Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species
During hypoxic insult, neuronal tissue undergoes an excitotoxic cascade of events leading to cell death. This response involves loss of membrane potential and uncontrolled release of primarily excitatory neurotransmitters. I propose that this fatal cascade is significantly delayed or attenuated in d...
Main Author: | |
---|---|
Format: | Master Thesis |
Language: | English |
Published: |
UiT Norges arktiske universitet
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/10037/6822 |
id |
ftunivtroemsoe:oai:munin.uit.no:10037/6822 |
---|---|
record_format |
openpolar |
spelling |
ftunivtroemsoe:oai:munin.uit.no:10037/6822 2023-05-15T15:55:58+02:00 Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species Ragazzi, Lorenzo 2014-08-15 https://hdl.handle.net/10037/6822 eng eng UiT Norges arktiske universitet UiT The Arctic University of Norway https://hdl.handle.net/10037/6822 URN:NBN:no-uit_munin_6422 openAccess Copyright 2014 The Author(s) VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Hypoxia Diving physiology Eider duck Chicken Birds Glutamate Serine Glycine Threonine Neurophysiology Brain UPLC BIO-3950 Master thesis Mastergradsoppgave 2014 ftunivtroemsoe 2021-06-25T17:54:03Z During hypoxic insult, neuronal tissue undergoes an excitotoxic cascade of events leading to cell death. This response involves loss of membrane potential and uncontrolled release of primarily excitatory neurotransmitters. I propose that this fatal cascade is significantly delayed or attenuated in diving species, which are adapted to repeated exposures to hypoxic conditions as a consequence of breath-hold diving. Here I have compared the in vitro overflow/efflux of neurotransmitters from cerebellar slices of a diving bird, the common eider duck (Somateria mollissima) and of the non-diving chicken (Gallus gallus domesticus), using a microperfusion technique as described by Kirschner et al. (2009). Slices were cut in ice cold oxygenated aCSF and stored at room temperature before transfer to custom-made microperfusion chambers. After 20 min equilibration at 37°C, the superfusate was collected while exposing the slices to 20 min hypoxic or normoxic (control) perfusions, before reoxygenation. I used Waters UPLC AAA application solution for physiological amino acids to detect changes in the concentration of the excitatory neurotransmitters Glutamate and Serine and the inhibitory Glycine, in the superfusate. I found that neurotransmitter release, for both eider duck and chicken, tended to increase in hypoxia. In addition, higher levels of neurotrasmitter efflux during the hypoxia, and normoxia exposure were present in the chicken. Master Thesis Common Eider Somateria mollissima University of Tromsø: Munin Open Research Archive |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Hypoxia Diving physiology Eider duck Chicken Birds Glutamate Serine Glycine Threonine Neurophysiology Brain UPLC BIO-3950 |
spellingShingle |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Hypoxia Diving physiology Eider duck Chicken Birds Glutamate Serine Glycine Threonine Neurophysiology Brain UPLC BIO-3950 Ragazzi, Lorenzo Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
topic_facet |
VDP::Mathematics and natural science: 400::Zoology and botany: 480 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 Hypoxia Diving physiology Eider duck Chicken Birds Glutamate Serine Glycine Threonine Neurophysiology Brain UPLC BIO-3950 |
description |
During hypoxic insult, neuronal tissue undergoes an excitotoxic cascade of events leading to cell death. This response involves loss of membrane potential and uncontrolled release of primarily excitatory neurotransmitters. I propose that this fatal cascade is significantly delayed or attenuated in diving species, which are adapted to repeated exposures to hypoxic conditions as a consequence of breath-hold diving. Here I have compared the in vitro overflow/efflux of neurotransmitters from cerebellar slices of a diving bird, the common eider duck (Somateria mollissima) and of the non-diving chicken (Gallus gallus domesticus), using a microperfusion technique as described by Kirschner et al. (2009). Slices were cut in ice cold oxygenated aCSF and stored at room temperature before transfer to custom-made microperfusion chambers. After 20 min equilibration at 37°C, the superfusate was collected while exposing the slices to 20 min hypoxic or normoxic (control) perfusions, before reoxygenation. I used Waters UPLC AAA application solution for physiological amino acids to detect changes in the concentration of the excitatory neurotransmitters Glutamate and Serine and the inhibitory Glycine, in the superfusate. I found that neurotransmitter release, for both eider duck and chicken, tended to increase in hypoxia. In addition, higher levels of neurotrasmitter efflux during the hypoxia, and normoxia exposure were present in the chicken. |
format |
Master Thesis |
author |
Ragazzi, Lorenzo |
author_facet |
Ragazzi, Lorenzo |
author_sort |
Ragazzi, Lorenzo |
title |
Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
title_short |
Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
title_full |
Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
title_fullStr |
Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
title_full_unstemmed |
Efflux of neurotransmitter from brain slices during O2 deprivation – comparing diving and non-diving species |
title_sort |
efflux of neurotransmitter from brain slices during o2 deprivation – comparing diving and non-diving species |
publisher |
UiT Norges arktiske universitet |
publishDate |
2014 |
url |
https://hdl.handle.net/10037/6822 |
genre |
Common Eider Somateria mollissima |
genre_facet |
Common Eider Somateria mollissima |
op_relation |
https://hdl.handle.net/10037/6822 URN:NBN:no-uit_munin_6422 |
op_rights |
openAccess Copyright 2014 The Author(s) |
_version_ |
1766391441026711552 |