Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashe...
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ftunivtroemsoe:oai:munin.uit.no:10037/8142 2023-05-15T17:43:33+02:00 Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) Aarnes, Siv Grethe Fløystad, Ida Schregel, Julia Vindstad, Ole Petter L. Jepsen, Jane Uhd Eiken, Hans Geir Ims, Rolf Anker Hagen, Snorre 2015-09-17 https://hdl.handle.net/10037/8142 https://doi.org/10.3390/ijms160922541 eng eng International Journal of Molecular Sciences 16(2015) s. 22541-22554 FRIDAID 1266899 doi:10.3390/ijms160922541 1422-0067 https://hdl.handle.net/10037/8142 URN:NBN:no-uit_munin_7730 openAccess tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.3390/ijms160922541 2021-06-25T17:54:25Z The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. tri- and tetranucleotide microsatellites; multiplex PCR; Lepidoptera; population genetics Article in Journal/Newspaper Northern Norway University of Tromsø: Munin Open Research Archive Norway International Journal of Molecular Sciences 16 9 22541 22554 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 |
spellingShingle |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 Aarnes, Siv Grethe Fløystad, Ida Schregel, Julia Vindstad, Ole Petter L. Jepsen, Jane Uhd Eiken, Hans Geir Ims, Rolf Anker Hagen, Snorre Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
topic_facet |
tri- and tetranucleotide microsatellites multiplex PCR Lepidoptera population genetics VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 VDP::Mathematics and natural science: 400::Basic biosciences: 470::Molecular biology: 473 |
description |
The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. tri- and tetranucleotide microsatellites; multiplex PCR; Lepidoptera; population genetics |
format |
Article in Journal/Newspaper |
author |
Aarnes, Siv Grethe Fløystad, Ida Schregel, Julia Vindstad, Ole Petter L. Jepsen, Jane Uhd Eiken, Hans Geir Ims, Rolf Anker Hagen, Snorre |
author_facet |
Aarnes, Siv Grethe Fløystad, Ida Schregel, Julia Vindstad, Ole Petter L. Jepsen, Jane Uhd Eiken, Hans Geir Ims, Rolf Anker Hagen, Snorre |
author_sort |
Aarnes, Siv Grethe |
title |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_short |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_fullStr |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_full_unstemmed |
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) |
title_sort |
identification and evaluation of 21 novel microsatellite markers from the autumnal moth (epirrita autumnata) (lepidoptera: geometridae) |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8142 https://doi.org/10.3390/ijms160922541 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_relation |
International Journal of Molecular Sciences 16(2015) s. 22541-22554 FRIDAID 1266899 doi:10.3390/ijms160922541 1422-0067 https://hdl.handle.net/10037/8142 URN:NBN:no-uit_munin_7730 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.3390/ijms160922541 |
container_title |
International Journal of Molecular Sciences |
container_volume |
16 |
container_issue |
9 |
container_start_page |
22541 |
op_container_end_page |
22554 |
_version_ |
1766145654513467392 |