Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA

• Published in: BMC Microbiology
• Main Authors: Henningsson, Anna J., Hvidsten, Dag, Kristiansen, Bjørn Erik, Matussek, Andreas, Stuen, Snorre, Jenkins, Andrew
• Format: Article in Journal/Newspaper
• Language: English
• Published: BioMed Central 2015
• Subjects: Anaplasma phagocytophilum; Ixodes ricinus; TaqMan realtime PCR; Norway; gltA; Prevalence; VDP::Mathematics and natural science: 400::Basic biosciences: 470; VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470; Arctic; Northern Norway
• Online Access: https://hdl.handle.net/10037/8076; https://doi.org/10.1186/s12866-015-0486-5

Background: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Results: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.