Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability
Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic...
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Online Access: | https://hdl.handle.net/10037/7886 https://doi.org/10.1371/journal.pone.0129733 |
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ftunivtroemsoe:oai:munin.uit.no:10037/7886 2023-05-15T14:24:54+02:00 Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil 2015-06-17 https://hdl.handle.net/10037/7886 https://doi.org/10.1371/journal.pone.0129733 eng eng Public Library of Science (PLoS) Blake LI, Tveit A, Øvreås L, Head IM, Gray ND (2015): Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability, PLoS ONE 10(6): e0129733 FRIDAID 1250824 doi:10.1371/journal.pone.0129733 1932-6203 https://hdl.handle.net/10037/7886 URN:NBN:no-uit_munin_7485 openAccess VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Sedimentologi: 456 VDP::Mathematics and natural science: 400::Geosciences: 450::Sedimentology: 456 Journal article Tidsskriftartikkel Peer reviewed 2015 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0129733 2021-06-25T17:54:20Z Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic substrate amendment, (acetate, methanol and H2/CO2) on methanogen activity and methanogen community structure in high Arctic wetlands (Solvatnet and Stuphallet, Svalbard). Methane production was not detected in Stuphallet sediment microcosms (over a 150 day period) and occurred within Solvatnet sediments microcosms (within 24 hours) at temperatures from 5 to 40°C, the maximum temperature being at far higher than in situ maximum temperatures (which range from air temperatures of -1.4 to 14.1°C during summer months). Distinct responses were observed in the Solvatnet methanogen community under different short term incubation conditions. Specifically, different communities were selected at higher and lower temperatures. At lower temperatures (5°C) addition of exogenous substrates (acetate, methanol or H2/CO2) had no stimulatory effect on the rate of methanogenesis or on methanogen community structure. The community in these incubations was dominated by members of the Methanoregulaceae/WCHA2-08 family-level group, which were most similar to the psychrotolerant hydrogenotrophic methanogen Methanosphaerula palustris strain E1-9c. In contrast, at higher temperatures, substrate amendment enhanced methane production in H2/CO2 amended microcosms, and played a clear role in structuring methanogen communities. Specifically, at 30°C members of the Methanoregulaceae/WCHA2-08 predominated following incubation with H2/CO2, and Methanosarcinaceaeand Methanosaetaceae were enriched in response to acetate addition. These results may indicate that in transiently cold environments, methanogen communities can rapidly respond to moderate short term increases in temperature, but not necessarily to the seasonal release of previously frozen organic carbon from thawing permafrost soils. However, as temperatures increase such inputs of carbon will likely have a greater influence on methane production and methanogen community structure. Understanding the action and limitations of anaerobic microorganisms within cold environments may provide information which can be used in defining region-specific differences in the microbial processes; which ultimately control methane flux to the atmosphere. Article in Journal/Newspaper Arctic Arctic permafrost Svalbard University of Tromsø: Munin Open Research Archive Arctic Svalbard PLOS ONE 10 6 e0129733 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Sedimentologi: 456 VDP::Mathematics and natural science: 400::Geosciences: 450::Sedimentology: 456 |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Sedimentologi: 456 VDP::Mathematics and natural science: 400::Geosciences: 450::Sedimentology: 456 Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Sedimentologi: 456 VDP::Mathematics and natural science: 400::Geosciences: 450::Sedimentology: 456 |
description |
Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic substrate amendment, (acetate, methanol and H2/CO2) on methanogen activity and methanogen community structure in high Arctic wetlands (Solvatnet and Stuphallet, Svalbard). Methane production was not detected in Stuphallet sediment microcosms (over a 150 day period) and occurred within Solvatnet sediments microcosms (within 24 hours) at temperatures from 5 to 40°C, the maximum temperature being at far higher than in situ maximum temperatures (which range from air temperatures of -1.4 to 14.1°C during summer months). Distinct responses were observed in the Solvatnet methanogen community under different short term incubation conditions. Specifically, different communities were selected at higher and lower temperatures. At lower temperatures (5°C) addition of exogenous substrates (acetate, methanol or H2/CO2) had no stimulatory effect on the rate of methanogenesis or on methanogen community structure. The community in these incubations was dominated by members of the Methanoregulaceae/WCHA2-08 family-level group, which were most similar to the psychrotolerant hydrogenotrophic methanogen Methanosphaerula palustris strain E1-9c. In contrast, at higher temperatures, substrate amendment enhanced methane production in H2/CO2 amended microcosms, and played a clear role in structuring methanogen communities. Specifically, at 30°C members of the Methanoregulaceae/WCHA2-08 predominated following incubation with H2/CO2, and Methanosarcinaceaeand Methanosaetaceae were enriched in response to acetate addition. These results may indicate that in transiently cold environments, methanogen communities can rapidly respond to moderate short term increases in temperature, but not necessarily to the seasonal release of previously frozen organic carbon from thawing permafrost soils. However, as temperatures increase such inputs of carbon will likely have a greater influence on methane production and methanogen community structure. Understanding the action and limitations of anaerobic microorganisms within cold environments may provide information which can be used in defining region-specific differences in the microbial processes; which ultimately control methane flux to the atmosphere. |
format |
Article in Journal/Newspaper |
author |
Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil |
author_facet |
Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil |
author_sort |
Blake, Lynsay |
title |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_short |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_full |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_fullStr |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_full_unstemmed |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_sort |
response of methanogens in arctic sediments to temperature and methanogenic substrate availability |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://hdl.handle.net/10037/7886 https://doi.org/10.1371/journal.pone.0129733 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Arctic permafrost Svalbard |
genre_facet |
Arctic Arctic permafrost Svalbard |
op_relation |
Blake LI, Tveit A, Øvreås L, Head IM, Gray ND (2015): Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability, PLoS ONE 10(6): e0129733 FRIDAID 1250824 doi:10.1371/journal.pone.0129733 1932-6203 https://hdl.handle.net/10037/7886 URN:NBN:no-uit_munin_7485 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0129733 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
6 |
container_start_page |
e0129733 |
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1766297347834249216 |