CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes
The Mitogen-activated protein kinases (MAPK) are involved in transmitting intracellular signals downstream of diverse cell surface receptors and mediate the response to ligands such as growth factors, hormones and cytokines. In addition, MAPK are critically involved in the innate immune response to...
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ftunivtroemsoe:oai:munin.uit.no:10037/5884 2023-05-15T18:10:00+02:00 CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes Iliev, Dimitri Borisov Hansen, Tom Egil Jørgensen, Sven Martin Krasnov, Aleksei Jørgensen, Jorunn B 2013 https://hdl.handle.net/10037/5884 http://dx.doi.org710.1016/j.fsi.2013.07.014 eng eng Elsevier Fish and Shellfish Immunology 35(2013) nr. 4 s. 1079-1085 FRIDAID 1072659 http://dx.doi.org710.1016/j.fsi.2013.07.014 1050-4648 https://hdl.handle.net/10037/5884 URN:NBN:no-uit_munin_5578 openAccess VDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478 Journal article Tidsskriftartikkel Peer reviewed 2013 ftunivtroemsoe 2021-06-25T17:53:47Z The Mitogen-activated protein kinases (MAPK) are involved in transmitting intracellular signals downstream of diverse cell surface receptors and mediate the response to ligands such as growth factors, hormones and cytokines. In addition, MAPK are critically involved in the innate immune response to pathogen-derived substances, commonly referred to as pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharide (LPS) and bacterial DNA rich in CpG dinucleotides. Currently, a great deal of knowledge is available about the involvement of MAPK in the innate immune response to PAMPs in mammals; however, little is known about the role of the different MAPK classes in the immune response to PAMPs in lower vertebrates. In the current study, p38 phosphorylation was induced by CpG oligonucleotides (ODNs) and LPS in primary salmon mononuclear phagocytes. Pre-treatment of the cells with a p38 inhibitor (SB203580) blocked the PAMP-induced p38 activity and suppressed the upregulation of most of the CpG- and LPS-induced transcripts highlighting the role of this kinase in the salmon innate immune response to PAMPs. In contrast to p38, the phosphorylation of extracellular signal-regulated kinase (ERK), a MAPK involved primarily in response to mitogens, was high in resting cells and, surprisingly, incubation with both CpG and control ODNs downregulated the phospho-ERK levels independently of p38 activation. The basal phospho-ERK level and the CpG-inducible p38 phosphorylation were greatly influenced by the length of in vitro incubation. The basal phospho-ERK level increased gradually throughout a 5-day culture period and was PI3K-dependent as demonstrated by its sensitivity to Wortmannin suggesting it is influenced by growth factors. Overall these data indicate that both basal and PAMP-induced activity of MAPKs might be greatly influenced by the differentiation status of salmon mononuclear phagocytes. Article in Journal/Newspaper Salmo salar University of Tromsø: Munin Open Research Archive |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478 |
spellingShingle |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478 Iliev, Dimitri Borisov Hansen, Tom Egil Jørgensen, Sven Martin Krasnov, Aleksei Jørgensen, Jorunn B CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
topic_facet |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478 |
description |
The Mitogen-activated protein kinases (MAPK) are involved in transmitting intracellular signals downstream of diverse cell surface receptors and mediate the response to ligands such as growth factors, hormones and cytokines. In addition, MAPK are critically involved in the innate immune response to pathogen-derived substances, commonly referred to as pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharide (LPS) and bacterial DNA rich in CpG dinucleotides. Currently, a great deal of knowledge is available about the involvement of MAPK in the innate immune response to PAMPs in mammals; however, little is known about the role of the different MAPK classes in the immune response to PAMPs in lower vertebrates. In the current study, p38 phosphorylation was induced by CpG oligonucleotides (ODNs) and LPS in primary salmon mononuclear phagocytes. Pre-treatment of the cells with a p38 inhibitor (SB203580) blocked the PAMP-induced p38 activity and suppressed the upregulation of most of the CpG- and LPS-induced transcripts highlighting the role of this kinase in the salmon innate immune response to PAMPs. In contrast to p38, the phosphorylation of extracellular signal-regulated kinase (ERK), a MAPK involved primarily in response to mitogens, was high in resting cells and, surprisingly, incubation with both CpG and control ODNs downregulated the phospho-ERK levels independently of p38 activation. The basal phospho-ERK level and the CpG-inducible p38 phosphorylation were greatly influenced by the length of in vitro incubation. The basal phospho-ERK level increased gradually throughout a 5-day culture period and was PI3K-dependent as demonstrated by its sensitivity to Wortmannin suggesting it is influenced by growth factors. Overall these data indicate that both basal and PAMP-induced activity of MAPKs might be greatly influenced by the differentiation status of salmon mononuclear phagocytes. |
format |
Article in Journal/Newspaper |
author |
Iliev, Dimitri Borisov Hansen, Tom Egil Jørgensen, Sven Martin Krasnov, Aleksei Jørgensen, Jorunn B |
author_facet |
Iliev, Dimitri Borisov Hansen, Tom Egil Jørgensen, Sven Martin Krasnov, Aleksei Jørgensen, Jorunn B |
author_sort |
Iliev, Dimitri Borisov |
title |
CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
title_short |
CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
title_full |
CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
title_fullStr |
CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
title_full_unstemmed |
CpG- and LPS-activated MAPK signaling in in vitro cultured salmon (Salmo salar) mononuclear phagocytes |
title_sort |
cpg- and lps-activated mapk signaling in in vitro cultured salmon (salmo salar) mononuclear phagocytes |
publisher |
Elsevier |
publishDate |
2013 |
url |
https://hdl.handle.net/10037/5884 http://dx.doi.org710.1016/j.fsi.2013.07.014 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
Fish and Shellfish Immunology 35(2013) nr. 4 s. 1079-1085 FRIDAID 1072659 http://dx.doi.org710.1016/j.fsi.2013.07.014 1050-4648 https://hdl.handle.net/10037/5884 URN:NBN:no-uit_munin_5578 |
op_rights |
openAccess |
_version_ |
1766182725624004608 |