Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes
Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eo...
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Online Access: | https://hdl.handle.net/10037/5618 https://doi.org/10.1371/journal.pone.0055893 |
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ftunivtroemsoe:oai:munin.uit.no:10037/5618 2023-05-15T15:30:32+02:00 Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes Kumari, Jaya Bøgwald, Jarl Dalmo, Roy Ambli 2013 https://hdl.handle.net/10037/5618 https://doi.org/10.1371/journal.pone.0055893 eng eng Public Library of Science (PLoS) PLoS ONE 8(2013) nr. 2 s. - PLoS ONE (2013), vol. 8(2): e55893 FRIDAID 1032405 http://dx.doi.org/10.1371/journal.pone.0055893 1932-6203 https://hdl.handle.net/10037/5618 URN:NBN:no-uit_munin_5309 openAccess VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 Journal article Tidsskriftartikkel Peer reviewed 2013 ftunivtroemsoe https://doi.org/10.1371/journal.pone.0055893 2021-06-25T17:53:42Z Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eomes cDNA (2477 bp). Promoter analysis of the salmon Eomes showed the presence of important putative transcription binding sites like SP1, FOXO, Oct-1, SMAD, STAT, IRF, and Ets-1. The basal core region responsible for the promoter activity was located between base 2199 and +59. Quantitative PCR analysis revealed that the Atlantic salmon Eomes was ubiquitously expressed in all the tissues studied but strongly expressed in the ovary, spleen, brain, and the head kidney. Moreover, the involvement of Eomes in Atlantic salmon immune response and its relation with the cytolytic activity was demonstrated by investigating the early time dependent expression profile of Eomes and CD8a followed by high interferon gamma (IFN-c) and granzyme A expression during challenge with live Aeromonas salmonicida and Infectious Pancreatic Necrosis (IPN) virus. Therefore, we further analyzed the regulated expression and function of this transcription factor in spleen lymphocytes. Overexpression of Eomes induced IFN-c, and granzyme A expression but not perforin expression, whereas small interfering RNA (siRNA) mediated suppression of Eomes expression led to significantly reduced IFN-c production. Thus, Eomes may be critical in cytolytic gene expression and function in fish similar to mammals. Furthermore, IFN-a, and mitogens induced Eomes expression. Taken together, this is the first study on the promoter activity and regulatory role of Eomes in fish. Article in Journal/Newspaper Atlantic salmon University of Tromsø: Munin Open Research Archive Aquaculture 289 3-4 327 333 |
institution |
Open Polar |
collection |
University of Tromsø: Munin Open Research Archive |
op_collection_id |
ftunivtroemsoe |
language |
English |
topic |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 |
spellingShingle |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 Kumari, Jaya Bøgwald, Jarl Dalmo, Roy Ambli Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
topic_facet |
VDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 |
description |
Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eomes cDNA (2477 bp). Promoter analysis of the salmon Eomes showed the presence of important putative transcription binding sites like SP1, FOXO, Oct-1, SMAD, STAT, IRF, and Ets-1. The basal core region responsible for the promoter activity was located between base 2199 and +59. Quantitative PCR analysis revealed that the Atlantic salmon Eomes was ubiquitously expressed in all the tissues studied but strongly expressed in the ovary, spleen, brain, and the head kidney. Moreover, the involvement of Eomes in Atlantic salmon immune response and its relation with the cytolytic activity was demonstrated by investigating the early time dependent expression profile of Eomes and CD8a followed by high interferon gamma (IFN-c) and granzyme A expression during challenge with live Aeromonas salmonicida and Infectious Pancreatic Necrosis (IPN) virus. Therefore, we further analyzed the regulated expression and function of this transcription factor in spleen lymphocytes. Overexpression of Eomes induced IFN-c, and granzyme A expression but not perforin expression, whereas small interfering RNA (siRNA) mediated suppression of Eomes expression led to significantly reduced IFN-c production. Thus, Eomes may be critical in cytolytic gene expression and function in fish similar to mammals. Furthermore, IFN-a, and mitogens induced Eomes expression. Taken together, this is the first study on the promoter activity and regulatory role of Eomes in fish. |
format |
Article in Journal/Newspaper |
author |
Kumari, Jaya Bøgwald, Jarl Dalmo, Roy Ambli |
author_facet |
Kumari, Jaya Bøgwald, Jarl Dalmo, Roy Ambli |
author_sort |
Kumari, Jaya |
title |
Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
title_short |
Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
title_full |
Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
title_fullStr |
Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
title_full_unstemmed |
Eomesodermin of Atlantic Salmon: An Important Regulator of Cytolytic Gene and Interferon Gamma Expression in Spleen Lymphocytes |
title_sort |
eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://hdl.handle.net/10037/5618 https://doi.org/10.1371/journal.pone.0055893 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
PLoS ONE 8(2013) nr. 2 s. - PLoS ONE (2013), vol. 8(2): e55893 FRIDAID 1032405 http://dx.doi.org/10.1371/journal.pone.0055893 1932-6203 https://hdl.handle.net/10037/5618 URN:NBN:no-uit_munin_5309 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0055893 |
container_title |
Aquaculture |
container_volume |
289 |
container_issue |
3-4 |
container_start_page |
327 |
op_container_end_page |
333 |
_version_ |
1766360977819828224 |