helena Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
topic_facet |
Brucella pinnipedialis Epithelial cells Hooded seal Infection In vitro Marine ecosystem Pinnipeds Spillover VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural science: 400::Zoology and botany: 480::Marine biology: 497 |
description |
License:Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) Background: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipeds have been sparse. The capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that B. pinnipedialis spp. are rapidly eliminated from hooded seal (Cystophora cristata) alveolar macrophages. Results: To investigate if multiplication could take place in other hooded seal cell types, primary epithelial cells were isolated, verified to express the epithelial marker cytokeratin and challenged with three different strains of B. pinnipedialis; B. pinnipedialis sp. nov., B. pinnipedialis hooded seal strain B17, and B. pinnipedialis hooded seal strain 22F1. All strains were steadily eliminated and the amounts of intracellular bacteria were reduced to less than one-third by 48 h post infection. Intracellular presence was verified using immunocytochemistry. Conclusions: So far, intracellular multiplication in seal cells has not been documented for B. pinnipedialis. The lack of intracellular survival in macrophages, as well as in epithelial cells, together with the fact that pathological changes due to B. pinnipedialis infection is not yet identified in seals, suggests that the bacteria may only cause a mild, acute and transient infection. These findings also contribute to substantiate the hypothesis that seals may not be the primary host of B. pinnipedialis and that the transmission to seals are caused by other species in the marine environment. |
format |
Article in Journal/Newspaper |
author |
Larsen, Anett Kristin Godfroid, Jacques Nymo, Ingebjørg Helena |
author_facet |
Larsen, Anett Kristin Godfroid, Jacques Nymo, Ingebjørg Helena |
author_sort |
Larsen, Anett Kristin |
title |
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
title_short |
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
title_full |
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
title_fullStr |
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
title_full_unstemmed |
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells |
title_sort |
brucella pinnipedialis in hooded seal (cystophora cristata) primary epithelial cells |
publisher |
BioMed Central |
publishDate |
2016 |
url |
https://hdl.handle.net/10037/8578 https://doi.org/10.1186/s13028-016-0188-5 |
genre |
Cystophora cristata hooded seal |
genre_facet |
Cystophora cristata hooded seal |
op_relation |
Acta Veterinaria Scandinavica 2016, 58(9) FRIDAID 1325052 doi:10.1186/s13028-016-0188-5 1751-0147 https://hdl.handle.net/10037/8578 URN:NBN:no-uit_munin_8143 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1186/s13028-016-0188-5 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
58 |
container_issue |
1 |
_version_ |
1766395774308974592 |