Retinal vascular calibres: Risk factors and methodological aspects of retinal vascular imaging. The Tromsø Eye Study – a part of the Tromsø Study.

• Main Author: Hanno, Therese von
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: UiT Norges arktiske universitet 2014
• Subjects: VDP::Medisinske Fag: 700::Helsefag: 800::Epidemiologi medisinsk og odontologisk statistikk: 803; VDP::Medical disciplines: 700::Health sciences: 800::Epidemiology medical and dental statistics: 803; VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Oftalmologi: 754; VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Ophthalmology: 754; The Tromsø Study; Tromsøundersøkelsen; DOKTOR-003; Broderstad; Hanno; Tromsø
• Online Access: https://hdl.handle.net/10037/5732

The papers of this thesis are not available in Munin: 1. von Hanno T, Bertelsen G, Sjølie AK, Mathiesen EB.: 'Retinal vascular calibres are significantly associated with cardiovascular risk factors: the Tromsø Eye Study', Acta Ophthalmologica (2013), Online ahead of print. Available at http://dx.doi.org/10.1111/aos.12102 2. von Hanno T, Bertelsen G, Broderstad AR, Wilsgaard T, Mathiesen EB.: 'Serum ferritin and hemoglobin are independently associated with wider retinal venular caliber. The Tromsø Study 2001-2008', Investigative Ophthalmology & Visual Science (2013), vol. 54(10):7053-7060. Available at http://dx.doi.org/10.1167/iovs.13-12204 3. von Hanno T, Sjølie AK, Mathiesen EB.: 'Retinal vascular calibre and response to light exposure and serial imaging', Acta Ophthalmologica (2013), Online before print. Available at http://dx.doi.org/10.1111/aos.12213 Cardiovascular disease is a major cause of death and morbidity in developed countries. The eye offers a unique window to the study of the microvasculature in vivo, which has been less investigated than the macrovasculature. We wanted to investigate the relationship between the traditional cardiovascular risk factors and retinal vascular calibres. Further, iron is a proposed cardiovascular risk factor and we wanted to investigate whether measures of iron stores and hemoglobin are related to retinal vascular calibre. The two first papers in this thesis are based on data from the Tromsø Study. We have measured the diameter of the retinal arterioles and venules on retinal images from 6353 participants of the sixth survey of the Tromsø Study (Tromsø 6, 2007-2008). We found that blood pressure and smoking were the factors with the most pronounced effect on the retinal vascular calibre. Blood pressure and age were associated with narrower retinal vessels and mainly affecting arteriolar calibre. Smoking, overweight (body mass index) and unfavourable lipid-profile were associated with wider retinal vessels, mainly affecting venular calibre. The effect of low HDL cholesterol and high BMI with venular widening was significantly stronger in men than in women. Further, 2993 participants with retinal vascular calibre measurements in Tromsø 6 also participated in the fifth survey (Tromsø 5, 2001-2002) when measurements of iron stores (serum ferritin and transferring saturation) and hemoglobin were performed. Men have higher levels of both iron stores and hemoglobin. We found that serum ferritin was associated with wider retinal venules in men. Hemoglobin was associated with wider retinal venules in both men and women. Cameras used for retinal photography commonly use light in the visual spectrum for the imaging technique and include several exposures with flash illumination to capture different fields of the retina. We examined 32 healthy volunteers and found that retinal venular calibre increased during an image sequence of 6 images and that the venular calibre was wider after light exposure compared to dark exposure before imaging.