Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA
Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Resul...
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BioMed Central
2015
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Online Access: | https://hdl.handle.net/10037/8076 https://doi.org/10.1186/s12866-015-0486-5 |
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ftunivtroemsoe:oai:munin.uit.no:10037/8076 2023-05-15T15:04:34+02:00 Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA Henningsson, Anna J. Hvidsten, Dag Kristiansen, Bjørn Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew 2015-08-01 https://hdl.handle.net/10037/8076 https://doi.org/10.1186/s12866-015-0486-5 eng eng BioMed Central BMC Microbiology (2015) 15:153 FRIDAID 1257977 doi:10.1186/s12866-015-0486-5 1471-2180 https://hdl.handle.net/10037/8076 URN:NBN:no-uit_munin_7664 openAccess Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.1186/s12866-015-0486-5 2021-06-25T17:54:25Z Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Results: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway. Article in Journal/Newspaper Arctic Northern Norway University of Tromsø: Munin Open Research Archive Arctic Norway BMC Microbiology 15 1 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
spellingShingle |
Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Henningsson, Anna J. Hvidsten, Dag Kristiansen, Bjørn Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
topic_facet |
Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence VDP::Mathematics and natural science: 400::Basic biosciences: 470 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
description |
Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Results: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway. |
format |
Article in Journal/Newspaper |
author |
Henningsson, Anna J. Hvidsten, Dag Kristiansen, Bjørn Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew |
author_facet |
Henningsson, Anna J. Hvidsten, Dag Kristiansen, Bjørn Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew |
author_sort |
Henningsson, Anna J. |
title |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_short |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_full |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_fullStr |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_full_unstemmed |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_sort |
detection of anaplasma phagocytophilum in ixodes ricinus ticks from norway using a realtime pcr assay targeting the anaplasma citrate synthase gene glta |
publisher |
BioMed Central |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/8076 https://doi.org/10.1186/s12866-015-0486-5 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic Northern Norway |
genre_facet |
Arctic Northern Norway |
op_relation |
BMC Microbiology (2015) 15:153 FRIDAID 1257977 doi:10.1186/s12866-015-0486-5 1471-2180 https://hdl.handle.net/10037/8076 URN:NBN:no-uit_munin_7664 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1186/s12866-015-0486-5 |
container_title |
BMC Microbiology |
container_volume |
15 |
container_issue |
1 |
_version_ |
1766336316229812224 |